METHODS OF CULTURING CELLS IN A MEDIUM COMPRISING TRANSFORMING GROWTH FACTOR BETA 1 AND BASIC FIBROBLAST GROWTH FACTOR
First Claim
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1. A method of propagating pluripotent stem cells in an undifferentiated, pluripotent and proliferative state under culturing conditions devoid of feeder cells, the method comprising culturing the pluripotent stem cells in a tissue culture medium which comprises transforming growth factor β
-
1 (TGFβ
1) and basic fibroblast growth factor (bFGF), to thereby maintain the pluripotent stem cells in an undifferentiated, pluripotent and proliferative state.
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Abstract
The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
4 Citations
26 Claims
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1. A method of propagating pluripotent stem cells in an undifferentiated, pluripotent and proliferative state under culturing conditions devoid of feeder cells, the method comprising culturing the pluripotent stem cells in a tissue culture medium which comprises transforming growth factor β
-
1 (TGFβ
1) and basic fibroblast growth factor (bFGF), to thereby maintain the pluripotent stem cells in an undifferentiated, pluripotent and proliferative state. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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1 (TGFβ
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15. A cell culture comprising pluripotent stem cells in a culture medium, wherein the cell culture is substantially free of animal- and/or feeder cells—
- contaminants and whereas said stem cells are maintained in an undifferentiated, pluripotent and proliferative state when cultured in said culture medium.
- View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24)
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25. A method of propagating cells in a culture, comprising culturing the cells in a culture medium which comprises transforming growth factor β
-
1 (TGFβ
1) and basic fibroblast growth factor (bFGF), wherein said bFGF is provided at a concentration of at least 2 ng/ml, and wherein said TGFβ
1 is provided at a concentration range of 0.06-0.24 ng/ml, there propagating the cells in the culture.
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1 (TGFβ
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26. A cell culture comprising cells and a culture medium, wherein said culture medium comprises transforming growth factor β
-
1 (TGFβ
1) and basic fibroblast growth factor (bFGF), wherein said bFGF is provided at a concentration of at least 2 ng/ml, and wherein said TGFβ
1 is provided at a concentration range of 0.06-0.24 ng/ml.
-
1 (TGFβ
Specification