MICROFLUIDIC SYSTEM AND METHOD FOR AUTOMATED PROCESSING OF PARTICLES FROM BIOLOGICAL FLUID

• US 20120270331A1
• Filed: 04/20/2012
• Published: 10/25/2012
• Est. Priority Date: 04/20/2011
• Status: Abandoned Application

• Current Assignee: Achal Singh Achrol, Palaniappan Sethu, Richard S. Gaster
• Original Assignee: Achal Singh Achrol, Palaniappan Sethu, Richard S. Gaster
• Inventors: Achrol, Achal Singh, Sethu, Palaniappan, Gaster, Richard S.
• Application Number:
  US13/452,760
• Publication Number:
  US 20120270331A1
• Time in Patent Office: Days
• US Class Current: 436/177
• CPC Class Codes: B01L 2200/0652 Sorting or classification of particles or molecules
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: G01N 2001/4083 sedimentation
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A microfluidic system for automatically depleting particles not of interest from a biological sample, comprising: a sampling module configured to receive the sample; and one or more microfluidic protein and nucleic acid depletion modules fluidically coupled to the sampling module and comprising binding agents configured to selectively bind to abundant plasma proteins or nucleic acids. A method for automatically depleting particles not of interest from a sample, comprising: receiving the sample; subjecting the sample to a force that separates at least a portion of the particles not of interest from the sample, thereby isolating at least a portion of the target component; passing the isolated target copmonent into a chamber; circulating the isolated target component in the chamber; and selectively capturing proteins or nucleic acids with binding agents within the chamber.

Claims