METHODS FOR IDENTIFYING FACTORS FOR DIFFERENTIATING DEFINITIVE ENDODERM
First Claim
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1. An in vitro cell culture comprising a medium, human definitive endoderm cells, and human pluripotent stem cells, wherein said definitive endoderm cells are multipotent cells that can differentiate into cells of the gut tube or organs derived therefrom, and wherein said human pluripotent stem cells comprise less than 5% of the cells in said cell culture.
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Abstract
Disclosed herein are methods of identifying one or more differentiation factors that are useful for differentiating cells in a cell population comprising definitive endoderm cells into cells which are capable of forming tissues and/or organs that are derived from the gut tube.
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20 Claims
- 1. An in vitro cell culture comprising a medium, human definitive endoderm cells, and human pluripotent stem cells, wherein said definitive endoderm cells are multipotent cells that can differentiate into cells of the gut tube or organs derived therefrom, and wherein said human pluripotent stem cells comprise less than 5% of the cells in said cell culture.
- 4. The cell culture of claim 4, wherein said embryonic stem cell is derived from a gondal ridge of an embryo.
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14. An in vitro cell culture comprising a medium, human definitive endoderm cells, and human pluripotent stem cells, wherein at least 15% of said human cells are definitive endoderm cells, said definitive endoderm cells being multipotent cells that can differentiate into cells of the gut tube or organs derived therefrom.
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