DETECTION SYSTEM FOR DROPLET-BASED ASSAYS
First Claim
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1. A method of detection for droplets, comprising:
- illuminating an examination region of a channel with first pulses of light interleaved with second pulses of light as droplets pass through the examination region, the first pulses being spectrally distinct from the second pulses; and
collecting data representing light detected during illumination of the examination region with the first pulses and the second pulses.
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Abstract
System, including methods and apparatus, for light detection and signal processing for droplet-based assays.
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Citations
20 Claims
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1. A method of detection for droplets, comprising:
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illuminating an examination region of a channel with first pulses of light interleaved with second pulses of light as droplets pass through the examination region, the first pulses being spectrally distinct from the second pulses; and collecting data representing light detected during illumination of the examination region with the first pulses and the second pulses. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A system for detection in droplet-based assays, comprising:
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a channel; an illumination assembly configured to produce a beam of light that illuminates an examination region of the channel as droplets pass through such region; a detector configured to detect light received from the examination region; and a controller that collects data representing light detected by the detector, wherein the beam of light is elongated in cross section where the beam intersects the channel. - View Dependent Claims (14, 15, 16, 17, 18, 19)
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20. A method of detection for droplet-based assays, comprising:
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generating at least two separate signals each representing light detected with a different detection configuration during a series of time intervals from a stream of fluid carrying droplets; combining the at least two separate signals to form a combined signal; and processing the combined signal to identify time intervals that correspond to droplets.
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Specification