Assay Preparation Plates, Fluid Assay Preparation and Analysis Systems, and Methods for Preparing and Analyzing Assays

• US 20120184037A1
• Filed: 02/14/2012
• Published: 07/19/2012
• Est. Priority Date: 01/25/2008
• Status: Abandoned Application

• Current Assignee: Luminex Corporation (DiaSorin S.p.A.)
• Original Assignee: Luminex Corporation (DiaSorin S.p.A.)
• Inventors: Schilffarth, Adam, Deicher, William, Pempsell, Paul
• Application Number:
  US13/396,228
• Publication Number:
  US 20120184037A1
• Time in Patent Office: Days
• US Class Current: 436/50
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: H02N 15/00 Holding or levitation devices using magnetic attraction or repulsion, not otherwise provided for electric or magnetic devices for holding work on machine tools B23Q3/15 ; monorail vehicle propulsion or suspension B60L13/00; sliding or levitation devices for railway systems B61B13/08; material handling devices associated with conveyors incorporating devices with electrostatic or magnetic grippers B65G47/92; separating thin or filamentary articles from piles using magnetic force B65H3/16; delivering thin or filamentary articles from magnetic holders by air blast or suction B65H29/24; bearings using magnetic or electric supporting means F16C32/04; relieving bearing loads using magnetic means F16C39/06; magnets H01F7/00; dynamo-electric clutches or brakes H02K49/00 ; electric furnaces with simultaneous levitation and heating H05B6/32
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/115831 Condition or time responsive

Abstract

A fluid assay preparation and analysis system is provided which includes a pipette disposed above an assay plate receiving area, a magnet disposed below the assay plate receiving area in approximate alignment with the pipette, and an actuator configured to move the magnet proximate the assay plate receiving area. A method for preparing and analyzing an assay includes injecting a sample into a sample well of an assay preparation plate and inserting the assay preparation plate into a fluid assay analysis system. The method further includes mixing the sample with one or more reagents in an assay plate receiving area of the system and subsequently aspirating the prepared assay into an examination chamber of the system.

Claims

1. A method for preparing and analyzing an assay, comprising:

injecting a sample for analysis into a sample well of an assay preparation plate;

inserting the assay preparation plate into an assay plate receiving area of a fluid assay analysis system;

establishing a position of the assay preparation plate within the assay plate receiving area such that a particular well of the assay preparation plate is aligned with a pipette of the fluid assay analysis system;

aspirating a fluidic material disposed within the particular well via the pipette;

moving the assay preparation plate within the assay plate receiving area such that a different well of the assay preparation plate is aligned with the pipette;

dispensing the fluidic material into the different well, wherein the steps of establishing, aspirating, moving, and dispensing comprise mixing the sample with a reagent;

repeating the steps of establishing, aspirating, moving, and dispensing to mix the sample with one or more additional reagents until preparation of the assay is complete, wherein at least one series of the steps of establishing, aspirating, moving, and dispensing comprises mixing the sample with a plurality of magnetic particles;

immobilizing the plurality of magnetic particles in a well of the assay preparation plate subsequent to mixing the sample with the plurality of magnetic particles;

aspirating the assay from the assay preparation plate into an examination chamber of the fluid assay system via the pipette and a fluidic line coupled between the pipette and the examination chamber; and

analyzing the assay within the examination chamber.

2. The method of claim 1, wherein the steps of establishing, aspirating, moving, and dispensing comprise preparing the assay in the sample well.

3. The method of claim 1, wherein the steps of establishing, aspirating, moving, and dispensing comprise preparing the assay in a series of wells within the assay preparation plate.

4. The method of claim 1, wherein the step of immobilizing the plurality of magnetic particles comprises:

moving the assay preparation plate within the assay plate receiving area such that the pipette is aligned with a probe sensor of the assay preparation plate;

lowering the pipette down to the probe sensor; and

upon detecting the pipette with the probe sensor;

moving the assay preparation plate within the assay plate receiving area such that the pipette is aligned with the well of the assay preparation plate comprising the magnetic particles; and

actuating a magnet in proximity to the well comprising the magnetic particles.

5. The method of claim 1, further comprising respectively injecting one or more additional samples for analysis into other sample wells of the assay preparation plate prior to the step of inserting the assay preparation plate into an assay plate receiving area.

6. The method of claim 5, wherein the steps of establishing, aspirating, moving, and dispensing further comprise preparing respective assays for each of one or more additional samples in parallel with the preparation of the assay.

7. The method of claim 5, reiterating the steps of establishing, aspirating, moving, and dispensing to serially prepare respective assays for each of one or more additional samples.

8. The method of claim 1, wherein analyzing the assay within the examination chamber comprises illuminating the examination chamber and collecting light emitted or scattered by the assay.