NOVEL EUROPEAN PRRSV STRAIN
First Claim
Patent Images
1. A Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) of a European type, which is of the strain deposited with European Collection of Cell Cultures (ECACC) under the Accession Numbers ECACC 11012501 or Accession Numbers ECACC 11012502.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention is related to improved modified live PRRS vaccines containing new PRRSV European strains of PRRSV and methods of use and manufacture of such vaccines.
23 Citations
27 Claims
- 1. A Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) of a European type, which is of the strain deposited with European Collection of Cell Cultures (ECACC) under the Accession Numbers ECACC 11012501 or Accession Numbers ECACC 11012502.
- 14. A method of immunizing swine against porcine reproductive and respiratory syndrome (PRRS), said method comprising the step of administering to swine a vaccine composition including a live porcine reproductive and respiratory syndrome virus mixed with a pharmacologically compatible carrier agent, said virus comprising PRRS 94881 virus passaged at least 36 times in cell culture to modify the virus such that when the modified virus is administered to a swine or other mammal prone to PRRS, it fails to cause clinical signs of PRRS disease but is capable of inducing an immune response that immunizes the mammal against pathogenic forms of PRRS.
-
17. A PRRS virus having a nucleotide sequence that is at least 95% homologous with the sequence set forth in either SEQ ID NO:
- 1 or SEQ ID NO;
10.
- 1 or SEQ ID NO;
-
18. A PRRS virus that comprises at least one ORF that encodes a protein that is at least 98% identical to any of the sequences set forth in SEQ ID NO:
- 2 to 9 or SEQ ID NO;
11 to SEQ ID NO;
18.
- 2 to 9 or SEQ ID NO;
-
19. A PRRS virus that has a nucleotide sequence of SEQ ID NO:
- 1 or SEQ ID NO;
10 or a fragment of either SEQ ID NO;
1 or SEQ ID NO;
2 wherein said fragment encodes an ORF selected from the group consisting of SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
8, SEQ ID NO;
9, SEQ ID NO;
11, SEQ ID NO;
12, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
15, SEQ ID NO;
16, SEQ ID NO;
17, and SEQ ID NO;
18.
- 1 or SEQ ID NO;
-
20. A subunit vaccine for vaccination of a porcine animal wherein said vaccine comprises one or more nucleotides selected from the group consisting of a nucleotide that encodes an ORF selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
8, SEQ ID NO;
9, SEQ ID NO;
11, SEQ ID NO;
12, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
15, SEQ ID NO;
16, SEQ ID NO;
17, and SEQ ID NO;
18.
- 2, SEQ ID NO;
-
21. A subunit vaccine for vaccination of a porcine animal wherein said vaccine comprises one or more nucleotides selected from the group consisting of SEQ ID NO:
- 19;
SEQ ID NO;
20;
SEQ ID NO;
21;
SEQ ID NO;
22;
SEQ ID NO;
23;
SEQ ID NO;
24;
SEQ ID NO;
25;
SEQ ID NO;
26;
SEQ ID NO;
27;
SEQ ID NO;
28;
SEQ ID NO;
29;
SEQ ID NO;
30;
SEQ ID NO;
31;
SEQ ID NO;
32;
SEQ ID NO;
33; and
SEQ ID NO;
34.
- 19;
-
22. A composition comprising one or more proteins selected from the group consisting of a protein having the sequence of SEQ ID NO:
- 2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
8, SEQ ID NO;
9, SEQ ID NO;
11, SEQ ID NO;
12, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
15, SEQ ID NO;
16, SEQ ID NO;
17, and SEQ ID NO;
18.
- 2, SEQ ID NO;
-
23. An isolated nucleic acid comprising a sequence selected from the group consisting of SEQ ID NO:
- 19;
SEQ ID NO;
20;
SEQ ID NO;
21;
SEQ ID NO;
22;
SEQ ID NO;
23;
SEQ ID NO;
24;
SEQ ID NO;
25;
SEQ ID NO;
26;
SEQ ID NO;
27;
SEQ ID NO;
28;
SEQ ID NO;
29;
SEQ ID NO;
30;
SEQ ID NO;
31;
SEQ ID NO;
32;
SEQ ID NO;
33; and
SEQ ID NO;
34.
- 19;
-
24. A recombinant expression vector comprising a nucleic acid sequence that encodes one or more PRRSV ORFs selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
8, SEQ ID NO;
9, SEQ ID NO;
11, SEQ ID NO;
12, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
15, SEQ ID NO;
16, SEQ ID NO;
17, and SEQ ID NO;
18 operably linked to a promoter. - View Dependent Claims (25, 26, 27)
- 2, SEQ ID NO;
Specification