BIOPSY INSTRUMENT COMPRISING A MAGNETIC ELEMENT

• US 20120302916A1
• Filed: 10/20/2010
• Published: 11/29/2012
• Est. Priority Date: 10/20/2009
• Status: Active Grant

• Current Assignee: Ogeno GmbH
• Original Assignee: Ogeno GmbH
• Inventors: Pison, Ulrich, Pietschmann, Silvia, Meissner, Wolfgang
• Granted Patent: US 9,247,928 B2
• Time in Patent Office: Days
• US Class Current: 600/573
• CPC Class Codes: A61B 10/02 Instruments for taking cell samples or for biopsy A61B10/0038 and A61B10/0045 take precedence; needle locating or guiding means A61B17/3403; samplers for enzymology or microbiology C12M1/26; sampling or preparing biological specimens G01N33/48
• CPC Class Codes: A61B 10/04 Endoscopic instruments
• CPC Class Codes: A61B 2017/00876 magnetic
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/54346 Nanoparticles
• CPC Class Codes: G01N 33/569 for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

The invention relates to a magnetic biopsy-instrument and use thereof for enriching specific sample material from the body. The invention also relates to a biopsy-kit and use thereof for enriching specific sample material.

Claims

1. A biopsy instrument for the enrichment of sample material, comprising the following components,

i. a guide element comprising a spring-elastic distal portion and a proximal portion,

ii. a magnetic element that is fitted between the distal and the proximal portions of the guide element, and

iii. a stabilizing element connected to the proximal portion of the guide element.

2. The biopsy instrument according to claim 1,

wherein

the components are arranged sequentially from distal to proximal.

3. The biopsy instrument according to claim 1,

wherein

the distal portion is made of metallic or non-metallic materials.

4. The biopsy instrument according to claim 1,

wherein

the stabilizing element is cylindrically.

5. The biopsy instrument according to claim 1,

wherein

the stabilizing element can be slid over the proximal portion of the guide element.

6. The biopsy instrument according to claim 1,

wherein

the outer diameter of the stabilizing element is equal to the outer diameter of the distal portion of the guide element.

7. The biopsy instrument according to claim 1,

wherein

the stabilizing element is reversibly connected to the proximal portion of the guide element.

8. The biopsy instrument according to claim 1,

wherein

the stabilizing element is made of plastic.

9. The biopsy instrument according to claim 1,

wherein

the stabilizing element and the proximal portion of the guide element are mechanically connected, adhesively bonded and/or welded to one another.

10. The biopsy instrument according to claim 1,

wherein

the guide element and the stabilizing element are fabricated using one manufacturing process.

11. The biopsy instrument according to claim 1,

wherein

the magnetic element has a cylindrical shape.

12. The biopsy instrument according to claim 1,

wherein

the magnetic element has an outer diameter that is greater, equal or smaller, preferably 0.01 mm to 0.1 mm smaller, than the outer diameter of the distal portion of the guide element.

13. The biopsy instrument according to claim 1,

wherein

the magnetic element is additionally equipped with detection molecules.

14. The biopsy instrument according to claim 1,

wherein

between the distal and the proximal portions, the guide element has a receptacle device that serves for receiving the magnetic element.

15. The biopsy instrument according to claim 1,

wherein

the magnetic element contains magnetic materials and may comprise metal, plastic and/or ceramic materials, or is an electromagnet.

16. The biopsy instrument according to claim 1,

wherein

the detection molecules are selected from the group comprising antibodies, antibody fragments, protein scaffolds, peptides, nucleic acids, receptors and/or are inorganic materials.

17. The biopsy instrument according to claim 1,

wherein

the spring-elastic distal portion of the guide element comprises detection molecules.

18. The biopsy instrument according to claim 1,

wherein

the biopsy instrument further comprises a covering device.

19. The biopsy instrument according to claim 18,

wherein

the covering device preferably covers the magnetic element, and wherein the covering device comprises homopolymers, copolymers, biopolymers, chemically modified polymers and/or synthetic polymers.

20. The biopsy instrument according to claim 1,

wherein

it has a longitudinal bore for receiving preferably a mandrin and/or other functional elements.

21. A method for binding microorganisms, comprising providing the biopsy instrument according to claim 1, wherein the method comprises:

i. coupling one or more ligands to magnetic nanoparticles;

ii. binding said ligands to microorganisms;

iii. binding the magnetic nanoparticles to at least one magnetic element of the biopsy instrument.

22. The method according to claim 21,

wherein

the one of more ligand is oligoacyllisin and/or a glycopeptide antibiotic.

27. A method comprising:

providing the biopsy instrument of claim 1, wherein the one or more magnetic elements comprise detection molecules and wherein samples are enriched via said biopsy instrument.

29. The method of claim 27, wherein said method is a diagnostic method which includes prenatal diagnosis, cancer diagnosis and therapy follow-up as well as diagnosis of a disease selected from the group consisting of inherited diseases, proliferative diseases, inflammatory diseases, autoimmune diseases, infectious diseases, hormonal disorders, diseases of blood and blood-forming organs, diseases of the digestive tract, liver, bile, pancreas, diseases of the urogenital tract and the kidney, diseases of the heart, abnormal changes in the vascular system and lymphatic system, pulmonary diseases, diseases of the central or peripheral nervous system and of the electrical stimulus transmission, and neurodegenerative diseases.

30. The method of claim 29,

wherein

the prenatal diagnosis refers to a diagnosis of gene mutations, chromosome mutations and chromosome abberations from the group of a deletion, inversion, duplication, translocation and of ring chromosomes, or of a malfunction of gene transcription, gene translation, mRNA stability, of a splice variant, of a malfunction of the mRNA transport into the cytoplasm, of the protein biosynthesis and/or an epigenetic factor;

the cancer diagnosis is used as a primary diagnosis, diagnosis for tumor spread, or for tumor grading;

the therapy follow-up refers to monitoring a tumor treatment, monitoring an autologous, syngeneic, allogeneic, xenogeneic or alloplastic transplantation, monitoring an inflammatory disease, monitoring an infectious disease, monitoring a hormonal disease, monitoring a psychiatric disorder or monitoring a neurodegenerative disease;

the hereditary disease is selected from the group consisting of an autosomal recessive, autosomal dominant, gonosomal and mitochondrial and/or extra-chromosomal hereditary disease, or a disease that can be attributed to a genetic disposition;

the proliferative disease is selected from the group consisting of a tumor, precancerosis, dysplasia, neuroendocrine tumor, endometriosis and metaplasia;

the autoimmune disease is selected from the group consisting of rheumatoid arthritis, inflammatory bowel disease, osteoarthritis, neuropathic pain, alopecia greata, psoriasis, psoriatric arthritis, acute pancreatitis, allograft rejection, allergies, allergic inflammation of the lung, multiple sclerosis, Alzheimer'"'"'s disease, Crohn'"'"'s disease and systemic lupus erythematous;

the infectious disease is selected from the group consisting of parasitic diseases, bacterial diseases or viral diseases;

or the hormonal disease is selected from the group consisting of diseases of sugar metabolism, lipid metabolism, protein metabolism, sexual development and reproduction, water-salt balance, growth and cell formation.

23. A biopsy kit, comprising:

i. a guide element comprising a spring-elastic distal portion and a proximal portion,

ii. one or a plurality of magnetic elements that are fitted between the distal and the proximal portions of the guide element, and additionally comprising detection molecules, and/or

iii. a stabilizing element connected to the proximal portion of the guide element, wherein the guide element, the magnetic element and the stabilizing element are arranged sequentially from distal to proximal.

24. A method for enriching of samples comprising:

providing the biopsy kit of claim 23 and enriching samples from the vascular system, efferent glandular ducts of the pancreas, lachrymal gland, parodit gland, efferent ducts of the mucosal glands, mixed glands, cutaneous and sebaceous glands, mammary gland, spinal canal, brain chamber system, peridural space, gall bladder and its efferent anatomical structures, efferent urinary tracts or the lymphatic system, body cavities of the abdomen or the chest, the uterus, the urogenital apparatus, or a joint or the gastrointestinal tract.

26. A biopsying method comprising:

providing the biopsy kit according to claim 23,

introducing the biopsy kit into a vascular system via a vascular access.

25. A biopsy kit for endoscopic applications, comprising the following components:

i. a guide element comprising a spring-elastic distal portion and a proximal portion,

ii. one or a plurality of magnetic elements that are fitted between the distal and the proximal portions of the guide element,

iii. a stabilizing element connected to the proximal portion of the guide element,

iv. an adaptor and/or

v. a longitudinally movable sheath.

28. (canceled)

31-37. -37. (canceled)