Biosensor Using Magnetic Microparticles

• US 20120251392A1
• Filed: 10/18/2010
• Published: 10/04/2012
• Est. Priority Date: 10/19/2009
• Status: Abandoned Application

• Current Assignee: Tokyo Institute of Technology (National University Corporation Tokyo Institute of Technology), Sumitomo Electric Industries Limited (Sumitomo Corporation)
• Original Assignee: Tokyo Institute of Technology (National University Corporation Tokyo Institute of Technology)
• Inventors: Sandhu, Adarsh
• Application Number:
  US13/502,707
• Publication Number:
  US 20120251392A1
• Time in Patent Office: Days
• US Class Current: 422/69
• CPC Class Codes: G01N 2021/1727 Magnetomodulation
• CPC Class Codes: G01N 21/1717 with a modulation of one or more physical properties of the sample during the optical investigation, e.g. electro-reflectance
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

Provided is a biosensor using magnetic microparticles whereby a biomaterial can be detected at a high sensitivity. The biosensor using magnetic microparticles is provided with a light source (10), a magnetic field generator (20), a light receiver (30) and a detector (40). The light source (10) irradiates a dispersion (1) containing the magnetic microparticles, to which the biomaterial to be detected can bind, with light of a definite wavelength. The magnetic field generator (20) applies to the dispersion a magnetic field capable of changing at least in two directions. The light receiver (30) receives transmitting light from the dispersion (1). The detector (40) detects the presence or absence of the target biomaterial based on the magnitude of a change in the quantity of the transmitting light, which changes in response to a change in the direction of the magnetic field applied by the magnetic field generator (20), received by the light receiver (30).

Claims

1. A biosensor that uses magnetic microparticles, the biosensor comprising:

a light source that emits light of a predetermined wavelength to a dispersion liquid that contains magnetic microparticles to which a target biomaterial can bind;

a magnetic field generation unit that is able to apply a magnetic field, a direction of which changes at least in two directions, to the dispersion liquid;

a light receiving unit that receives a transmitted light from the dispersion liquid; and

a detection unit that detects whether or not the target biomaterial exists based on an amount of change in a quantity of the transmitted light received by the light receiving unit, which is caused by a direction change of the magnetic field applied by the magnetic field generation unit.

2. The biosensor according to claim 1, in which the magnetic field generation unit is able to apply a rotating magnetic field.

3. The biosensor according to claim 1, in which a direction of the magnetic field applied by the magnetic field generation unit and a direction of the light emitted from the light source are directions within the same plane.

4. The biosensor according to claim 1, in which the detection unit further detects whether or not the target biomaterial exists based on a quantity of the transmitted light after the magnetic field generation unit stops applying the magnetic field.

5. The biosensor according to claim 1, in which the detection unit further detects whether or not the target biomaterial exists by using a calibration curve that is created in advance with the use of a known sample.

6. A biosensor that uses magnetic microparticles, the biosensor comprising:

a magnetic field generation unit that is able to apply a magnetic field, a direction of which changes at least in two directions, to a dispersion liquid that contains magnetic microparticles to which a target biomaterial can bind;

a light source that emits light of a predetermined wavelength to the dispersion liquid;

a light receiving unit that receives a light reflected from the dispersion liquid; and

a detection unit that detects whether or not the target biomaterial exists based on the amount of change in the quantity of the reflected light received by the light receiving unit, which is caused by a direction change of the magnetic field applied by the magnetic field generation unit.

7. The biosensor according to claim 6, which further comprising an optical waveguide that leads the light from the light source to the dispersion liquid.

8. The biosensor according to claim 7, in which the light receiving unit is disposed on a dispersion liquid-side tip of the optical waveguide.