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METHODS AND APPARATUS FOR POINT-OF-CARE NUCLEIC ACID AMPLIFICATION AND DETECTION

  • US 20120264116A1
  • Filed: 04/14/2012
  • Published: 10/18/2012
  • Est. Priority Date: 04/14/2011
  • Status: Active Grant
First Claim
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1. Apparatus for point-of-care amplification and detection of a target nucleic acid sequence, the apparatus comprising:

  • a sample collector; and

    a nucleic acid amplification instrument,wherein the nucleic acid amplification instrument comprises a sensor, a heating element, a display, and at least one reaction chamber having nucleic acid amplification reagents,wherein the sample collector is configured to collect and deliver to the at least one reaction chamber a sample matrix that may contain the target nucleic acid sequence,wherein the heating element is configured to heat the nucleic acid amplification reagents after delivery of the sample matrix to the at least one reaction chamber,wherein the nucleic acid amplification reagents are configured to amplify the target nucleic acid sequence within the sample matrix upon heating of the reagents with the heating element,wherein the sensor is configured to detect amplification of the target nucleic acid sequence, andwherein the display is configured to output detection results obtained with the sensor.

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