MICROFLUIDIC, ELECTROCHEMICAL DEVICES
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Abstract
Microfluidic, electrochemical devices are described. The microfluidic, electrochemical device comprises one or more electrode(s) on a substrate and a patterned porous, hydrophilic layer having a fluid-impermeable barrier which substantially permeates the thickness of the porous, hydrophilic layer and defines boundaries of one or more hydrophilic channels within the patterned porous, hydrophilic layer, wherein the hydrophilic channel(s) comprises a hydrophilic region which is in fluidic communication with the electrode(s). In some embodiments, the electrodes comprise a working electrode, a counter electrode, and a reference electrode. In some embodiments, the microfluidic, electrochemical device further comprises a fluid sink. The method of assembling the microfluidic, electrochemical device is described. The method of using the device for electrochemical analysis of one or more analytes is also described.
38 Citations
60 Claims
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1-30. -30. (canceled)
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31. A microfluidic, electrochemical analysis device comprising:
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a porous, hydrophilic layer comprising a fluid sample deposition region and a patterned, fluid impermeable boundary that substantially permeates the thickness of the hydrophilic layer and defines one or more hydrophilic channels therewithin in fluidic communication with said deposition region which permit fluidic flow within the one or more channels; and an electrode assembly comprising one or more electrodes in fluidic communication with said deposition region through a said channel. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60)
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57. The method of 51 wherein the step of measuring an electrical parameter is done by a method selected from the group consisting of amperometry, biamperometry, stripping voltammetry, differential pulse voltammetry, cyclic voltammetry, coulometry, chronoamperometry, and potentiometry.
Specification