DETECTION OF BACTERIA IN BIOLOGICAL FLUIDS

• US 20120270248A1
• Filed: 04/21/2011
• Published: 10/25/2012
• Est. Priority Date: 04/21/2011
• Status: Abandoned Application

• Current Assignee: Pall Corporation (Danaher Corporation)
• Original Assignee: Pall Corporation (Danaher Corporation)
• Inventors: Kwan, Cyndi Leslie Chen
• Application Number:
  US13/091,446
• Publication Number:
  US 20120270248A1
• Time in Patent Office: Days
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose

Abstract

A method for detecting bacteria in biological fluids is provided, the method comprising placing a biological fluid possibly containing bacteria in a container, the container including a detergent for reducing the respiration of blood cells, and a bacterial growth promoter, and measuring and/or detecting the level of glucose in the biological fluid in the container, over a period of time. A system for carrying out the method is also provided.

Claims

1. A method for detecting bacteria in a biological fluid, the method comprising:

(a) placing a biological fluid, the biological fluid possibly containing bacteria, in a container, wherein the container includes (i) a detergent for reducing the respiration of blood cells, and (ii) a bacterial growth promoter; and

,

(b) measuring and/or detecting the level of glucose in the biological fluid in the container over a period of time, wherein a decrease in the level of glucose over the period of time indicates the presence of bacteria.

2. The method of claim 1, wherein the biological fluid comprises a platelet-containing fluid.

3. The method of claim 1, wherein the biological fluid comprises a stem cell-containing fluid.

4. The method of claim 1, wherein the biological fluid comprises a cell culture.

5. The method of claim 1, comprising measuring and/or detecting a first level of glucose in the container, and, within about 4 to about 15 hours of measuring and/or detecting the first level, measuring and/or detecting a second level of glucose in the container.

6. The method of claim 1, comprising obtaining a biological fluid from a subject, and, within about 24 hours or less of obtaining the biological fluid from the subject, passing a portion of the biological fluid into the container where the portion of biological fluid is placed in contact with the detergent and the bacterial growth promoter.

7. The method of claim 1, wherein the biological fluid comprises a leukocyte-depleted fluid.

8. A system for detecting bacteria in biological fluid, comprising:

(a) a biological fluid sampling device comprising a container suitable for containing a biological fluid, the container comprising a glucose access port, wherein the container contains (i) an effective amount of a detergent for reducing the respiration of blood cells, and (ii) an effective amount of a bacterial growth promoter; and

,

(b) a glucose measuring device and/or glucose reading device, wherein the device is suitable for, over a period of time, measuring or detecting a first level of glucose in the biological fluid, and a second level of glucose in the biological fluid, wherein a decrease in the level of glucose over the period of time indicates the presence of bacteria.