Automated On-Instrument pH Adjustment
First Claim
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1. A method of preparing a sequencing device, the method comprising:
- determining a sensitivity of a pH of a solution to a first reagent;
determining an amount of the first reagent to add to the solution to approach a target pH;
adding the amount of the first reagent to the solution; and
diluting a nucleotide solution with the solution.
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Abstract
A method of preparing a sequencing device includes determining a sensitivity of a pH of a solution to a first reagent, determining an amount of the first reagent to add to the solution to approach a target pH, adding the amount of the first reagent to the solution, and diluting a nucleotide solution with the solution.
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Citations
27 Claims
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1. A method of preparing a sequencing device, the method comprising:
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determining a sensitivity of a pH of a solution to a first reagent; determining an amount of the first reagent to add to the solution to approach a target pH; adding the amount of the first reagent to the solution; and diluting a nucleotide solution with the solution. - View Dependent Claims (2, 3, 4, 5, 6, 7, 9, 11, 12, 15)
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8. (canceled)
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10. (canceled)
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13. (canceled)
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14. (canceled)
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16. A method of preparing a sequencing device, the method comprising:
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determining an initial pH of a solution; adding a first amount of a first reagent to the solution; determining a pH of the solution; determining a second amount of the first reagent to add to the solution; adding the second amount of the first reagent to the solution; determining the pH of the solution; and diluting a nucleotide solution with the solution. - View Dependent Claims (17, 18, 19, 21, 23)
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20. (canceled)
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22. (canceled)
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24. A method of protocol monitoring, the method comprising:
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from each of a plurality of sequencing devices, receiving an indication of an amount of a reagent added to a solution to adjust the pH of the solution to within a range of a target pH; determining an error state based at least in part on the received indications; and notifying an administrator of the determined error state. - View Dependent Claims (25, 26)
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27-29. -29. (canceled)
Specification