ACETAMINOPHEN-PROTEIN ADDUCT ASSAY DEVICE AND METHOD

• US 20120301897A1
• Filed: 08/03/2012
• Published: 11/29/2012
• Est. Priority Date: 04/21/2008
• Status: Abandoned Application

• Current Assignee: The Board of Trustees of the University of Arkansas (University of Arkansas System)
• Original Assignee: The Board of Trustees of the University of Arkansas (University of Arkansas System)
• Inventors: James, Laura, Roberts, Dean, Hinson, Jack A.
• Application Number:
  US13/566,516
• Publication Number:
  US 20120301897A1
• Time in Patent Office: Days
• US Class Current: 435/7.9
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/54388 based on lateral flow

Abstract

The present invention describes devices and methods for detecting and measuring the amount of acetaminophen-protein adducts in a sample.

Claims

1. A method of determining an amount of APAP-protein adduct in a sample, comprising:

a. contacting an amount of the sample with a substrate comprising an anti-APAP antibody coupled to an indicator and further comprising a synthetic APAP-protein adduct; and

,

b. determining the amount of APAP-protein adduct in the sample by measuring an indicator change caused by inhibition of binding of the synthetic APAP-protein adduct to the anti-APAP antibody coupled to the indicator.

2. The method of claim 1, wherein the amount of APAP-protein adduct detectible in the sample ranges between about 0.1 nmol/ml of serum and about 100 nmol/ml of serum.

3. The method of claim 2, wherein the amount of APAP-protein adduct detectible in the sample ranges between about 1 nmol/ml of serum and about 40 nmol/ml of serum.

4. The method of claim 1, wherein the sample is selected from the group consisting of blood, urine, saliva, tears, breast milk, lymph, blood plasma, blood serum, bile fluid, cerebrospinal fluid, supernate from cell cultures, tissue extracts, and combinations thereof.

5. The method of claim 1, wherein the sample is conditioned prior to contact with the substrate using methods selected from the group consisting of centrifugation, protein precipitation, fast gel filtration with a molecular weight cutoff of about 5 kDa, and combinations thereof.

6. The method of claim 1, wherein the anti-APAP antibody coupled to an indicator is transported to the synthetic APAP-protein adduct by wicking through the substrate.

7. The method of claim 1, wherein the indicator change is measured using an instrument selected from the group consisting of densitometer, fluorometer, quantitative voltammetry device, and quantitative coulometry device.

8. A method of determining an amount of APAP-protein adduct in a sample, comprising:

a. contacting an amount of the sample with a substrate comprising an amount of a synthetic APAP-protein adduct coupled to an indicator and further comprising an anti-APAP antibody; and

,

b. determining an amount of APAP-protein adduct in a sample by measuring an indicator change caused by inhibition of binding of the anti-APAP antibody to the synthetic APAP-protein adduct coupled to an indicator.

9. The method of claim 8, wherein the amount of APAP-protein adduct detectable in the sample ranges between about 0.1 nmol/ml of serum and about 100 nmol/ml of serum.

10. The method of claim 9, wherein the amount of APAP-protein adduct detectable in the sample ranges between about 1 nmol/ml of serum and about 40 nmol/ml of serum.

11. The method of claim 8, wherein the sample is selected from the group consisting of blood, urine, saliva, tears, breast milk, lymph, blood plasma, blood serum, bile fluid, cerebrospinal fluid, supernate from cell cultures, tissue extracts, and combinations thereof.

12. The method of claim 8, wherein the sample is conditioned prior to contact with the conjugated antibody using methods selected from the group consisting of centrifugation, protein precipitation, fast gel filtration with a molecular weight cutoff of about 5 kDa, and combinations thereof.

13. The method of claim 8, wherein the synthetic APAP-protein adduct is transported to the anti-APAP antibody by wicking through the substrate.

14. The method of claim 8, wherein the indicator change is measured using an instrument selected from the group consisting of densitometer, fluorometer, quantitative voltammetry device, and quantitative coulometry device.

15. A method of determining an amount of an APAP-protein adduct in a sample, comprising:

a. contacting an amount of the sample with a substrate comprising an amount of a first anti-APAP antibody conjugated with an indicator and further comprising a second anti-APAP antibody; and

,

b. determining the amount of APAP-protein adduct in the sample by measuring indicator changes caused by the binding of the APAP-protein adduct to the first anti-APAP antibody and to the second anti-APAP antibody.

16. The method of claim 15, wherein the amount of APAP-protein adduct detectable in the sample ranges between about 0.1 nmol/ml of serum and about 100 nmol/ml of serum.

17. The method of claim 16, wherein the amount of APAP-protein adduct detectable in the sample ranges between about 1 nmol/ml of serum and about 40 nmol/ml of serum.

18. The method of claim 15, wherein the sample is selected from the group consisting of blood, urine, saliva, tears, breast milk, lymph, blood plasma, blood serum, bile fluid, cerebrospinal fluid, supernate from cell cultures, tissue extracts, and combinations thereof.

19. The method of claim 15, wherein the sample is conditioned prior to contact with the conjugated antibody using methods selected from the group consisting of centrifugation, protein precipitation, fast gel filtration with a molecular weight cutoff of about 5 kDa, and combinations thereof.

20. The method of claim 15, wherein the APAP-protein adduct is transported to the first and second anti-APAP antibody by wicking through the substrate.