Microcarriers for Stem Cell Culture
First Claim
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1. A method of generating bone tissue in vivo comprising implanting cells into a human or animal at a location where bone growth is required, wherein the cells have been obtained by the in vitro suspension culture of mesenchymal stem cells attached to microcarriers.
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Abstract
The present application discloses a method of generating bone tissue in vivo comprising implanting cells into a human or animal at a location where bone growth is required, wherein the cells have been obtained by the in vitro suspension culture of mesenchymal stem cells attached to microcarriers.
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Citations
14 Claims
- 1. A method of generating bone tissue in vivo comprising implanting cells into a human or animal at a location where bone growth is required, wherein the cells have been obtained by the in vitro suspension culture of mesenchymal stem cells attached to microcarriers.
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6. A method of generating bone tissue in vivo, the method comprising:
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(i) attaching mesenchymal stem cells to a plurality of microcarriers to form microcarrier-stem cell complexes, (ii) culturing the microcarrier-mesenchymal stem cell complexes in suspension culture, (iii) separating cultured cells from the microcarriers, (iv) implanting the separated cells into a human or animal at a location where bone growth is required. - View Dependent Claims (7, 8, 9)
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10. A method of culturing mesenchymal stem cells in suspension culture in vitro and causing their differentiation to an osteogenic lineage, the method comprising:
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(i) attaching mesenchymal stem cells to a plurality of microcarriers to form microcarrier-stem cell complexes, (ii) culturing the microcarrier-mesenchymal stem cell complexes in suspension culture, (iii) inducing differentiation of the mesenchymal stem cells obtained from (ii), wherein the method comprises placing the microcarrier-mesenchymal stem cell complexes under conditions which induce the osteogenic differentiation of mesenchymal stem cells.
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11. Cells which have been obtained, produced or identified by a method of culturing mesenchymal stem cells in suspension culture in vitro, the method comprising:
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(i) attaching mesenchymal stem cells to a plurality of microcarriers to form microcarrier-stem cell complexes; and (ii) culturing the microcarrier-mesenchymal stem cell complexes in suspension culture. - View Dependent Claims (12, 13, 14)
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Specification