Automated On-Instrument pH Adjustment

• US 20120258546A1
• Filed: 04/06/2012
• Published: 10/11/2012
• Est. Priority Date: 04/08/2011
• Status: Abandoned Application

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: MARRAN, David, BEAUCHEMIN, Mark
• Application Number:
  US13/441,565
• Publication Number:
  US 20120258546A1
• Time in Patent Office: Days
• US Class Current: 436/163
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

A method of preparing a sequencing device includes determining a sensitivity of a pH of a solution to a first reagent, determining an amount of the first reagent to add to the solution to approach a target pH, adding the amount of the first reagent to the solution, and diluting a nucleotide solution with the solution.

Claims

1. A method of preparing a sequencing device, the method comprising:

determining a sensitivity of a pH of a solution to a first reagent;

determining an amount of the first reagent to add to the solution to approach a target pH;

adding the amount of the first reagent to the solution; and

diluting a nucleotide solution with the solution.

2. The method of claim 1, wherein determining the sensitivity of the pH of the solution to the first reagent includes determining an initial pH of the solution.

3. The method of claim 2, wherein determining the initial pH of the solution includes determining the initial pH with an array of ion sensitive components disposed on a common substrate.

4. The method of claim 3, wherein the array forms a portion of a sequencing device.

5. The method of claim 2, wherein determining the sensitivity further comprises adding a first amount of a first reagent to the solution following determining the initial pH of the solution.

6. The method of claim 5, wherein determining the sensitivity further comprises determining a second pH of the solution following adding the first amount of the first reagent.

7. The method of claim 1, wherein determining the amount of the first reagent includes determining a scaled sensitivity.

9. The method of claim 1, further comprising determining a pH of the nucleotide solution.

11. The method of claim 1, further comprising adding an amount of a second reagent to the solution, the second reagent having a pH different from the first reagent.

12. The method of claim 1, further comprising determining a pH of the solution following adding the amount of the first reagent to the solution.

15. The method of claim 1, wherein the first solution is a wash solution of the sequencing device.

8. (canceled)

10. (canceled)

13. (canceled)

14. (canceled)

16. A method of preparing a sequencing device, the method comprising:

determining an initial pH of a solution;

adding a first amount of a first reagent to the solution;

determining a pH of the solution;

determining a second amount of the first reagent to add to the solution;

adding the second amount of the first reagent to the solution;

determining the pH of the solution; and

diluting a nucleotide solution with the solution.

17. The method of claim 16, wherein determining the initial pH of the solution includes determining the initial pH with an array of ion sensitive components disposed on a common substrate.

18. The method of claim 17, wherein the array forms a portion of a sequencing device.

19. The method of claim 16, wherein determining the second amount of the first reagent includes determining a scaled sensitivity.

21. The method of claim 16, further comprising determining a pH of the nucleotide solution.

23. The method of claim 16, further comprising adding an amount of a second reagent to the solution, the second reagent having a pH different from the first reagent.

20. (canceled)

22. (canceled)

24. A method of protocol monitoring, the method comprising:

from each of a plurality of sequencing devices, receiving an indication of an amount of a reagent added to a solution to adjust the pH of the solution to within a range of a target pH;

determining an error state based at least in part on the received indications; and

notifying an administrator of the determined error state.

25. The method of claim 24, wherein determining the error state includes determining that an amount of adjustment is in excess.

26. The method of claim 24, wherein determining the error state includes determining a site level error.

27-29. -29. (canceled)