MAGNETIC BEADS FOR REDUCING LEUKOCYTE INTERFERENCE IN IMMUNOASSAYS
First Claim
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1. A method of reducing interference from leukocytes in an analyte immunoassay, comprising:
- contacting a biological sample containing leukocytes with magnetic sacrificial beads opsonized to said leukocytes under conditions sufficient to bind at least a portion of said leukocytes in the biological sample to the magnetic sacrificial beads; and
magnetically retaining the magnetic sacrificial beads substantially out of contact from an immunosensor.
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Abstract
Methods and devices for reducing interference from leukocytes in an analyte immunoassay are provided. In one embodiment, a method is provided comprising the steps of amending a biological sample with magnetic sacrificial beads opsonized to leukocytes, binding leukocytes in the sample to the magnetic sacrificial beads, and magnetically retaining the beads out of contact from an immunosensor.
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Citations
54 Claims
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1. A method of reducing interference from leukocytes in an analyte immunoassay, comprising:
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contacting a biological sample containing leukocytes with magnetic sacrificial beads opsonized to said leukocytes under conditions sufficient to bind at least a portion of said leukocytes in the biological sample to the magnetic sacrificial beads; and magnetically retaining the magnetic sacrificial beads substantially out of contact from an immunosensor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method of performing an analyte sandwich immunoassay in a blood sample, comprising:
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introducing a blood sample containing leukocytes into a holding chamber in a test cartridge, wherein a portion of the holding chamber is coated with magnetic sacrificial beads opsonized to leukocytes; contacting the blood sample with the magnetic sacrificial beads under conditions sufficient to bind at least a portion of the leukocytes in the biological sample to the magnetic sacrificial beads; moving the blood sample into a conduit, wherein a magnetic field localized to at least a portion of the conduit attracts and retains the magnetic sacrificial beads on at least a portion of the wall of the conduit thereby substantially preventing the magnetic sacrificial beads from coming into contact with an immunosensor; contacting the blood sample with the immunosensor to form a sandwich comprising an immobilized capture antibody, a sample analyte and a signal antibody; optionally washing the blood sample from the immunosensor; and detecting the presence of the analyte based on a signal provided by the signal antibody. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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35. A method of performing an analyte competitive immunoassay in a blood sample, comprising:
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introducing a blood sample containing leukocytes into a holding chamber in a test cartridge, wherein a portion of the holding chamber is coated with magnetic sacrificial beads opsonized to leukocytes; amending the blood sample with a labeled analyte; contacting the blood sample with the magnetic sacrificial beads under conditions sufficient to bind at least a portion of the leukocytes in the biological sample to the magnetic sacrificial beads; applying a magnetic field localized to at least a portion of a conduit to attract and retain the magnetic sacrificial beads on at least a portion of a wall of the conduit thereby substantially preventing the magnetic sacrificial beads from coming into contact with an immunosensor; contacting the blood sample with an immunosensor to form a competitive ratio of bound sample analyte to bound labeled analyte; optionally washing the blood sample from the immunosensor; and detecting the presence of the analyte based on the competitive ratio as determined by a signal provided by the bound labeled analyte. - View Dependent Claims (36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53)
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54-71. -71. (canceled)
Specification