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METHOD AND DEVICE FOR DETECTING THE PRESENCE OF A SINGLE TARGET NUCLEIC ACID IN A SAMPLE

  • US 20120301884A1
  • Filed: 08/11/2012
  • Published: 11/29/2012
  • Est. Priority Date: 04/17/1997
  • Status: Active Grant
First Claim
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1. A method for amplifying a target nucleic acid present in a sample, the method comprising:

  • introducing into a common fluidic volume a sample and a nongaseous fluid that is substantially immiscible with the sample, wherein the sample comprises reagents sufficient to perform a primer-based amplification of a target nucleic acid when present;

    segregating the sample into a plurality of reaction volumes ranging from 1 picoliter to 1 microliter, the reaction volumes being segregated and entrapped by the nongaseous fluid in the common fluidic volume; and

    subjecting the plurality of reaction volumes in the common fluidic volume to amplification conditions thereby amplifying the at least one target nucleic acid when present.

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