SYSTEMS AND METHODS FOR HIGH-THROUGHPUT DETECTION OF AN ANALYTE IN A SAMPLE

• US 20120231960A1
• Filed: 03/09/2012
• Published: 09/13/2012
• Est. Priority Date: 03/09/2011
• Status: Active Grant

• Current Assignee: MagArray, Inc.
• Original Assignee: MagArray, Inc.
• Inventors: Osterfeld, Sebastian J., Wang, Shan X.
• Granted Patent: US 9,151,763 B2
• Time in Patent Office: Days
• US Class Current: 506/7
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00655 the compounds being bound to magnets embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 9/523 for multisample carriers, e.g. used for microtitration plates
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 60/12 for screening libraries
• CPC Class Codes: G01N 27/3276 being a hybridisation with immobilised receptors using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68
• CPC Class Codes: G01N 27/745 for detecting magnetic beads used in biochemical assays concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098
• CPC Class Codes: G01N 33/585 with a particulate label, e.g. coloured latex
• CPC Class Codes: G01N 33/587 Nanoparticles
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/026 having blocks or racks of reaction cells or cuvettes
• CPC Class Codes: G01R 33/0094 Sensor arrays
• CPC Class Codes: G01R 33/1269 of molecules labeled with magnetic beads magnetic particles for bio assay G01N33/54326
• CPC Class Codes: Y10S 977/773 Nanoparticle, i.e. structure having three dimensions of 100 nm or less
• CPC Class Codes: Y10S 977/92 Detection of biochemical

Abstract

Provided are high-throughput detection systems. The systems include a magnetic sensor device, a magnetic field source and a reservoir plate that includes a plurality of fluid reservoirs. The magnetic sensor device includes a support with two or more elongated regions each having a magnetic sensor array disposed at a distal end. Also provided are methods in which the subject high-throughput detection systems find use.

Claims

1. A high-throughput detection system comprising:

(a) a magnetic sensor device comprising a support with two or more elongated regions each having a magnetic sensor array disposed at a distal end;

(b) a magnetic field source; and

(c) a reservoir plate comprising a plurality of fluid reservoirs,

wherein the system is configured to position the magnetic sensor arrays in sequential sets of the fluid reservoirs.

2. The system of claim 1, wherein each magnetic sensor array comprises one or more magnetic sensors having an analyte-specific probe bound to a surface of the magnetic sensor.

3. The system of claim 1, further comprising a first reservoir plate actuator configured to move the reservoir plate into an operative relationship with the magnetic sensor device such that the distal ends of the elongated regions of the magnetic sensor device are each positioned in separate fluid reservoirs.

4. The system of claim 3, further comprising a second reservoir plate actuator configured to move the reservoir plate along at least one axis coplanar with the reservoir plate.

5. The system of claim 1, further comprising a magnetic sensor device actuator configured to move the magnetic sensor device into an operative relationship with the magnetic field source.

6. The system of claim 1, further comprising a processor configured to obtain a real-time analyte-specific signal from the magnetic sensor array.

7. The system of claim 6, wherein the processor is configured to obtain the real-time analyte-specific signal from the magnetic sensor array as a magnetically-labeled analyte in a sample binds to an analyte-specific probe or as a magnetic label binds to an analyte/analyte-specific probe complex.

8. A high-throughput detection system comprising:

(a) a magnetic field source; and

(b) a first reservoir plate actuator configured to position a reservoir plate having a plurality of fluid reservoirs into an operative relationship with a magnetic sensor device comprising two or more magnetic sensor arrays,

wherein the system is configured to position the two or more magnetic sensor arrays in sequential sets of the fluid reservoirs.

9. The system of claim 8, further comprising a second reservoir plate actuator configured to move the reservoir plate along at least one axis coplanar with the reservoir plate.

10. The system of claim 8, further comprising a magnetic sensor device actuator configured to move the magnetic sensor device into an operative relationship with the magnetic field source.

11. The system of claim 8, further comprising a processor configured to obtain a real-time analyte-specific signal from the magnetic sensor arrays.

12. A magnetic sensor device comprising:

a support with two or more elongated regions each having a magnetic sensor array disposed at a distal end.

13. The device of claim 12, wherein each magnetic sensor array comprises one or more magnetic sensors having an analyte-specific probe bound to a surface of the magnetic sensor.

14. The device of claim 12, wherein each distal end of the elongated regions is sized to fit within separate fluid reservoirs.

15. The device of claim 13, wherein at least one of the magnetic sensor arrays comprises two or more distinct magnetic sensors each configured to specifically detect the same analyte.

16. The device of claim 13, wherein at least one of the magnetic sensor arrays comprises two or more distinct magnetic sensors each configured to specifically detect a different analyte.

17. The device of claim 13, wherein at least two of the magnetic sensor arrays are configured to specifically detect a different set of analytes.

18. The device of claim 12, wherein the magnetic sensor array comprises one or more spin valve sensors.

19. The device of claim 12, wherein the magnetic sensor array comprises one or more magnetic tunnel junction sensors.

20-24. -24. (canceled)

25. A method for determining whether an analyte is present in a sample, the method comprising:

(a) contacting a magnetic sensor device with a set of samples contained in a set of fluid reservoirs to generate a signal, the magnetic sensor device comprising a support with two or more elongated regions each having a magnetic sensor array disposed at a distal end; and

(b) determining whether the analyte is present in each sample based on the signal.

26-35. -35. (canceled)