MULTIPLEX ANALYSIS OF CELLS, PARTICLES, AND OTHER ANALYTES
First Claim
1. A device comprising a housing having a fluid inlet that is in fluid communication with a plurality of channels, wherein each of said plurality of channels is in fluid communication with a reservoir containing reagents for analyzing cells, particles, or analytes bound to said particles, one or more substantially planar, porous membranes through which said cells or particles do not pass, and one or more outlets, wherein liquid flowing away from said inlet is divided between said plurality of channels and flows through said one or more membranes towards said outlet, and wherein said reservoir is disposed upstream of said one or more membranes.
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Accused Products
Abstract
In general, the invention features multiplexed devices, systems, methods, and kits for analysis of cells, particles, and other analytes on a porous membrane. Preferred devices detect, identify and quantify low levels of microorganisms in complex biological samples, such as blood. An exemplary device includes a housing having a fluid inlet that is in fluid communication with a plurality of channels, e.g., having substantially the same fluidic resistance. Each of the plurality of channels is in fluid communication with a reservoir containing reagents for analyzing cells, particles, or other analytes bound to particles, one or more substantially planar, porous membranes through which the cells or particles do not pass, and one or more outlets, wherein liquid flowing away from the inlet is divided between the plurality of channels and flows through the one or more membranes towards the outlet, and wherein the reservoir is disposed upstream of the one or more membranes.
20 Citations
49 Claims
- 1. A device comprising a housing having a fluid inlet that is in fluid communication with a plurality of channels, wherein each of said plurality of channels is in fluid communication with a reservoir containing reagents for analyzing cells, particles, or analytes bound to said particles, one or more substantially planar, porous membranes through which said cells or particles do not pass, and one or more outlets, wherein liquid flowing away from said inlet is divided between said plurality of channels and flows through said one or more membranes towards said outlet, and wherein said reservoir is disposed upstream of said one or more membranes.
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30. A method of analyzing a sample, said method comprising the steps of:
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(i) introducing said sample into a device comprising a housing having a fluid inlet that is in fluid communication with a plurality of channels, wherein each of said plurality of channels is in fluid communication with a reservoir containing reagents for analyzing cells, particles, or analytes bound to said particles, one or more substantially planar, porous membranes through which said cells or particles do not pass, and one or more outlets, wherein liquid flowing away from said inlet is divided between said plurality of channels and flows through said one or more membranes towards said outlet, and wherein said reservoir is disposed upstream of said one or more membranes, wherein said sample comprises said cells, particles or analytes that bind to said particles; (ii) allowing said reagents for analyzing to contact said cells, particles, or analytes; (iii) capturing said cells or particles on said membrane; and (iv) analyzing said cells or particles on said membrane. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
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49. A device that can detect, identify, and quantify a low-level of microorganisms in complex biological samples.
Specification