Observation Cell Arrangement
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Accused Products
Abstract
An observation cell arrangement for flow perfusion of a sample to be examined, the arrangement comprising a flow cell (21) having a cavity therein to receive the sample, the flow cell (21) arranged to receive a flow of fluid through the cavity that is directed over the sample from a cavity inlet (22) to a cavity outlet (23), the cavity inlet (22) associated with a fluid supply line, and a first flow supply path (24) connected to the fluid supply line via a valve (39), the first flow supply path (24) adapted to receive pressure from a pressure source comprising a pressure reservoir (29) to drive fluid flow through the cavity at a desired flow rate
7 Citations
63 Claims
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1-37. -37. (canceled)
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38. An observation cell arrangement for flow perfusion of a sample to be observed, the arrangement comprising:
a flow cell having a cavity therein to receive the sample, the cavity having a cavity inlet and a cavity outlet, the flow cell arranged to receive a flow of fluid through the cavity from the inlet to the outlet that is directed over the sample, the cavity inlet associated with a fluid delivery line, and a first flow supply path connected to the fluid delivery line via a valve, the arrangement including a pressure source to pressurise the first flow supply path, the pressure source comprising a reservoir adapted to store pressurised fluid. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
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51. A method of performing observations with a flow perfusion apparatus comprising the steps of;
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a) adding a systemic fluid to a first fluid vessel; b) charging a reservoir with pressurised fluid; c) adding a sample to be observed to a flow cell cavity; d) initiating a flow of fluid through the flow cell cavity using the pressure reservoir; e) examining the sample. - View Dependent Claims (52, 53, 54, 55, 56, 57, 58)
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59. A method of identifying a drug comprising the steps of:
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a) placing cultured cells in a flow cell; b) providing a systemic fluid flow at a first, substantially constant, flow rate over the cultured cells; c) introducing a dosing fluid flow of a first candidate drug while maintaining the substantially constant first flow rate over the cells; d) observing the effect of the drug on the cultured cells; e) identifying the drug as being effective if the effect on the cultured cells fulfils predetermined criteria. - View Dependent Claims (60, 61, 62, 63)
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Specification