Reducing Non-Covalently Bound Polysaccharide On Supports
First Claim
1. A method of reducing an amount of non-covalently bound polysaccharide on a support, the method comprising contacting a support comprising both covalently bound polysaccharide and non-covalently bound polysaccharide with an aqueous solution comprising an amount of a chaotropic agent effective to remove non-covalently bound polysaccharide from the support.
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Accused Products
Abstract
Methods and reagents are disclosed for reducing an amount of non-covalently bound polysaccharide on a support. The method comprises contacting a support comprising both covalently bound polysaccharide and non-covalently bound polysaccharide with an aqueous solution comprising an amount of a chaotropic agent effective to remove non-covalently bound polysaccharide from the support.
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Citations
20 Claims
- 1. A method of reducing an amount of non-covalently bound polysaccharide on a support, the method comprising contacting a support comprising both covalently bound polysaccharide and non-covalently bound polysaccharide with an aqueous solution comprising an amount of a chaotropic agent effective to remove non-covalently bound polysaccharide from the support.
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10. A method of preparing a conjugate of a support and an assay molecule, said method comprising:
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(a) treating a support under conditions for covalently binding a polysaccharide to the support, (b) contacting the support with an aqueous solution comprising an amount of a chaotropic agent effective to remove non-covalently bound polysaccharide from the support, (c) separating the support from the aqueous solution, and (d) contacting the support with an assay molecule under conditions for binding of the assay molecule to the covalently bound polysaccharide. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
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18. A method of reducing an amount of non-covalently bound dextran or dextran derivative on a latex particle, the method comprising:
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(a) contacting a latex particle comprising both covalently bound dextran derivative and non-covalently bound dextran derivative with an aqueous solution comprising an amount of one or more chaotropic agents effective to remove non-covalently bound dextran derivative from the support, and (b) separating the latex particle from the aqueous solution by contacting the aqueous solution with a membrane wherein substantially all of the non-covalently bound dextran derivative passes through the membrane. - View Dependent Claims (19, 20)
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Specification