METHOD FOR COMBINED IMAGING AND TREATING ORGANS AND TISSUES
First Claim
1. A method for in vivo diagnosis, visualization or therapy of residual diseased tissue in a subject in need thereof comprising:
- (a) illuminating an in vivo exposed body cavity, integument, or organ of a subject containing diseased tissue with light having at least one excitation wavelength in the range from about 401 nm to about 510 nm;
(b) directly viewing the illuminated cavity, integument, or organ with or without the aid of an endoscope fluorescence emitted from the body cavity or organ in response to the light from a fluorescent targeting construct comprising a fluorophore-tagged tumor-specific antibody or a fluorophore-tagged tumor avid moiety and a therapeutic isotope molecule, wherein the fluorophore-tagged antibody or fluorophore-tagged tumor avid moiety is responsive to the excitation wavelength administered to the subject and which has specifically bound to and/or been taken up by the diseased tissue in the exposed body cavity or organ; and
(c) determining by direct visualization without the aid of a capture device the location and/or surface area of the diseased tissue in the subject from the fluorescence provided by the targeting construct.
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Abstract
The present invention provides methods and compositions for detecting and treating malignant tissue, organs or cells in a mammal. The method comprises parenterally injecting a mammalian subject, at a locus or by a route providing access to the tissue or organ, with a composition comprising a monoclonal antibody (chimeric, humanized, fully human), partial antibody, Fab Fragment, antibody fragment that is tagged with a fluorophore with or without the addition of a therapeutic chemotherapy molecule, which specifically binds to the targeted organ, tissue or cell. Resection of the primary malignant tissue within the mammalian species (using the fluorescence of the fluorescing targeting construct) provides the advantage of identifying all bulk tumor as fluorescent at the time of the original tumor resection.
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Citations
36 Claims
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1. A method for in vivo diagnosis, visualization or therapy of residual diseased tissue in a subject in need thereof comprising:
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(a) illuminating an in vivo exposed body cavity, integument, or organ of a subject containing diseased tissue with light having at least one excitation wavelength in the range from about 401 nm to about 510 nm; (b) directly viewing the illuminated cavity, integument, or organ with or without the aid of an endoscope fluorescence emitted from the body cavity or organ in response to the light from a fluorescent targeting construct comprising a fluorophore-tagged tumor-specific antibody or a fluorophore-tagged tumor avid moiety and a therapeutic isotope molecule, wherein the fluorophore-tagged antibody or fluorophore-tagged tumor avid moiety is responsive to the excitation wavelength administered to the subject and which has specifically bound to and/or been taken up by the diseased tissue in the exposed body cavity or organ; and (c) determining by direct visualization without the aid of a capture device the location and/or surface area of the diseased tissue in the subject from the fluorescence provided by the targeting construct. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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32. A method for utilizing a diagnostic procedure during surgery in a subject in need thereof comprising:
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(a) illuminating an in vivo exposed body cavity or organ of the subject exposed by surgery and that contains diseased tissue with light having at least one excitation wavelength in the range from about 401 nm to about 510 nm; (b) directly viewing with or without the aid of an endoscope or robotic device, the fluorescence emanating from a fluorescent targeting construct comprising an fluorophore-tagged antibody or a fluorophore-tagged tumor avid moiety and a therapeutic isotope molecule, wherein the fluorophore-tagged antibody or fluorophore-tagged tumor avid moiety is responsive to the excitation wavelength which is administered to the subject and which has specifically bound to and/or been taken up by the diseased tissue in the body cavity or organ, and wherein the targeting construct fluoresces in response to the at least one excitation wavelength; (c) determining the location and/or surface area of the diseased tissue in the subject from the directly viewed fluorescence from the targeting construct; and (d) removing all or at least a portion of the diseased tissue. - View Dependent Claims (33, 34)
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35. A method for in vivo diagnosis of tumor cells in a subject in need thereof comprising:
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(a) contacting samples of tumor cells obtained from the subject in vitro with a plurality of detectably labeled compounds, each of which binds to one or more cell-surface tumor antigens or is selectively taken up by a distinct tumor type; (b) determining which of the compounds is bound to cell-surface tumor antigens or taken up by the sample tumor cells; (c) administering to the subject a diagnostically effective amount of at least one biologically compatible fluorescing targeting construct containing a compound determined in (b) that is tagged with a therapeutic isotope molecule to bind to and/or be taken up by exposed tumor tissue or organ that matches the sample tumor cells, which targeting construct fluoresces in response to light having at least one excitation wavelength in the range from about 401 nm to about 510 nm; and (d) diagnosing the location and/or surface area of the exposed tumor tissue in the in vivo body cavity or organ by directly viewing with or without the aid of an endoscopic device for viewing the fluorescence emanating from the targeting construct bound to or taken up in the exposed tumor tissue or organ in response to illumination with the excitation light source. - View Dependent Claims (36)
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Specification