COMPOSITION, DEVICE AND METHOD FOR COLORIMETRIC DETECTION OF AN ANALYTE USING IMPRINTED POLYMERS AND PHOTOCHROMIC SWITCH MOLECULES

• US 20120214252A1
• Filed: 10/26/2010
• Published: 08/23/2012
• Est. Priority Date: 10/30/2009
• Status: Active Grant

• Current Assignee: Medtech Detect, LLC
• Original Assignee: Medtech Detect, LLC
• Inventors: Knop, Richard H.
• Granted Patent: US 8,945,938 B2
• Time in Patent Office: Days
• US Class Current: 436/164
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

The invention provides various compositions, devices, and methods useful for detecting an analyte by colorimetric readout. The compositions and devices include polymers constructed to have pores specifically imprinted to provide for capture of an analyte in the pores; nanoparticles layered on the polymers; and photochromic optical switch molecules coated on the nanoparticles. Capture of the analyte in the pores of the polymer results in a change in the spatial relation of the nanoparticles layered on the polymer, which in turn causes the photochromic optical switch molecules coated thereon to undergo a change in energy state corresponding to a visible change in the optical spectra of the photochromic optical switch molecules.

Claims

1. A composition for use in detecting an analyte in a sample by colorimetric optical readout, comprising:

(a) a polymer having pores specifically imprinted to provide for capture of an analyte in said pores;

(b) nanoparticles layered on said polymer; and

(c) photochromic optical switch molecules coated on said nanoparticles,

wherein capture of the analyte in the pores of the polymer results in a change in the spatial relation of the nanoparticles layered on the polymer, said change in spatial relation of the nanoparticles causing the photochromic optical switch molecules coated thereon to undergo a change in energy state which corresponds to a visible change in optical spectra of said photochromic optical switch molecules, thereby providing detection of the analyte by colorimetric optical readout.

4. An article for detecting an analyte in a sample by colorimetric optical readout, said article comprising a composition according to claim 1 capable of detecting by colorimetric optical readout an analyte contained in a sample contacted with said article.

5. A composition according to claim 1 for use in detecting an analyte by colorimetric optical readout.

6. Use of a composition according to claim 1 for the manufacture of a device or article capable of detecting an analyte by colorimetric optical readout.

2. A device for detection of an analyte in a sample by colorimetric optical readout, comprising:

(a) a chamber for containing a sample that includes an analyte; and

(b) a composition positioned in the chamber, the composition including;

(i) a polymer having pores specifically imprinted to provide for capture of the analyte in said pores;

(ii) nanoparticles layered on said polymer; and

(iii) photochromic optical switch molecules coated on said nanoparticles,

wherein capture of the analyte in the pores of the polymer results in a change in the spatial relation of the nanoparticles layered on the polymer, said change in spatial relation of the nanoparticles causing the photochromic optical switch molecules coated thereon to undergo a change in energy state which corresponds to a visible change in optical spectra of said photochromic optical switch molecules, thereby providing detection of the analyte by colorimetric optical readout.

3. A method of detecting an analyte in a sample by colorimetric optical readout, comprising:

(a) contacting a sample that includes an analyte with a composition comprising;

(i) a polymer having pores specifically imprinted to provide for capture of the analyte in said pores;

(ii) nanoparticles layered on said polymer; and

(iii) photochromic optical switch molecules coated on said nanoparticles;

(b) capturing the analyte in the pores of the polymer; and

(c) detecting the analyte,

wherein said capture of the analyte results in a change in the spatial relation of the nanoparticles layered on the polymer, said change in spatial relation of the nanoparticles causing the photochromic optical switch molecules coated thereon to undergo a change in energy state which corresponds to a visible change in optical spectra of said photochromic optical switch molecules, thereby providing detection of the analyte by colorimetric optical readout.

7. A device for detection of an analyte in a sample by colorimetric optical readout, comprising:

(a) a chamber for containing a sample that includes an analyte; and

(b) a means for detecting said analyte by colorimetric optical readout.