MICROFLUIDIC ELEMENT FOR ANALYZING A LIQUID SAMPLE

• US 20120301371A1
• Filed: 06/04/2012
• Published: 11/29/2012
• Est. Priority Date: 12/04/2009
• Status: Active Grant

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Augstein, Manfred, Boehm, Christoph, Effenhauser, Carlo, Oosterbroek, Rijk Edwin, Wuerl, Susanne
• Granted Patent: US 8,911,684 B2
• Time in Patent Office: Days
• US Class Current: 422/502
• CPC Class Codes: B01F 21/22 using additional holders in conduits, containers or pools for keeping the solid material in place, e.g. supports or receptacles
• CPC Class Codes: B01F 29/30 Mixing the contents of individual packages or containers, e.g. by rotating tins or bottles
• CPC Class Codes: B01F 31/10 with a mixing receptacle rotating alternately in opposite directions
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01F 35/712 for feeding fluids
• CPC Class Codes: B01F 35/71725 using centrifugal forces
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence

Abstract

A microfluidic element for analyzing a bodily fluid sample for an analyte contained therein is provided, the element having a substrate, a channel structure that is enclosed by the substrate, and a cover layer, and is rotatable around a rotational axis. The channel structure of the microfluidic element includes a feed channel having a feed opening, a ventilation channel having a ventilation opening, and at least two reagent chambers. The reagent chambers are connected to one another via two connection channels in such a manner that a fluid exchange is possible between the reagent chambers, one of the reagent chambers having an inlet opening, which has a fluid connection to the feed channel, so that a liquid sample can flow into the rotational-axis-distal reagent chamber. At least one of the reagent chambers contains a reagent, which reacts with the liquid sample.

Claims

1. A microfluidic element for analyzing a liquid sample comprising a substrate, a channel structure enclosed by the substrate, and a cover layer, wherein

the microfluidic element is rotatable around a rotational axis;

the channel structure includes a feed channel having a feed opening, a ventilation channel having a ventilation opening, and at least two reagent chambers;

the reagent chambers are connected to one another via two connection channels in such a manner that a fluid exchange is possible between the reagent chambers,

one of the reagent chambers has an inlet opening, which has a fluid connection to the feed channel, so that a liquid sample can flow into the rotational-axis-distal reagent chamber, which, of the two reagent chambers, is positioned farther away from the rotational axis, and

at least one of the reagent chambers contains a reagent, which reacts with the liquid sample.

2. The microfluidic element according to claim 1, wherein the microfluidic element is a test carrier, through which the rotational axis extends.

3. The microfluidic element according to claim 1, wherein the channel structure is an analysis function channel, which comprises a measuring chamber.

4. The microfluidic element according to claim 1, wherein the rotational-axis-distal reagent chamber has the inlet opening.

5. The microfluidic element according to claim 1, wherein the channel structure comprises a mixing chamber, in which the reagent chambers and the connection channels between the reagent chambers are integrated.

6. The microfluidic element according to claim 5, wherein the mixing chamber has a rotational-axis-proximal inlet opening;

and a capillary transport channel is implemented laterally and radially externally on the reagent chambers in the mixing chamber, whose cross section is smaller than the cross section of the connection channels, so that liquid flows through the transport channel from the rotational-axis-proximal inlet opening to the rotational-axis-distal reagent chamber, which is opposite to the inlet opening.

7. The microfluidic element according to claim 1, wherein the reagent chambers are positioned in series in the radial direction in such a manner that the series of the reagent chambers encloses an angle of at most 80°

to the radial direction.

8. The microfluidic element according claim 1, wherein the reagent chambers are essentially hemispherical, the opening surface of the hemisphere being terminated by the cover layer of the microfluidic element.

9. The microfluidic element according to claim 5, wherein webs are implemented between two adjacent reagent chambers in the mixing chamber, and wherein the webs, by which the reagent chambers in the mixing chamber are separated, extend perpendicularly to the cover layer.

10. The microfluidic element according to claim 1, wherein the reagent chamber adjacent to the rotational axis has the inlet opening and an air inlet, which connects the reagent chamber to a ventilation channel.

11. The microfluidic element according to claim 1, wherein one of the connection channels between two adjacent reagent chambers is positioned in such a manner that it aligns with the centers of the two reagent chambers.

12. The microfluidic element according to claim 11, wherein the second connection channel is connected laterally to the two reagent chambers in such a manner that it extends outside a central axis connecting the centers of two adjacent reagent chambers.

13. The microfluidic element according to claim 1, wherein the two adjacent reagent chambers are positioned in such a manner that their spacing is less than the smallest dimension of the reagent chambers in test carrier plane.

14. The microfluidic element according to claim 1, wherein the reagent chambers are implemented so that filling the reagent chamber with a liquid and dissolving of a reagent contained in the reagent chamber occurs without liquid flowing into the adjacent reagent chamber.

15. The microfluidic element according to claim 1, wherein the connection channel has a cross section, in which the smallest cross-sectional dimension is at least 150 μ

m.