Device and Method for Isolating Biological or Chemical Targets

• US 20120190037A1
• Filed: 05/17/2010
• Published: 07/26/2012
• Est. Priority Date: 05/19/2009
• Status: Active Grant

• Current Assignee: Commissariat a L'Energie Atomique (French Alternative Energies and Atomic Energy Commission)
• Original Assignee: Commissariat a L'Energie Atomique (French Alternative Energies and Atomic Energy Commission)
• Inventors: Durin, Guillaume, Delattre, Cyril, Jary, Dorothée, Chabaud, Sylvaine
• Granted Patent: US 8,703,434 B2
• Time in Patent Office: Days
• US Class Current: 435/6.15
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/56911 Bacteria

Abstract

The present invention relates to a device for collecting and concentrating biological or chemical targets with a view to detecting same, wherein the device comprises a reaction chamber (1) in which functionalized magnetic beads (2) are placed, an ultrasonic agitation system (3), a magnetic field (4) that can be activated (ON position) or inactivated (OFF position), and a fluid movement system (5) for feeding (51) and discharging (52) a portion or the entirety of the contents of the reaction chamber.

The present invention also relates to a method for capturing biological or chemical targets using said device.

Claims

1. An automatic device for processing a liquid sample having a volume V greater than or equal to 10 ml, comprising a reaction chamber, having a volume v of between 10 μ

A and 5 ml, in which functionalized magnetic beads are placed, said beads allowing the capture of biological or chemical targets in an amount such that the ratio of the capture surface area of the beads to the volume of the reaction chamber is between 0.2 and 200 m²/l, said reaction chamber being equipped;

with a noninvasive agitation system enabling homogeneous dispersion of said beads;

with a magnetic field that can be activated or inactivated;

with a fluid movement system for feeding and discharging a portion or the entirety of the contents of the reaction chamber.

2. The device as claimed in claim 1, wherein the reaction chamber has a volume of preferably between 50 and 500 and preferentially approximately 100 μ

l.

3. The device as claimed in claim 1, wherein the reaction chamber is coated with a hydrophobic surface chosen from Teflon, ETFE, PFA and PTFE.

4. The device as claimed in claim 1, wherein said noninvasive agitation system is an ultrasonic agitation system.

5. The device as claimed in claim 1, wherein said magnetic field is produced by a permanent magnet or an electromagnet.

6. The device as claimed in claim 1, wherein said functionalized magnetic beads have a diameter of between 0.5 and 10 μ

M.

7. The device as claimed in claim 1, wherein the device also comprises a liquid-sample collecting system consisting of a fluidic system for taking the sample, and of a storage reservoir upstream of the reaction chamber.

8. A method for capturing biological or chemical targets that may be present in a liquid sample having a volume V greater than or equal to 10 ml, with a device as claimed in claim 1, comprising the steps of:

a) taking a fraction of volume v of said liquid sample;

b) bringing said fraction into contact with functionalized magnetic beads in the reaction chamber of said device where the magnetic field is deactivated;

c) homogeneously dispersing the beads by means of the noninvasive agitation system for a period of at least 5 seconds;

d) capturing the beads by means of an activated magnetic field;

e) discharging said fraction;

f) repeating steps a) to e) a number of times n such that n is between 1 and V/v.

9. The capture method as claimed in claim 8, characterized in that n=V/v.

10. A method for detecting biological or chemical targets, comprising steps a) to f) of the capture method as claimed in claim 8, characterized in that it also comprises steps:

g) of eluting said targets and h) of detecting said targets eluted in step g).

11. The method for detecting biological targets as claimed in claim 10, wherein step h) consists in lysing said targets and in detecting the nucleic acids released during the lysis.