Processes And Systems For Enzymatically Isolating Lignin And Other Bioproducts From Herbaceous Plants

• US 20120190092A1
• Filed: 01/17/2012
• Published: 07/26/2012
• Est. Priority Date: 01/24/2011
• Status: Abandoned Application

• Current Assignee: Buckman Laboratories International, Inc (Bulab Holdings Incorporated)
• Original Assignee: Buckman Laboratories International, Inc (Bulab Holdings Incorporated)
• Inventors: Jaquess, Percy, Janse, Bernard
• Application Number:
  US13/351,285
• Publication Number:
  US 20120190092A1
• Time in Patent Office: Days
• US Class Current: 435/162
• CPC Class Codes: C12P 17/181 Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C12P 7/22 aromatic
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
• CPC Class Codes: C12Y 302/01008 Endo-1,4-beta-xylanase (3.2.1.8)
• CPC Class Codes: C12Y 302/0102 Alpha-glucosidase (3.2.1.20)
• CPC Class Codes: C12Y 302/01021 Beta-glucosidase (3.2.1.21)
• CPC Class Codes: C12Y 302/01032 Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
• CPC Class Codes: C12Y 302/01074 Glucan 1,4-beta-glucosidase (3.2.1.74)
• CPC Class Codes: C12Y 302/01091 Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
• CPC Class Codes: C13K 1/02 obtained by saccharification of cellulosic materials manufacture of fodder A23K10/32
• CPC Class Codes: C13K 13/007 Separation of sugars provided for in subclass C13K C13K3/00 takes precedence
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

Methods for enzymatically isolating lignin and other bioproducts, such as fermentable sugars, from herbaceous plant materials, are described. The methods can provide improvements, such as increased product purity and reduced process energy requirements and product modifications and contamination. Systems for practicing the methods also are provided.

Claims

1. A method for isolating components of lignocellulosic material comprising:

comminuting lignocellulosic material to form comminuted lignocellulosic material;

enzymatically digesting a mixture comprising the comminuted lignocellulosic material, at least one blend of enzymes, and aqueous solution in the absence of any prior non-enzymatic lignin separation from the lignocellulosic material, for enzymatic hydrolysis liberating at least one C6 and/or C5 sugar from an insoluble portion of the lignocellulosic material comprising lignin; and

separating at least a portion of the aqueous solution containing the at least one C6 and/or C5 sugar from the insoluble portion comprising lignin.

2. The method of claim 1, wherein the aqueous solution comprises an aqueous acidic organic buffer solution.

3. The method of claim 1, wherein the at least one C6 and/or C5 sugar in the aqueous solution comprises solubilized C6 and/or C5 sugar, or suspended C6 and/or C5 sugar particulate, or any combinations thereof.

4. The method of claim 1, wherein the enzymatically digesting of the comminuted lignocellulosic material in the aqueous solution with the enzyme blend comprises agitating the mixture for at least about 1 hour at a temperature of from about 30°

C. to about 60°

C., and the separating comprises discontinuing the agitating and decanting the at least a portion of the aqueous solution containing the at least one C6 and/or C5 sugar from the insoluble portion comprising settled solids.

5. The method of claim 1, wherein the lignocellulosic material is cotton seed hulls, grain hulls, sugarcane bagasse, corn stover, corn cobs, straw, switchgrass, leaves, stalks, plant shells, softwood pieces, hardwood pieces, sawdust, papermill pulp, waste paper, recycled paper, or any combinations thereof.

6. The method of claim 1, wherein the lignocellulosic material comprises cotton seed hulls.

7. The method of claim 1, wherein the at least one enzyme blend comprises a first enzyme blend comprising at least one enzyme from at least two of groups 1) to 5):

1) endoglucanase, carboxymethylcellulase;

2) exoglucanase, avicelase;

3) cellobiase, beta-glucosidase, alpha-glucosidase;

4) endo 1,4-beta-xylanase, endo-(1,4)-beta xylanohydrolase;

5) beta-1,3-xylanase, 1,3-beta-D-xylosidase, and exo-1,3-beta-xylosidase.

8. The method of claim 7, wherein first enzyme blend comprises:

at least one enzyme from groups

     1) and/or

     2),

at least one enzyme of group

     3), and

at least one enzyme of groups

     4) and/or

     5).

9. The method of claim 1, wherein the at least one enzyme blend further comprises a second enzyme blend comprising at least one enzyme from at least two of groups 1a) to 20a):

1a) endo-1,3(4)-beta-glucanase;

2a) laminarinase (endo-1,3-beta-glucanase);

3a) exo-1,2-1,6-alpha-mannosidase;

4a) beta-D-xylopyranosyl-(1,4)-beta-D-xylopyranose;

5a) alpha-N-arabinofuranosidase;

6a) feruloyl esterase;

7a) endo-1,5-alpha-arabinanase;

8a) pectinase;

9a) polygalacturonase;

10a) pectin esterase;

11a) aspartic protease;

12a) metallo protease;

13a) endo-(1,4)-mannanase;

14a) phytase;

15a) alpha-glucuronidase and beta-glucuronidase;

16a) hexenuronidase;

17a) alkaline phosphatase and acid phosphatase;

18a) alpha-galactosidase and beta-galactosidase;

19a) beta-mannosidase;

20a) alpha-fucosidase.

10. The method of claim 1, wherein yield of lignin is at least about 90%.

11. The method of claim 1, wherein yield of C6 and/or C5 sugars is at least about 90%.

12. A method for isolating components of lignocellulosic material comprising:

a) comminuting lignocellulosic material to a comminuted lignocellulosic material;

b) enzymatically digesting a first mixture comprising the comminuted lignocellulosic material, a first enzyme blend, and a first aqueous solution in the absence of any prior non-enzymatic lignin separation treatment of the lignocellulosic material, for a first enzymatic hydrolysis liberating at least one C6 and/or C5 sugar from a first insoluble portion of the lignocellulosic material comprising lignin;

c) separating at least a portion of the first aqueous solution containing the at least one C6 and/or C5 sugar from the first insoluble portion;

d) comminuting the first insoluble portion to a comminuted first insoluble portion;

e) enzymatically digesting a second mixture comprising the comminuted first insoluble portion, a second enzyme blend, and a second aqueous solution, for a second enzymatic hydrolysis liberating at least one C6 and/or C5 sugar from a second insoluble portion comprising lignin; and

f) separating the second insoluble portion comprising lignin from the second aqueous solution comprising the at least one C6 and/or C5 sugar.

13. The method of claim 12, wherein the comminuting of the lignocellulosic material comprises milling the lignocellulosic material to a size less than about 2 mm.

14. The method of claim 12, wherein the comminuting of the first insoluble portion comprises milling or grinding the first insoluble portion to a size less than about 50 microns.

15. The method of claim 12, further comprising, after the separating, washing the first insoluble portion at least once and drying the washed first insoluble portion before the comminuting of the first insoluble portion.

16. The method of claim 12, wherein the first aqueous solution comprises an aqueous acidic organic buffer solution.

17. The method of claim 12, wherein the at least one C6 and/or C5 sugar in the first aqueous solution comprises solubilized C6 and/or C5 sugar, or suspended C6 and/or C5 sugar particulate, or any combinations thereof.

18. The method of claim 12, wherein the enzymatically digesting of the comminuted lignocellulosic material in the first aqueous solution with the first enzyme blend comprises agitating the first mixture for at least about 1 hour at a temperature of from about 30°

C. to about 60°

C., and the separating comprises discontinuing the agitating and decanting the at least a portion of the first aqueous solution containing the at least one C6 and/or C5 sugar from the first insoluble portion comprising settled solids.

19. The method of claim 12, wherein the lignocellulosic material is cotton seed hulls, grain hulls, sugarcane bagasse, corn stover, corn cobs, straw, switchgrass, leaves, stalks, plant shells, softwood pieces, hardwood pieces, sawdust, papermill pulp, waste paper, recycled paper, or any combinations thereof.

20. The method of claim 12, wherein the lignocellulosic material comprises cotton seed hulls.

21. The method of claim 12, wherein the first enzyme blend comprises at least one enzyme from at least two of groups 1) to 5):

1) endoglucanase, carboxymethylcellulase;

2) exoglucanase, avicelase;

3) cellobiase, beta-glucosidase, alpha-glucosidase;

4) endo 1,4-beta-xylanase, endo-(1,4)-beta xylanohydrolase;

5) beta-1,3-xylanase, 1,3-beta-D-xylosidase, and exo-1,3-beta-xylosidase.

22. The method of claim 12, wherein the second enzyme blend comprises at least one enzyme from at least two of groups 1a) to 20a):

1a) endo-1,3(4)-beta-glucanase;

2a) laminarinase (endo-1,3-beta-glucanase);

3a) exo-1,2-1,6-alpha-mannosidase;

4a) beta-D-xylopyranosyl-(1,4)-beta-D-xylopyranose;

5a) alpha-N-arabinofuranosidase;

6a) feruloyl esterase;

7a) endo-1,5-alpha-arabinanase;

8a) pectinase;

9a) polygalacturonase;

10a) pectin esterase;

11a) aspartic protease;

12a) metallo protease;

13a) endo-(1,4)-mannanase;

14a) phytase;

15a) alpha-glucuronidase and beta-glucuronidase;

16a) hexenuronidase;

17a) alkaline phosphatase and acid phosphatase;

18a) alpha-galactosidase and beta-galactosidase;

19a) beta-mannosidase;

20a) alpha-fucosidase.

23. The method of claim 12, wherein yield of lignin is at least about 90%.

24. The method of claim 12, wherein yield of the at least one C6 and/or C5 sugar is at least about 50% based on the original amount present in the lignocellulosic material.

25. The method of claim 12, further comprising biofermentation processing of the at least one C6 and/or C5 sugar.

26. A system for isolating components of lignocellulosic material comprising:

at least one comminutor for comminuting lignocellulosic material;

at least one digestor comprising a vessel equipped with an internal agitator, wherein the vessel is operable for holding a mixture of the comminuted lignocellulosic material, at least one enzyme blend, and aqueous solution in the absence of any prior non-enzymatic lignin separation treatment of the lignocellulosic material, and operable for enzymatically digesting the mixture with agitation for enzymatic hydrolysis liberating at least one C6 and/or C5 sugar from an insoluble portion of the lignocellulosic material comprising lignin; and

a separator for separating at least a portion of the aqueous solution containing the at least one sugar from the insoluble portion comprising lignin.

27. The system of claim 26, further comprising:

a dryer for drying the insoluble portion comprising lignin;

a comminutor for comminuting the first insoluble material;

a digestor comprising a vessel equipped with an internal agitator, wherein the vessel is operable for holding a mixture of the comminuted first insoluble material, at least one enzyme, and aqueous solution, and contacting the mixture with agitation for enzymatic hydrolysis liberating at least one C6 and/or C5 sugar from an insoluble portion of the lignocellulosic material comprising lignin; and

a separator for separating at least a portion of the aqueous solution containing the at least one sugar from the insoluble portion comprising lignin.

28. The system of claim 26, further comprising a biofermentation processing system adapted to receive the liberated C6 and/or C5 sugar from the digestor and ferment the C6 and/or C5 sugar into ethanol.