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CONTROL OF PESTS IN PLANTS

  • US 20120240288A1
  • Filed: 09/15/2010
  • Published: 09/20/2012
  • Est. Priority Date: 09/29/2009
  • Status: Active Grant
First Claim
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1. A method of screening pest genes to identify pest genes which can lead to mortality of the pest when expression of the pest gene is silenced in the pest, the method comprising:

  • (a) inserting a nucleic acid comprising a sequence of a first desired gene into a virus-induced gene silencing (VIGS) vector of a virus that can infect a desired host to produce a modified VIGS vector, wherein the first desired gene is a pest gene to be screened;

    (b) inoculating the host with the modified VIGS vector to produce infected host;

    (c) growing the infected host under conditions in which the modified VIGS vector is replicated to produce RNA which accumulates in tissue of the host;

    (d) feeding the host tissue with RNA to pests; and

    (e) determining whether the RNA is toxic to the pest,wherein pest toxicity identifies the pest gene as a pest gene that leads to mortality of the pest when the pest gene expression is silenced in the pest.

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