Method for Determining the Reliability of a Device for Measuring the Concentration of a Substance in Whole Blood, Method for Treating Whole Blood, Container and Kit
First Claim
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1. A method for determining the reliability of a device for measuring the concentration of a substance in whole blood comprising the following successive steps:
- a) mixing a separated, stored, cell-free blood fraction, selected from plasma and serum, and a suspension of blood cells, each separated from whole control blood, with a known amount of the substance being added to the cell-free blood fraction;
b) measuring, by means of the device, the concentration of the substance in the reconstituted whole control blood enriched with said substance in step a);
c) determining the reliability of the device on the basis of the difference between (i) the known concentration of the substance after step a) and (ii) the result of the measurement of the measurement of step b),wherein said cell-free blood fraction and said suspension of blood cells are preferably stored at a temperature of 2-8°
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Abstract
The present invention pertains to the field of measuring the concentration of a substance in whole blood and relates to a method for determining the reliability of a device for measuring the concentration of a substance in whole blood, and to a method for treating whole blood, wherein a cell-free blood fraction enriched with said substance and a suspension of blood cells are stored separately from each other. The invention also relates to a container and to a kit.
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Citations
15 Claims
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1. A method for determining the reliability of a device for measuring the concentration of a substance in whole blood comprising the following successive steps:
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a) mixing a separated, stored, cell-free blood fraction, selected from plasma and serum, and a suspension of blood cells, each separated from whole control blood, with a known amount of the substance being added to the cell-free blood fraction; b) measuring, by means of the device, the concentration of the substance in the reconstituted whole control blood enriched with said substance in step a); c) determining the reliability of the device on the basis of the difference between (i) the known concentration of the substance after step a) and (ii) the result of the measurement of the measurement of step b), wherein said cell-free blood fraction and said suspension of blood cells are preferably stored at a temperature of 2-8°
C. - View Dependent Claims (2, 3, 4, 5, 6, 7, 13, 14, 15)
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8. A method for treating whole blood comprising the following successive steps:
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(a) centrifuging whole blood, with the possible use of one or more additives, so as to obtain a cell-free blood fraction selected from plasma or serum; (b) removing 5-90 volume percent of the cell-free blood fraction, with the possible use of one or more additives, so as to obtain a suspension of blood cells; (c) adding such an amount of a substance to be measured to the cell-free blood fraction that a desired concentration of the substance in the sample is obtained following the reconstitution into a whole blood sample of (i) the cell-free blood fraction enriched with said substance and (ii) an amount of the suspension of blood cells; (d) storing the substance-enriched cell-free blood fraction and the suspension of blood cells separately from each other, wherein preferably 75-85 volume percent of the cell-free blood fraction is removed in step (b), and wherein the cell-free blood fraction and the suspension of blood cells are preferably stored at a temperature of 2-8°
C. - View Dependent Claims (9, 10, 11, 12)
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Specification