LACTOBACILLUS AND STREPTOCOCCUS PROMOTERS AND USES THEREOF
First Claim
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1. A recombinant nucleic acid comprising a promoter, being a native promoter from a Lactobacillus species or a functional variant or functional fragment thereof, operably linked to one or more open reading frames heterologous to the promoter, wherein the promoter is selected from the group consisting of the native promoters of genes of Lactobacillus, preferably of Lactobacillus rhamnosus, for:
- 1) ribosomal protein S14 and ribosomal protein S17 and ribosomal protein L15 and ribosomal protein S3 (rpsJ),
2) nucleoid DNA-binding protein (dnabp),
3) ribosomal protein S21 (rpS21),
4) 50S ribosomal protein L19 (rplS),
5) 50S ribosomal protein L17 (map40),
6) 50S ribosomal protein L13 (rplM),
7) phosphoglycerate mutase 1 (pgml),
8) ribosomal protein S4 (rpS4),
9) glyceraldehyde-3-phosphate dehydrogenase (cggr), and functional variants and functional fragments of said native promoters or a recombinant nucleic acid comprising a promoter, being a native promoter from a Streptococcus species or a functional variant or functional fragment thereof, operably linked to one or more open reading frames heterologous to the promoter, wherein the promoter is selected from the group consisting of the native promoters of genes of Streptococcus, preferably of Streptococcus mutans, for;
10) 30S ribosomal protein S10,
11) 50S ribosomal protein L27,
12) 30S ribosomal protein S15,
13) 30S ribosomal protein S16,
14) 50S ribosomal protein L19,
15) 30S ribosomal protein S8,
16) 50S ribosomal protein L18 and 30S ribosomal protein S5,
17) 30S ribosomal protein S9,
18) 50S ribosomal protein L17,
19) 30S ribosomal protein S13,
20) 30S ribosomal protein S7,
21) 505 ribosomal protein L15,
22) 30S ribosomal protein S4,
23) 50S ribosomal protein L6,
24) 30S ribosomal protein S3 and 505 ribosomal protein L3,
25) phosphoglyceromutase, and functional variants and functional fragments of said native promoters.
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Abstract
The invention is in the field of molecular biology, and relates to recombinant engineering and protein expression. More in particular, the invention relates to nucleic acids for recombinant expression of proteins comprising sequences derived from Lactobacillus or Streptococcus and useful as promoters. The invention further relates to vectors comprising the said nucleic acids and host cells transformed therewith. The invention also covers the use of host cells comprising the said nucleic acids or vectors for expressing heterologous or homologous proteins; and also for delivery, especially therapeutic delivery, of the said proteins to subjects.
4 Citations
20 Claims
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1. A recombinant nucleic acid comprising a promoter, being a native promoter from a Lactobacillus species or a functional variant or functional fragment thereof, operably linked to one or more open reading frames heterologous to the promoter, wherein the promoter is selected from the group consisting of the native promoters of genes of Lactobacillus, preferably of Lactobacillus rhamnosus, for:
- 1) ribosomal protein S14 and ribosomal protein S17 and ribosomal protein L15 and ribosomal protein S3 (rpsJ),
2) nucleoid DNA-binding protein (dnabp),
3) ribosomal protein S21 (rpS21),
4) 50S ribosomal protein L19 (rplS),
5) 50S ribosomal protein L17 (map40),
6) 50S ribosomal protein L13 (rplM),
7) phosphoglycerate mutase 1 (pgml),
8) ribosomal protein S4 (rpS4),
9) glyceraldehyde-3-phosphate dehydrogenase (cggr), and functional variants and functional fragments of said native promoters or a recombinant nucleic acid comprising a promoter, being a native promoter from a Streptococcus species or a functional variant or functional fragment thereof, operably linked to one or more open reading frames heterologous to the promoter, wherein the promoter is selected from the group consisting of the native promoters of genes of Streptococcus, preferably of Streptococcus mutans, for;
10) 30S ribosomal protein S10,
11) 50S ribosomal protein L27,
12) 30S ribosomal protein S15,
13) 30S ribosomal protein S16,
14) 50S ribosomal protein L19,
15) 30S ribosomal protein S8,
16) 50S ribosomal protein L18 and 30S ribosomal protein S5,
17) 30S ribosomal protein S9,
18) 50S ribosomal protein L17,
19) 30S ribosomal protein S13,
20) 30S ribosomal protein S7,
21) 505 ribosomal protein L15,
22) 30S ribosomal protein S4,
23) 50S ribosomal protein L6,
24) 30S ribosomal protein S3 and 505 ribosomal protein L3,
25) phosphoglyceromutase, and functional variants and functional fragments of said native promoters. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19, 20)
- 1) ribosomal protein S14 and ribosomal protein S17 and ribosomal protein L15 and ribosomal protein S3 (rpsJ),
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2. (canceled)
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16. (canceled)
Specification
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Current AssigneeActoGeniX N.V.
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Original AssigneeActoGeniX N.V.
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InventorsSteidler, Lothar, Van Huynegem, Lokeren
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Application NumberUS13/498,232Publication NumberTime in Patent OfficeDaysField of SearchUS Class Current424/93.2CPC Class CodesC07K 14/315 from Streptococcus (G), e.g...C07K 14/335 from Lactobacillus (G)C12N 15/746 for lactic acid bacteria (S...C12N 9/0008 acting on the aldehyde or o...C12N 9/90 Isomerases (5.)C12P 21/02 having a known sequence of ...
