METHOD FOR ACQUIRING GENETICALLY IDENTICAL GAMETE FROM LETHAL FISH HAPLOID-DERIVED GERM CELL VIA GERM LINE CHIMERA
First Claim
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1. A method for acquiring a chimeric fish having a haploid germ cell, said fish being selected from the group consisting of loach, goldfish and zebrafish, comprising the steps of:
- a) genetically inactivating a fish egg or a fish sperm, followed by insemination to provide a haploid donor fertilized egg;
b) obtaining a fertilized egg of a host fish; and
c) transplanting a donor primordial germ cell obtained from a haploid donor embryo developed from the haploid donor fertilized egg into a host embryo derived from the host fertilized egg.
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Abstract
Disclosed are a method for acquiring a germ line chimeric fish having fish haploid germ cells, a germ line chimeric fish having haploid germ cells obtained by the aforesaid method, and a genetically identical gamete, said gamete having been derived from a donor haploid germ cell, produced by a germ line chimeric fish obtained by the aforesaid method.
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Citations
23 Claims
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1. A method for acquiring a chimeric fish having a haploid germ cell, said fish being selected from the group consisting of loach, goldfish and zebrafish, comprising the steps of:
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a) genetically inactivating a fish egg or a fish sperm, followed by insemination to provide a haploid donor fertilized egg; b) obtaining a fertilized egg of a host fish; and c) transplanting a donor primordial germ cell obtained from a haploid donor embryo developed from the haploid donor fertilized egg into a host embryo derived from the host fertilized egg. - View Dependent Claims (5, 6, 7, 8, 9, 13, 14, 15, 16)
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2-4. -4. (canceled)
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10-12. -12. (canceled)
- 17. A chimeric host fish having a haploid primordial germ cell derived from a haploid germ cell.
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20. A haploid clonal fish gamete derived from a haploid primordial germ cell.
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21. (canceled)
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22. A chimeric fish embryo from a host fish embryo transplanted with a haploid donor-derived haploid primordial germ cell.
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23. (canceled)
Specification