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METHOD FOR ACQUIRING GENETICALLY IDENTICAL GAMETE FROM LETHAL FISH HAPLOID-DERIVED GERM CELL VIA GERM LINE CHIMERA

  • US 20120304322A1
  • Filed: 02/09/2011
  • Published: 11/29/2012
  • Est. Priority Date: 02/09/2010
  • Status: Active Grant
First Claim
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1. A method for acquiring a chimeric fish having a haploid germ cell, said fish being selected from the group consisting of loach, goldfish and zebrafish, comprising the steps of:

  • a) genetically inactivating a fish egg or a fish sperm, followed by insemination to provide a haploid donor fertilized egg;

    b) obtaining a fertilized egg of a host fish; and

    c) transplanting a donor primordial germ cell obtained from a haploid donor embryo developed from the haploid donor fertilized egg into a host embryo derived from the host fertilized egg.

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