METHODS FOR MANIPULATING LIQUID SUBSTANCES IN MULTI-CHAMBERED RECEPTACLES
First Claim
1. A method of establishing fluid communication between chambers of a receptacle, the method comprising the steps of:
- (a) in a first chamber of the receptacle, forming a volume comprising a liquid substance and an immiscible liquid; and
(b) applying a substance-moving force to the volume of the first chamber sufficient to alter an openable connection between the first chamber and a second chamber of the receptacle from a closed state to an open state, wherein the volume in the first chamber would be insufficient for the substance-moving force to alter the openable connection from the closed state to the open state absent the immiscible liquid.
5 Assignments
0 Petitions
Accused Products
Abstract
A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.
4 Citations
47 Claims
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1. A method of establishing fluid communication between chambers of a receptacle, the method comprising the steps of:
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(a) in a first chamber of the receptacle, forming a volume comprising a liquid substance and an immiscible liquid; and (b) applying a substance-moving force to the volume of the first chamber sufficient to alter an openable connection between the first chamber and a second chamber of the receptacle from a closed state to an open state, wherein the volume in the first chamber would be insufficient for the substance-moving force to alter the openable connection from the closed state to the open state absent the immiscible liquid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A method of altering the temperature of a liquid substance contained in a chamber of a receptacle, the method comprising the steps of:
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(a) forming a volume in a first chamber of a receptacle so that a flexible portion of a first chamber wall expands to contact a stationary thermal element positioned adjacent to the first chamber, wherein the volume comprises a liquid substance and a corresponding immiscible liquid, and wherein the immiscible liquid causes the flexible portion of the first chamber wall to expand to contact the thermal element to a greater extent than if the immiscible liquid was absent from the first chamber; and (b) altering the temperature of the liquid substance in the first chamber by transmitting thermal energy between the thermal element and the volume. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
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Specification