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SYSTEM, METHOD, AND APPARATUS FOR MEASURING AFFINITY CONSTANTS

  • US 20120202296A1
  • Filed: 02/02/2012
  • Published: 08/09/2012
  • Est. Priority Date: 02/02/2011
  • Status: Abandoned Application
First Claim
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1. A non-linear spectroscopic method of measuring an affinity constant without labeling, the method comprising:

  • providing a plurality of free, charged biomolecules in an aqueous solution;

    providing a plurality of bound biomolecules, the bound biomolecules being bound to respective surfaces of a plurality of polymer beads;

    complexing the free charged biomolecules with the bound biomolecules;

    probing the complexed biomolecules using second harmonic generation (“

    SHG”

    );

    detecting a change in a net electric charge for solid/aqueous interfaces associated with the complexed biomolecules based on an SHG signal received from the solution; and

    determining an affinity constant of the complexed biomolecules based on said detecting.

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