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ULTRAFAST SEQUENCING OF BIOLOGICAL POLYMERS USING A LABELED NANOPORE

  • US 20120261261A1
  • Filed: 03/21/2012
  • Published: 10/18/2012
  • Est. Priority Date: 09/30/2009
  • Status: Active Grant
First Claim
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1. A method for sequencing a polymer comprising:

  • exciting a donor label attached to a nanopore;

    translocating a polymer through the nanopore, wherein the polymer comprises a monomer labeled with an acceptor label;

    transferring energy from the excited donor label to the acceptor label of the monomer after the labeled monomer passes through and exits the nanopore;

    detecting the energy emitted by the acceptor label as a result of the energy transfer, wherein the energy emitted by the acceptor label is associated with a single monomer; and

    deducing the polymer sequence based on the detection of the emitted energy from the acceptor label and identification of the monomer.

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