Hybrid Separation and Detection Device for Chemical Detection and Analysis

• US 20120270330A1
• Filed: 08/25/2010
• Published: 10/25/2012
• Est. Priority Date: 09/14/2009
• Status: Abandoned Application

• Current Assignee: Arizona Board of Regents (University of Arizona)
• Original Assignee: Arizona Board of Regents (University of Arizona)
• Inventors: Tao, Nongjian, Forzani, Erica, Tsow, Francis, Iglesias, Rodrigo A
• Application Number:
  US13/395,625
• Publication Number:
  US 20120270330A1
• Time in Patent Office: Days
• US Class Current: 436/140
• CPC Class Codes: G01N 2030/8854 involving hydrocarbons
• CPC Class Codes: G01N 2291/0255 (Bio)chemical reactions, e.g. on biosensors
• CPC Class Codes: G01N 2291/0256 Adsorption, desorption, surface mass change, e.g. on biosensors
• CPC Class Codes: G01N 2291/0427 Flexural waves, plate waves, e.g. Lamb waves, tuning fork, cantilever
• CPC Class Codes: G01N 29/022 Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices microdevices per se B81B
• CPC Class Codes: G01N 29/036 by measuring frequency or resonance of acoustic waves
• CPC Class Codes: G01N 29/222 Constructional or flow details for analysing fluids optoacoustic fluid cells G01N29/2425
• CPC Class Codes: G01N 30/88 Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
• CPC Class Codes: Y10T 436/212 Aromatic

Abstract

The present invention provides a device that makes it possible to perform real-time detection and analysis of BTEX components in real samples using an inexpensive and miniaturized hybrid specific binding-separation device. The device may be used in occupational health and safety applications as well as for toxicological population studies to determine the presence of organic volatile components in an air sample.

Claims

1. A device for the detection of chemical moieties in a gas sample, said device comprising:

a. a flow control unit;

b. a separation unit;

c. a detection unit comprising at least one sensor comprising a coating of a sensing material that specifically detects the presence of said chemical moieties in said gas sample;

wherein said flow control unit is operably linked to and controls the flow of a mobile gas phase through the separation unit;

said separation unit is operably linked to said detection unit and separates the components in said gas sample and provides said separated components to said detection unit; and

a sensor to provide a signal to a detection circuit that allows a qualitative and/or quantitative detection of said chemical moieties separated by said separation unit.

2. The device of claim 1, wherein said sensor is a resonating tuning fork that allows a qualitative and/or quantitative detection of said chemical moieties separated by said separation unit.

3. The device of claim 1, wherein said flow control unit comprises a sample trap loop, a miniaturized valve for sample delivery to the device and a gas pump for introducing said mobile phase to said separation unit.

4. The device of claim 1, wherein said separation unit is a capillary gas chromatography column.

5. The device of claim 1, wherein said sensing material, is a highly cross-linked polymer material imprinted with aromatic hydrocarbons.

6. The device of claim 5, wherein said highly cross-linked polymer material is based on cross linked divinylbenzene.

7. The device of claim 5, wherein said aromatic hydrocarbon is selected from the group consisting of benzene, toluene, ethylbenzene, biphenyl, naphthalene, pyrene, and a monoaromatic or polyaromatic hydrocarbon.

8. The device of claim 1, wherein said tuning fork has a resonance frequency of about 32 kHz.

9. The device of claim 1, wherein said tuning fork is connected to a high performance digital counter wherein said counter allows measurement of a resonance frequency resolution less than 2 mHz.

10. The device of claim 1, wherein said high frequency resolution detection circuit comprises a chip to allow wireless capability.

11. The device of claim 1, wherein said detection unit comprises a plurality of resonating tuning forks, wherein different tuning forks comprising different coatings to selectively detect chemical moieties in said gas sample.

12. The device of claim 1, wherein said at least one resonating quartz crystal tuning fork comprising a coating of at least one sensing material that allows the detection of one or more of benzene, toluene, ethylbenzene, xylenes (BTEX) or aromatic, alkyl or halogenated hydrocarbons in said gas sample.

13. The device of claim 12, wherein said device further comprises a second resonating quartz crystal tuning fork comprising a coating of a sensing material that detects non-BTEX materials in said gas sample.

14. The device of claim 11, wherein said device comprises a coating selected from a polyaromatic hydrocarbon, polycarbosilane derivative, polysiloxane derivative, fluoroalcohol polycarbosilanes, polycarbosilane polysiloxanes, crown-ether derivatives, and graphite derivatives, and combinations thereof.

15. The device of claim 4, wherein said gas chromatography column is a gas chromatography column comprised of carbowax and cyanopropylphenyl silicone capillary columns connected in series.

16. The device of claim 1, wherein said device detects the presence of chemical moieties in air, and said chemical moieties are aromatic volatile organic compounds associated with traffic pollution.

17. The device of claim 4, wherein said device does not contain a column heating device for the chromatography columns.

18. The device of claim 4, wherein said device further comprises a heating element to heat the chromatography columns.

19. The device of claim 1, wherein said device further comprises a sampling unit comprising a column packed with a material for the preconcentration of analytes prior to loading of said analytes to said separation unit.

20. The device of claim 19, wherein said column is packed with a material selected from the group consisting of a highly cross-linked polymer material imprinted with aromatic hydrocarbons, molecular sieves, carbopack X, carbopack B, carboxen and/or amorphous carbon materials or combinations thereof.

21. The device of claim 1, wherein said device further comprises a zero filter for providing carrier gas to the separation device.

22. The device of claim 21, wherein said zero filter is comprised of activated carbon, a molecular sieve, alumina, silica, activated carbon, graphite, polymers or a strong oxidizing material.

23. The device of claim 1, wherein said device is a hand-held combined miniaturized gas-chromatograph and a detection apparatus comprising an array of quartz crystal tuning forks coated with molecularly imprinted polymers that specifically detect benzene, toluene, ethylbenzene and xylenes.

24. A method of detecting the presence of benzene, toluene, ethylbenzene and xylenes in a gas sample comprising performing a miniaturized gas chromatographic separation of said sample and detecting the presence of BTEX therein using a device of any of claims 1-24.