COMPOSITIONS AND METHODS OF GENERATING REPROGRAMMED ADIPOCYTE CELLS AND METHODS OF USE THEREFORE
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Abstract
The invention provides therapeutic compositions comprising reprogrammed adipocyte cells for use as disease models, therapeutic compositions comprising reprogrammed adipocyte cells for the treatment of conditions characterized by a reduction in cell number or tissue mass, and methods of generating such cells.
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Citations
71 Claims
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1-46. -46. (canceled)
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47. A method for generating a reprogrammed adipocyte, the method comprising
exogenously expressing in a pluripotent stem cell one or more adipogenic transcription factor polypeptides; - and
contacting the cell with one or more of insulin, rosiglitazone, dexamethasone and isobutylmethylxanthine, thereby generating a reprogrammed adipocyte. - View Dependent Claims (48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 67, 68, 71)
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63. A reprogrammed adipocyte that exogenously expresses an adipogenic transcription factor polypeptide selected from the group consisting of PPARγ
- , CREB1, SREBF1, KLF5, KLF15, KROX20, C/EBPβ
, C/EBPδ
C/EBPα
, and CDEC, wherein the expression confers adipocyte-marker expression, adipocyte morphology and/or adipocyte function.
- , CREB1, SREBF1, KLF5, KLF15, KROX20, C/EBPβ
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64. A reprogrammed adipocyte that exogenously expresses a PPARγ
- 2 or C/EBPα
polypeptide, wherein the expression confers adipocyte-marker expression, adipocyte morphology and/or adipocyte function.
- 2 or C/EBPα
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65. A reprogrammed adipocyte that exogenously expresses a polypeptide selected from the group consisting of C/EBPα
- , C/EBPβ and
C/EBPδ
, wherein the expression confers adipocyte-marker expression, adipocyte morphology and/or adipocyte function. - View Dependent Claims (66)
- , C/EBPβ and
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69. A collection of at least two expression vectors, wherein each vector comprises a distinct nucleic acid sequence encoding a polypeptide selected from the group consisting of PPARγ
- 2, C/EBPα
, C/EBPβ
, C/EBPδ
, SREBP1c, CREB1 and KROX20. - View Dependent Claims (70)
- 2, C/EBPα
Specification