HERBICIDE TOLERANT SOYBEAN PLANTS AND METHODS FOR IDENTIFYING SAME

• US 20120304331A1
• Filed: 11/23/2010
• Published: 11/29/2012
• Est. Priority Date: 11/23/2009
• Status: Active Grant

• Current Assignee: BASF Agricultural Solutions Seed US LLC (BASF SE), MS Technologies LLC
• Original Assignee: Bayer Bioscience NV (Bayer AG), MS Technologies LLC
• Inventors: Mason, Justin Thomas, Lettow, Leslie James, Eby, Mark Alan, Eby, William H., Welz, Günter, Verhaeghe, Steven, De Beuckeleer, Marc, Habex, Veerle, Ferrulo, Jean-Marc
• Granted Patent: US 9,683,242 B2
• Time in Patent Office: Days
• US Class Current: 800/260
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae

Abstract

The invention provides specific transgenic soybean plants, plant material and seeds, characterized in that these products harbor a stack of specific transformation events at specific locations in the soybean genome. Tools are also provided which allow rapid and unequivocal identification of these events in biological samples.

Claims

1. A method for identifying the simultaneous presence of elite events EE-GM3 and EE-GM1 in biological samples, confirming seed purity in seed samples, or screening seeds for the presence of elite events EE-GM3 and EE-GM1 in samples in seed lots, said method comprises detecting an EE-GM3 specific region with a specific primer or probe which specifically recognizes the 5′

or 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM3 and detecting an EE-GM1 specific region with a specific primer or probe which specifically recognizes the 5′

or 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM1 and identifying the presence or absence of said events in said samples.

2. The method of claim 1, said method comprising amplifying two DNA fragments of between 50 and 1000 bp from a nucleic acid present in said biological samples using a first polymerase chain reaction with at least two primers, one of said primers recognizing the 5′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM3, said 5′

flanking region comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or the 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM3, said 3′

flanking region comprising the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, the other primer of said primers recognizing a sequence within the foreign DNA of EE-GM3 comprising the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No 3 from nucleotide 1 to nucleotide 240 or the other primer of said primers recognizing a sequence within the foreign DNA of EE-GM3 comprising the nucleotide sequence of SEQ ID No. 1 or its complement, or comprising the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or its complement, and using a second polymerase chain reaction with at least two primers, one of said primers recognizing the 5′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM1, said 5′

flanking region comprising the nucleotide sequence of SEQ ID No 12 from nucleotide 1 to nucleotide 209 or the 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM1, said 3′

flanking region comprising the nucleotide sequence of the complement of SEQ ID No 13 from nucleotide 569 to nucleotide 1000, the other primer of said primers recognizing a sequence within the foreign DNA of EE-GM1 comprising the nucleotide sequence of the complement of SEQ ID No. 12 from nucleotide 210 to nucleotide 720 or the nucleotide sequence of SEQ ID No 13 from nucleotide 1 to nucleotide 568 or the other primer of said primers recognizing a sequence within the foreign DNA of EE-GM1 comprising the nucleotide sequence of SEQ ID No.;

11 or its complement, whereby said first and second polymerase reaction may be sequential or simultaneous.

3. The method of claim 2,

(a) wherein said primer recognizing the 5′

flanking region of EE-GM3 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or said primer recognizing the 3′

flanking region of EE-GM3 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and said primer recognizing a sequence within the foreign DNA of EE-GM3 comprises 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No 3 from nucleotide 1 to nucleotide 240 or the nucleotide sequence of SEQ ID No. 1 or its complement, or the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or its complement, and wherein said primer recognizing the 5′

flanking region of EE-GM1 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 12 from nucleotide 1 to nucleotide 209 or said primer recognizing the 3′

flanking region of EE-GM3 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 13 from nucleotide 569 to nucleotide 1000, and said primer recognizing a sequence within the foreign DNA of EE-GM1 comprises 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 12 from nucleotide 210 to nucleotide 720 or the nucleotide sequence of SEQ ID No 13 from nucleotide 1 to nucleotide 568 or the nucleotide sequence of SEQ ID No. 11 or its complement;

or

(b) wherein said primer recognizing the 5′

flanking region of EE-GM3 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or said primer recognizing the 3′

flanking region of EE-GM3 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and said primer recognizing a sequence within the foreign DNA of EE-GM3 comprises at its 3′

end at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No 3 from nucleotide 1 to nucleotide 240 or the nucleotide sequence of SEQ ID No 1 or its complement, or the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or its complement, and wherein said primer recognizing the 5′

flanking region of EE-GM1 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 12 from nucleotide 1 to nucleotide 209 or said primer recognizing the 3′

flanking region of EE-GM1 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 13 from nucleotide 569 to nucleotide 1000, and said primer recognizing a sequence within the foreign DNA of EE-GM1 comprises at its 3′

end at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 12 from nucleotide 210 to nucleotide 720 or the nucleotide sequence of SEQ ID No 13 from nucleotide 1 to nucleotide 568 or the nucleotide sequence of SEQ ID No 11 or its complement.

5. The method of claim 3, wherein said EE-GM3 specific primers comprise the sequence of SEQ ID No. 5 and SEQ ID No. 4, respectively, or the sequence of SEQ ID No 5 and SEQ ID No. 7, respectively, and said EE-GM1 specific primers comprise the sequence of SEQ ID No. 16 and SEQ ID No. 17, respectively.

6. The method of claim 5, which method comprises amplifying a EE-GM3 specific fragment of about 263 or about 706 bp using the EE-GM3 PCR Identification Protocol and a EE-GM1 specific fragment of about 183 bp.

16. The method of claim 1, which method comprises hybridizing a nucleic acid of biological samples with a first specific probe for EE-GM3 and with a second specific probe for EE-GM1.

17. The method of claim 16,

(a) wherein the sequence of said first specific probe has at least 80% sequence identity with a sequence comprising part of the 5′

flanking sequence or the 3′

flanking sequence of EE-GM3 and the sequence of the foreign DNA contiguous therewith and wherein the sequence of said second specific probe has at least 80% sequence identity with a sequence comprising part of the 5′

flanking sequence or the 3′

flanking sequence of EE-GM1 and the sequence of the foreign DNA contiguous therewith;

or

(b) wherein the sequence of said first specific probe has at least 80% sequence identity with SEQ ID No. 2 from nucleotide 1431 to 1472 or SEQ ID No. 3 from nucleotide 220 to 261, or the complement of said sequences and wherein the sequence of said second specific probe has at least 80% sequence identity with SEQ ID No. 12 from nucleotide 199 to 220 or SEQ ID No. 13 from nucleotide 558 to 579, or the complement of said sequences.

4. (canceled)

7. A kit for identifying the simultaneous presence of elite event EE-GM3 and elite event EE-GM1 in biological samples, said kit comprising one primer recognizing the 5′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM3, said 5′

flanking region comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or one primer recognizing the 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM3, said 3′

flanking region comprising the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and one primer recognizing a sequence within the foreign DNA comprising herbicide tolerance genes in EE-GM3, said foreign DNA comprising the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No 3 from nucleotide 1 to nucleotide 240 or the nucleotide sequence of SEQ ID No 1 or its complement, or the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or its complement, and wherein said kit further comprises one primer recognizing the 5′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM1, said 5′

flanking region comprising the nucleotide sequence of SEQ ID No 12 from nucleotide 1 to nucleotide 209 or one primer recognizing the 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM1, said 3′

flanking region comprising the nucleotide sequence of the complement of SEQ ID No 13 from nucleotide 569 to nucleotide 1000, and one primer recognizing a sequence within the foreign DNA comprising herbicide tolerance genes in EE-GM1, said foreign DNA comprising the nucleotide sequence of the complement of SEQ ID No. 12 from nucleotide 210 to nucleotide 720 or the nucleotide sequence of SEQ ID No 13 from nucleotide 1 to nucleotide 568 or the nucleotide sequence of SEQ ID No 11 or its complement.

8. The kit of claim 7,

(a) wherein said primer recognizing said 5′

flanking region of EE-GM3 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or said primer recognizing the 3′

flanking region of EE-GM3 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and said primer recognizing a sequence within the foreign DNA of EE-GM3 comprises 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No 3 from nucleotide 1 to nucleotide 240 or the nucleotide sequence of SEQ ID No 1 or its complement or the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or its complement, and wherein said primer recognizing said 5′

flanking region of EE-GM1 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 12 from nucleotide 1 to nucleotide 209 or said primer recognizing the 3′

flanking region of EE-GM1 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 13 from nucleotide 569 to nucleotide 1000, and said primer recognizing a sequence within the foreign DNA of EE-GM1 comprises 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 12 from nucleotide 210 to nucleotide 720 or the nucleotide sequence of SEQ ID No 13 from nucleotide 1 to nucleotide 568 or the nucleotide sequence of SEQ ID No 11 or its complement;

or

(b) wherein said primer recognizing said 5′

flanking region of EE-GM3 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or said primer recognizing said 3′

flanking region of EE-GM3 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and said primer recognizing a sequence within said foreign DNA of EE-GM3 comprises at its 3′

end at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No 3 from nucleotide 1 to nucleotide 240 or the nucleotide sequence of SEQ ID No 1 or its complement, or the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or its complement, and wherein, said primer recognizing said 5′

flanking region of EE-GM1 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 12 from nucleotide 1 to nucleotide 209 or said primer recognizing said 3′

flanking region of EE-GM1 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 13 from nucleotide 569 to nucleotide 1000, and said primer recognizing a sequence within said foreign DNA of EE-GM1 comprises at its 3′

end at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 12 from nucleotide 210 to nucleotide 720 or the nucleotide sequence of SEQ ID No 13 from nucleotide 1 to nucleotide 568 or the nucleotide sequence of SEQ ID No 11 or its complement;

or

(c) comprising a primer comprising the sequence of SEQ ID No. 4 and a primer comprising the sequence of SEQ ID No. 5 or comprising a primer comprising the sequence of SEQ ID No. 5 and a primer comprising the sequence of SEQ ID No. 7 and further comprising a primer comprising the sequence of SEQ ID No. 16 and a primer comprising the sequence of SEQ ID No. 17.

9. (canceled)

10. (canceled)

11. A pair of primers, said first primer comprising a sequence which, under optimized detection conditions specifically recognizes a sequence within the 5′

or 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM3, said 5′

flanking region comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 and said 3′

flanking region comprising the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and said second primer comprising a sequence which, under optimized detection conditions specifically recognizes a sequence within the 5′

or 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM1, said 5′

flanking region comprising the nucleotide sequence of SEQ ID No 12 from nucleotide 1 to nucleotide 209 and said 3′

flanking region comprising the nucleotide sequence of the complement of SEQ ID No 13 from nucleotide 569 to nucleotide 1000.

12. The pair of primers of claim 11,

(a) wherein said first primer comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408 and wherein said second primer comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 12 from nucleotide 1 to nucleotide 209 or a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 13 from nucleotide 569 to nucleotide 1000;

or

(b) wherein said first primer comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408 and wherein said second primer comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 12 from nucleotide 1 to nucleotide 209 or a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 13 from nucleotide 569 to nucleotide 1000.

13. (canceled)

14. A set of primers,

(a) wherein a first primer comprises at its extreme 3′

end the sequence of SEQ ID No. 5 and a second primer comprises at its extreme 3′

end the sequence of SEQ ID No. 16;

or

(b) wherein a first primer comprises at its extreme 3′

end the sequence of SEQ ID No. 5;

a second primer comprises at its extreme 3′

end the sequence of SEQ ID No. 4;

a third primer comprises at its extreme 3′

end the sequence of SEQ ID No. 16; and

a fourth primer comprises at its extreme 3′

end the sequence of SEQ ID No. 17.

15. (canceled)

18. (canceled)

21. (canceled)

22. A pair of specific probes for the identification of the simultaneous presence of elite event EE-GM3 and elite event EE-GM1 in biological samples, wherein a first specific probe is capable of hybridizing specifically to a specific region of EE-GM3 and a second specific probe is capable of hybridizing specifically to a specific region of EE-GM1.

19. A kit for identifying the simultaneous presence of elite event EE-GM3 and elite event EE-GM1 in biological samples, said kit comprising the first and second specific probes of claim 22.

20. The kit of claim 19,

(a) wherein the sequence of said first specific probe has at least 80% sequence identity with a sequence comprising part of the 5′

flanking sequence or the 3′

flanking sequence of foreign DNA comprising herbicide tolerance genes in EE-GM3 and the sequence of the foreign DNA contiguous therewith and wherein the sequence of said second specific probe has at least 80% sequence identity with a sequence comprising part of the 5′

flanking sequence or the 3′

flanking sequence of foreign DNA comprising herbicide tolerance genes in EE-GM1 and the sequence of the foreign DNA contiguous therewith;

or

(b) wherein the sequence of said first specific probe comprises a nucleotide sequence having at least 80% sequence identity with SEQ ID No. 2 from nucleotide 1441 to 1462 or SEQ ID No. 3 from nucleotide 230 to 251, or the complement of said sequences and wherein the sequence of said second specific probe has at least 80% sequence identity with SEQ ID No. 12 from nucleotide 199 to 220 or SEQ ID No. 13 from nucleotide 558 to 579, or the complement of said sequences.

23. The pair of probes of claim 22,

(a) wherein said first probe comprises a nucleotide sequence having at least 80% sequence identity with a sequence comprising part of the 5′

flanking sequence or the 3′

flanking sequence of foreign DNA comprising herbicide tolerance genes in EE-GM3 and the sequence of the foreign DNA contiguous therewith, or the complement thereof and said second probe comprises a nucleotide sequence having at least 80% sequence identity with a sequence comprising part of the 5′

flanking sequence or the 3′

flanking sequence of foreign DNA comprising herbicide tolerance genes in EE-GM1 and the sequence of the foreign DNA contiguous therewith, or the complement thereof;

or

(b) wherein said first probe has at least 80% sequence identity with SEQ ID No. 2 from nucleotide 1441 to 1462 or SEQ ID No. 3 from nucleotide 230 to 251, or the complement of said sequences, and wherein said second probe has at least 80% sequence identity with SEQ ID No. 12 from nucleotide 199 to 220 or SEQ ID No. 13 from nucleotide 558 to 579, or the complement of said sequences;

or

(c) wherein said first probe comprises a nucleotide sequence being essentially similar to SEQ ID No. 2 from nucleotide 1441 to 1462 or SEQ ID No. 3 from nucleotide 230 to 251, or the complement of said sequences and said second probe comprises a nucleotide sequence being essentially similar to SEQ ID No. 12 from nucleotide 199 to 220 or SEQ ID No. 13 from nucleotide 558 to 579, or the complement of said sequences.

24. (canceled)

25. (canceled)

26. (canceled)

27. (canceled)

28. A method of detecting the presence of elite events EE-GM3 and EE-GM1 in biological samples through hybridization with a substantially complementary labeled nucleic acid probe in which the probe:

target nucleic acid ratio is amplified through recycling of the target nucleic acid sequence, said method comprising;

a) hybridizing said target nucleic acid sequence to a first nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 1452 to nucleotide 1469 or its complement or said first nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID No 3 from nucleotide 223 to nucleotide 240 or its complement;

b) hybridizing said target nucleic acid sequence to a second nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 1434 to nucleotide 1451 or its complement or said labeled nucleic acid probe comprising the nucleotide sequence of SEQ ID No 3 from nucleotide 241 to nucleotide 258 or its complement, wherein said first and second oligonucleotide overlap by at least one nucleotide and wherein either said first or said second oligonucleotide is labeled to be said labeled nucleic acid probe;

c) cleaving only the labeled probe within the probe;

target nucleic acid sequence duplex with an enzyme which causes selective probe cleavage resulting in duplex disassociation, leaving the target sequence intact;

d) recycling of the target nucleic acid sequence by repeating steps (a) to (c); and

e) detecting cleaved labeled probe, thereby determining the presence of said target nucleic acid sequence; and

f) hybridizing said target nucleic acid sequence to a third nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID No 12 from nucleotide 210 to nucleotide 227 or its complement or said third nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID No 13 from nucleotide 561 to nucleotide 568 or its complement;

g) hybridizing said target nucleic acid sequence to a fourth nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID No 12 from nucleotide 192 to nucleotide 209 or its complement or said labeled nucleic acid probe comprising the nucleotide sequence of SEQ ID No 13 from nucleotide 569 to nucleotide 586 or its complement, wherein said third and fourth oligonucleotide overlap by at least one nucleotide and wherein either said third or said fourth oligonucleotide is labeled to be said labeled nucleic acid probe;

h) cleaving only the labeled probe within the probe;

target nucleic acid sequence duplex with an enzyme which causes selective probe cleavage resulting in duplex disassociation, leaving the target sequence intact;

i) recycling of the target nucleic acid sequence by repeating steps (f) to (h); and

j) detecting cleaved labeled probe, thereby determining the presence of said target nucleic acid sequence.

29. A plant, cell, plant part, tissue, seed, or progeny thereof, comprising,

(a) a transgenic soybean plant, or cells, parts, tissue, seed or progeny thereof, each comprising elite event EE-GM3 and elite event EE-GM1 in its genome, reference seed comprising said event EE-GM3 having being deposited at the NCIMB under deposit number NCIMB 41659 and reference seed comprising said event EE-GM1 having being deposited at the NCIMB under deposit number NCIMB 41658, or reference seed comprising elite event EE-GM3 and elite event EE-GM1 in its genome having been deposited at the ATCC under deposit number PTA-11041;

or

(b) a soybean plant, plant part, or seed, cell or tissue thereof, each comprising elite event EE-GM3 and elite event EE-GM1 in its genome, said soybean plant being obtained by crossing a soybean plant comprising elite event EE-GM3 obtainable by propagation of and/or breeding with a soybean plant grown from the seed deposited at the NCIMB under deposit number NCIMB 41659 with a soybean plant comprising elite event EE-GM1 in its genome, obtainable by propagation of and/or breeding with a soybean plant grown from the seed deposited at the NCIMB under deposit number NCIMB 41658, or said soybean plant being obtainable by propagation of and/or breeding with a soybean plant grown from the seed deposited at the ATCC under accession number PTA-11041;

or

(c) a soybean seed comprising elite events EE-GM3 and EE-GM1, reference seed comprising event EE-GM3 having been deposited at the NCIMB under deposit number NCIMB 41659, reference seed comprising event EE-GM1 having been deposited at the NCIMB under deposit number NCIMB 41658, and reference seed comprising events EE-GM3 and EE-GM1 having been deposited at the ATCC under deposit number PTA-11041;

or

(d) a transgenic soybean plant, plant part, cell or tissue, comprising elite events EE-GM3 and EE-GM1, obtainable from the seed of (c).

30. The transgenic soybean plant, seed, cells, tissue, parts or progeny of claim 29, the genomic DNA of which, when analyzed using the Elite event identification protocol for EE-GM3 with two primers comprising the nucleotide sequence of SEQ ID 4 and SEQ ID 5 respectively, yields a DNA fragment of about 263 bp and the genomic DNA of which, when analyzed using the Elite event identification protocol for EE-GM1 with two primers comprising the nucleotide sequence of SEQ ID 16 and SEQ ID 17 respectively, yields a DNA fragment of about 183 bp.

34. A method for producing a soybean plant or seed comprising elite event EE-GM3 and EE-GM1 comprising crossing a plant according to claim 29 with another soybean plant, and planting the seed obtained from said cross.

31. (canceled)

32. (canceled)

33. (canceled)

35. (canceled)

36. A pair of isolated nucleic acid molecules, the first comprising a nucleotide sequence essentially similar to SEQ ID No. 2 from nucleotide 1441 to nucleotide 1452 or SEQ ID No. 3 from nucleotide 230 to 251, or the complement of said sequences and the second comprising a nucleotide sequence essentially similar to SEQ ID No. 12 from nucleotide 199 to nucleotide 220 or SEQ ID No. 13 from nucleotide 558 to 579, or the complement of said sequences.

37. The pair of isolated nucleic acid molecules of claim 36, the first comprising a nucleotide sequence essentially similar to SEQ ID No. 2 from nucleotide 1431 to nucleotide 1462 or SEQ ID No. 3 from nucleotide 220 to 261, or the complement of said sequences and the second comprising a nucleotide sequence essentially similar to SEQ ID No. 12 from nucleotide 189 to nucleotide 230 or SEQ ID No. 13 from nucleotide 548 to 589, or the complement of said sequences

39. A DNA molecule comprising

(i) in order the following nucleotide sequences:

a) the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to 1451;

b) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID 1 from nucleotide 6760 to nucleotide 6958;

c) the nucleotide sequence of SEQ ID 1 from nucleotide 6874 to nucleotide 7298;

d) the nucleotide sequence of SEQ ID 1 from nucleotide 7 to nucleotide 7291;

e) the nucleotide sequence of SEQ ID 1 from nucleotide 12 to nucleotide 7265;

f) the nucleotide sequence of SEQ ID 3 from nucleotide 217 to nucleotide 240;

g) the nucleotide sequence of SEQ ID No 3 from nucleotide 241 to nucleotide 1408; and

further comprising in order the following sequences;

h) the nucleotide sequence of SEQ ID No 12 from nucleotide 1 to 209;

i) the nucleotide sequence of SEQ ID 11 from nucleotide 340 to nucleotide 3461;

j) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID 11 from nucleotide 1 to nucleotide 336;

k) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID 11 from nucleotide 3462 to nucleotide 4076;

l) the nucleotide sequence of SEQ ID 11 from nucleotide 337 to nucleotide 3043;

m) the nucleotide sequence of SEQ ID 13 from nucleotide 559 to nucleotide 568; and

n) the nucleotide sequence of SEQ ID No 13 from nucleotide 569 to nucleotide 1000;

or

(ii) soybean genomic DNA comprising elite event EE-GM3 and EE-GM1.

38. A soybean plant, comprising in its genome the DNA molecule of claim 39 (i) or (ii).

40. A method for identifying the simultaneous presence of elite events EE-GM3 and EE-GM2 in biological samples, confirming seed purity in seed samples, or screening seeds for the presence of elite events EE-GM3 and EE-GM2 in samples in seed lots, which method comprises detection of an EE-GM3 specific region with a specific primer or probe which specifically recognizes the 5′

or 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM3 and detecting an EE-GM2 specific region with a specific primer or probe which specifically recognizes the 5′

or 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM2.

41. The method of claim 40, said method comprising amplifying two DNA fragments of between 50 and 1000 bp from a nucleic acid present in said biological samples using a first polymerase chain reaction with at least two primers, one of said primers recognizing the 5′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM3, said 5′

flanking region comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or the 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM3, said 3′

flanking region comprising the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, the other primer of said primers recognizing a sequence within the foreign DNA of EE-GM3 comprising the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No 3 from nucleotide 1 to nucleotide 240 or the other primer of said primers recognizing a sequence within the foreign DNA of EE-GM3 comprising the nucleotide sequence of SEQ ID No. 1 or its complement, or comprising the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or its complement, and using a second polymerase chain reaction with at least two primers, one of said primers recognizing the 5′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM2, said 5′

flanking region comprising the nucleotide sequence of SEQ ID No 14 from nucleotide 1 to nucleotide 311 or the 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM2, said 3′

flanking region comprising the nucleotide sequence of the complement of SEQ ID No 15 from nucleotide 508 to nucleotide 1880, the other primer of said primers recognizing a sequence within the foreign DNA of EE-GM2 comprising the nucleotide sequence of the complement of SEQ ID No. 14 from nucleotide 312 to nucleotide 810 or the nucleotide sequence of SEQ ID No 15 from nucleotide 1 to nucleotide 507 or the other primer of said primers recognizing a sequence within the foreign DNA of EE-GM2 comprising the nucleotide sequence of SEQ ID No.;

11 or its complement, whereby said first and second polymerase reaction may be sequential or simultaneous.

42. The method of claim 41,

(a) wherein said primer recognizing the 5′

flanking region of EE-GM3 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or said primer recognizing the 3′

flanking region of EE-GM3 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and said primer recognizing a sequence within the foreign DNA of EE-GM3 comprises 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No 3 from nucleotide 1 to nucleotide 240 or the nucleotide sequence of SEQ ID No. 1 or its complement, or the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or its complement, and wherein said primer recognizing the 5′

flanking region of EE-GM2 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 14 from nucleotide 1 to nucleotide 311 or said primer recognizing the 3′

flanking region of EE-GM3 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 15 from nucleotide 508 to nucleotide 1880, and said primer recognizing a sequence within the foreign DNA of EE-GM2 comprises 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 14 from nucleotide 312 to nucleotide 810 or the nucleotide sequence of SEQ ID No 15 from nucleotide 1 to nucleotide 507 or the nucleotide sequence of SEQ ID No. 11 or its complement;

or

(b) wherein said primer recognizing the 5′

flanking region of EE-GM3 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or said primer recognizing the 3′

flanking region of EE-GM3 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and said primer recognizing a sequence within the foreign DNA of EE-GM3 comprises at its 3′

end at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No 3 from nucleotide 1 to nucleotide 240 or the nucleotide sequence of SEQ ID No 1 or its complement, or the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or its complement, and wherein said primer recognizing the 5′

flanking region of EE-GM2 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 14 from nucleotide 1 to nucleotide 311 or said primer recognizing the 3′

flanking region of EE-GM2 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 15 from nucleotide 508 to nucleotide 1880, and said primer recognizing a sequence within the foreign DNA of EE-GM2 comprises at its 3′

end at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 14 from nucleotide 312 to nucleotide 810 or the nucleotide sequence of SEQ ID No 15 from nucleotide 1 to nucleotide 507 or the nucleotide sequence of SEQ ID No 11 or its complement.

55. The method of claim 40, which method comprises hybridizing a nucleic acid of biological samples with a first specific probe for EE-GM3 and with a second specific probe for EE-GM2.

56. The method of claim 55,

(a) wherein the sequence of said first specific probe has at least 80% sequence identity with a sequence comprising part of the 5′

flanking sequence or the 3′

flanking sequence of EE-GM3 and the sequence of the foreign DNA contiguous therewith and wherein the sequence of said second specific probe has at least 80% sequence identity with a sequence comprising part of the 5′

flanking sequence or the 3′

flanking sequence of EE-GM2 and the sequence of the foreign DNA contiguous therewith;

or

(b) wherein the sequence of said first specific probe has at least 80% sequence identity with SEQ ID No. 2 from nucleotide 1431 to 1472 or SEQ ID No. 3 from nucleotide 220 to 261, or the complement of said sequences and wherein the sequence of said second specific probe has at least 80% sequence identity with SEQ ID No. 14 from nucleotide 301 to 322 or SEQ ID No. 15 from nucleotide 497 to 518, or the complement of said sequences.

43. (canceled)

44. The method of claim 43, wherein said EE-GM3 specific primers comprise the sequence of SEQ ID No. 5 and SEQ ID No. 4, respectively, or the sequence of SEQ ID No 7 and SEQ ID No. 5 respectively, and said EE-GM2 specific primers comprise the sequence of SEQ ID No. 18 and SEQ ID No. 19 respectively.

45. The method of claim 44, which method comprises amplifying a EE-GM3 specific fragment of about 263 or 706 bp using the EE-GM3 PCR Identification Protocol and a EE-GM2 specific fragment of about 151 bp.

46. A kit for identifying the simultaneous presence of elite event EE-GM3 and elite event EE-GM2 in biological samples, said kit comprising one primer recognizing the 5′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM3, said 5′

flanking region comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or one primer recognizing the 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM3, said 3′

flanking region comprising the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and one primer recognizing a sequence within the foreign DNA comprising herbicide tolerance genes in EE-GM3, said foreign DNA comprising the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No 3 from nucleotide 1 to nucleotide 240 or the nucleotide sequence of SEQ ID No 1 or its complement, or the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or its complement, and wherein said kit further comprises one primer recognizing the 5′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM2, said 5′

flanking region comprising the nucleotide sequence of SEQ ID No 14 from nucleotide 1 to nucleotide 311 or one primer recognizing the 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM2, said 3′

flanking region comprising the nucleotide sequence of the complement of SEQ ID No 15 from nucleotide 508 to nucleotide 1880, and one primer recognizing a sequence within the foreign DNA comprising herbicide tolerance genes in EE-GM2, said foreign DNA comprising the nucleotide sequence of the complement of SEQ ID No. 14 from nucleotide 312 to nucleotide 810 or the nucleotide sequence of SEQ ID No 15 from nucleotide 1 to nucleotide 507 or the nucleotide sequence of SEQ ID No 11 or its complement.

47. The kit of claim 46,

(a) wherein said primer recognizing said 5′

flanking region of EE-GM3 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or said primer recognizing the 3′

flanking region of EE-GM3 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and said primer recognizing a sequence within the foreign DNA of EE-GM3 comprises 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No 3 from nucleotide 1 to nucleotide 240 or the nucleotide sequence of SEQ ID No 1 or its complement, or the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or its complement, and wherein said primer recognizing said 5′

flanking region of EE-GM2 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 14 from nucleotide 1 to nucleotide 311 or said primer recognizing the 3′

flanking region of EE-GM2 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 15 from nucleotide 508 to nucleotide 1880, and said primer recognizing a sequence within the foreign DNA of EE-GM2 comprises 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 14 from nucleotide 312 to nucleotide 810 or the nucleotide sequence of SEQ ID No 15 from nucleotide 1 to nucleotide 507 or the nucleotide sequence of SEQ ID No 11 or its complement;

or

(b) wherein said primer recognizing said 5′

flanking region of EE-GM3 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or said primer recognizing said 3′

flanking region of EE-GM3 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and said primer recognizing a sequence within said foreign DNA of EE-GM3 comprises at its 3′

end at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No 3 from nucleotide 1 to nucleotide 240 or the nucleotide sequence of SEQ ID No 1 or its complement, or the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or its complement, and wherein, said primer recognizing said 5′

flanking region of EE-GM2 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 14 from nucleotide 1 to nucleotide 311 or said primer recognizing said 3′

flanking region of EE-GM2 comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 15 from nucleotide 508 to nucleotide 1880, and said primer recognizing a sequence within said foreign DNA of EE-GM2 comprises at its 3′

end at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No. 14 from nucleotide 312 to nucleotide 810 or the nucleotide sequence of SEQ ID No 15 from nucleotide 1 to nucleotide 507 or the nucleotide sequence of SEQ ID No 11 or its complement;

or

(c) comprising a primer comprising the sequence of SEQ ID No. 4 and a primer comprising the sequence of SEQ ID No. 5 or comprising a primer comprising the sequence of SEQ ID No. 5 and a primer comprising the sequence of SEQ ID No. 7 and further comprising a primer comprising the sequence of SEQ ID No. 18 and a primer comprising the sequence of SEQ ID No. 19.

48. (canceled)

49. (canceled)

50. A pair of primers suitable for use in an EE-GM3 and EE-GM2 specific detection, said first primer comprising a sequence which, under optimized detection conditions specifically recognizes a sequence within the 5′

or 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM3, said 5′

flanking region comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 and said 3′

flanking region comprising the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408 and said second primer comprising a sequence which, under optimized detection conditions specifically recognizes a sequence within the 5′

or 3′

flanking region of foreign DNA comprising herbicide tolerance genes in EE-GM2, said 5′

flanking region comprising the nucleotide sequence of SEQ ID No 14 from nucleotide 1 to nucleotide 311 and said 3′

flanking region comprising the nucleotide sequence of the complement of SEQ ID No 15 from nucleotide 508 to nucleotide 1880.

51. The pair of primers of claim 50,

(a) wherein said first primer comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408 and wherein said second primer comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 14 from nucleotide 1 to nucleotide 311 or a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 15 from nucleotide 508 to nucleotide 1880;

or

(b) wherein said first primer comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 3 from nucleotide 241 to nucleotide 1408 and wherein said second primer comprises at its extreme 3′

end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID No 14 from nucleotide 1 to nucleotide 311 or a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID No 15 from nucleotide 508 to nucleotide 1880.

52. (canceled)

53. A set of primers,

(a) wherein a first primer comprises at its extreme 3′

end the sequence of SEQ ID No. 5 and wherein a second primer comprises at its extreme 3′

end the sequence of SEQ ID No. 18;

or

(b) wherein a first primer comprises at its extreme 3′

end the sequence of SEQ ID No. 5;

a second primer comprises at its extreme 3′

end the sequence of SEQ ID No. 4;

a third primer comprises at its extreme 3′

end the sequence of SEQ ID No. 18; and

a fourth primer comprises at its extreme 3′

end the sequence of SEQ ID No. 19.

54. (canceled)

57. (canceled)

60. (canceled)

61. A pair of specific probes for the identification of the simultaneous presence of elite event EE-GM3 and elite event EE-GM2 in biological samples wherein a first specific probe is a capable of hybridizing specifically to a specific region of EE-GM3 and a second specific probe capable of hybridizing specifically to a specific region of EE-GM2.

58. A kit for identifying the simultaneous presence of elite event EE-GM3 and elite event EE-GM2 in biological samples, said kit comprising the first and second specific probe of claim 61, capable of hybridizing specifically to a specific region of EE-GM3 and a second specific probe capable of hybridizing specifically to a specific region of EE-GM2.

59. The kit of claim 58,

(a) wherein the sequence of said first specific probe has at least 80% sequence identity with a sequence comprising part of the 5′

flanking sequence or the 3′

flanking sequence of foreign DNA comprising herbicide tolerance genes in EE-GM3 and the sequence of the foreign DNA contiguous therewith and wherein the sequence of said second specific probe has at least 80% sequence identity with a sequence comprising part of the 5′

flanking sequence or the 3′

flanking sequence of foreign DNA comprising herbicide tolerance genes in EE-GM2 and the sequence of the foreign DNA contiguous therewith;

or

(b) wherein the sequence of said first specific probe comprises a nucleotide sequence having at least 80% sequence identity with SEQ ID No. 2 from nucleotide 1441 to 1462 or SEQ ID No. 3 from nucleotide 230 to 251, or the complement of said sequences and wherein the sequence of said second specific probe has at least 80% sequence identity with SEQ ID No. 14 from nucleotide 301 to 322 or SEQ ID No. 15 from nucleotide 497 to 518, or the complement of said sequences.

62. The pair of probes of claim 61,

(a) wherein said first probe comprises a nucleotide sequence having at least 80% sequence identity with a sequence comprising part of the 5′

flanking sequence or the 3′

flanking sequence of foreign DNA comprising herbicide tolerance genes in EE-GM3 and the sequence of the foreign DNA contiguous therewith, or the complement thereof, and said second probe comprises a nucleotide sequence having at least 80% sequence identity with a sequence comprising part of the 5′

flanking sequence or the 3′

flanking sequence of foreign DNA comprising herbicide tolerance genes in EE-GM2 and the sequence of the foreign DNA contiguous therewith, or the complement thereof;

or

(b) wherein said first probe has at least 80% sequence identity with SEQ ID No. 2 from nucleotide 1441 to 1462 or SEQ ID No. 3 from nucleotide 230 to 251, or the complement of said sequences and wherein said second probe has at least 80% sequence identity with SEQ ID No. 14 from nucleotide 301 to 322 or SEQ ID No. 15 from nucleotide 497 to 518, or the complement of said sequences;

or

(c) wherein said first probe comprises a nucleotide sequence being essentially similar to SEQ ID No. 2 from nucleotide 1441 to 1462 or SEQ ID No. 3 from nucleotide 230 to 251, or the complement of said sequences, and said second probe comprises a nucleotide sequence being essentially similar to SEQ ID No. 14 from nucleotide 301 to 322 or SEQ ID No. 15 from nucleotide 497 to 518, or the complement of said sequences.

63. (canceled)

64. (canceled)

65. (canceled)

66. (canceled)

67. A method of detecting the presence of elite events EE-GM3 and EE-GM2 in biological samples through hybridization with a substantially complementary labeled nucleic acid probe in which the probe:

target nucleic acid ratio is amplified through recycling of the target nucleic acid sequence, said method comprising;

a) hybridizing said target nucleic acid sequence to a first nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 1452 to nucleotide 1469 or its complement or said first nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID No 3 from nucleotide 223 to nucleotide 240 or its complement;

b) hybridizing said target nucleic acid sequence to a second nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 1434 to nucleotide 1451 or its complement or said labeled nucleic acid probe comprising the nucleotide sequence of SEQ ID No 3 from nucleotide 241 to nucleotide 258 or its complement, wherein said first and second oligonucleotide overlap by at least one nucleotide and wherein either said first or said second oligonucleotide is labeled to be said labeled nucleic acid probe;

c) cleaving only the labeled probe within the probe;

target nucleic acid sequence duplex with an enzyme which causes selective probe cleavage resulting in duplex disassociation, leaving the target sequence intact;

d) recycling of the target nucleic acid sequence by repeating steps (a) to (c); and

e) detecting cleaved labeled probe, thereby determining the presence of said target nucleic acid sequence, and

f) hybridizing said target nucleic acid sequence to a third nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID No 14 from nucleotide 312 to nucleotide 329 or its complement or said third nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID No 15 from nucleotide 490 to nucleotide 507 or its complement;

g) hybridizing said target nucleic acid sequence to a fourth nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID No 14 from nucleotide 294 to nucleotide 311 or its complement or said labeled nucleic acid probe comprising the nucleotide sequence of SEQ ID No 15 from nucleotide 508 to nucleotide 525 or its complement, wherein said third and fourth oligonucleotide overlap by at least one nucleotide and wherein either said third or said fourth oligonucleotide is labeled to be said labeled nucleic acid probe;

h) cleaving only the labeled probe within the probe;

target nucleic acid sequence duplex with an enzyme which causes selective probe cleavage resulting in duplex disassociation, leaving the target sequence intact;

i) recycling of the target nucleic acid sequence by repeating steps (f) to (h); and

j) detecting cleaved labeled probe, thereby determining the presence of said target nucleic acid sequence.

68. A plant, cell, plant part, tissue, seed, or progeny thereof, comprising,

(a) transgenic soybean plant, or cells, parts, seed or progeny thereof, each comprising elite event EE-GM3 and elite event EE-GM2 in its genome, reference seed comprising said event EE-GM3 having being deposited at the NCIMB under deposit number NCIMB 41659 and reference seed comprising said event EE-GM2 having being deposited at the NCIMB under deposit number NCIMB 41660, or reference seed comprising elite event EE-GM3 and elite event EE-GM2 in its genome having been deposited at the ATCC under deposit number PTA-11042;

or

(b) a soybean plant, or seed, cells or tissues thereof, each comprising elite event EE-GM3 and elite event EE-GM2 in its genome, said soybean plant being obtained by crossing a soybean plant comprising elite event EE-GM3 in its genome, obtainable by propagation of and/or breeding with a soybean plant grown from the seed deposited at the NCIMB under deposit number NCIMB 41659 with a soybean plant comprising elite event EE-GM2 in its genome, obtainable by propagation of and/or breeding with a soybean plant grown from the seed deposited at the NCIMB under deposit number NCIMB 41660, or said soybean plant being obtainable by propagation of and/or breeding with a soybean plant grown from the seed deposited at the ATCC under accession number PTA-11042;

or

(c) a soybean seed comprising elite events EE-GM3 and EE-GM2, reference seed comprising event EE-GM3 having been deposited at the NCIMB under deposit number NCIMB 41659, reference seed comprising event EE-GM2 having been deposited at the NCIMB under deposit number NCIMB 41660, and reference seed comprising events EE-GM3 and EE-GM2 having been deposited at the ATCC under deposit number PTA-11042;

or

(d) a transgenic soybean plant, cell or tissue, comprising elite events EE-GM3 and EE-GM2, produced from the seed of (c);

or

(e) a soybean plant, cell, tissue or seed, comprising EE-GM3 and EE-GM1, comprising in the genome of its cells a nucleic acid sequence with at least 80%, 90%, 95% or 100% sequence identity to SEQ ID No. 2 from nucleotide 1431 to 1472 and a nucleic acid sequence with at least 80%, 90%, 95% or 100% sequence identity to SEQ ID No. 3 from nucleotide 220 to 261, or the complement of said sequences, and also a nucleic acid sequence with at least 80%. 90%, 95% or 100% sequence identity to SEQ ID No. 12 from nucleotide 199 to 220 and a nucleic acid sequence with at least 80%, 90%, 95% or 100% sequence identity to SEQ ID No. 13 from nucleotide 558 to 579, or the complement of said sequences;

or

(f) a soybean plant, cell, tissue or seed, comprising EE-GM3 and EE-GM2, comprising in the genome of its cells a nucleic acid sequence with at least 80%, 90%, 95% or 100% sequence identity to SEQ ID No. 2 from nucleotide 1431 to 1472 and a nucleic acid sequence with at least 80%, 90%, 95% or 100% sequence identity to SEQ ID No. 3 from nucleotide 220 to 261, or the complement of said sequences, and also a nucleic acid sequence with at least 80%, 90%, 95% or 100% sequence identity to SEQ ID No. 14 from nucleotide 301 to 322 and a nucleic acid sequence with at least 80%, 90%, 95% or 100% sequence identity to SEQ ID No. 15 from nucleotide 497 to 518, or the complement of said sequences;

(g) a soybean plant, plant cell, tissue, or seed, comprising in their genome a nucleic acid molecule comprising a nucleotide sequence with at least 97, 98, or at least 99% sequence identity to the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or the complement thereof, or a nucleotide sequence with at least 97, 98, or at least 99% sequence identity to SEQ ID No. 20 or the complement thereof, and comprising in their genome a nucleic acid molecule comprising a nucleotide sequence with at least 97, 98, or at least 99% sequence identity to the nucleotide sequence of EE-GM1 or EE-GM2 or the complement thereof, reference seed comprising EE-GM1 having been deposited under deposit number NCIMB 41658, and reference seed comprising EE-GM2 having been deposited under deposit number NCIMB 41660, such as wherein said nucleotide sequence of EE-GM1 or EE-GM2 comprises or is the foreign DNA sequence in SEQ ID No 12 or 13, or the foreign DNA sequence in SEQ ID No 14 or 15, respectively, or the foreign DNA sequence in the plant genome between the sequence of SEQ ID No 12 and that of SEQ ID No 13, or the foreign DNA sequence in the plant genome between the sequence of SEQ ID No 14 and that of SEQ ID No 15, respectively;

or

(h) a soybean plant, plant cell, tissue, or seed, comprising in their genome a nucleic acid molecule hybridizing to the nucleotide sequence of SEQ ID No 1 or the complement thereof, or hybridizing to the nucleotide sequence of SEQ ID No. 20 from nucleotide position 1452 to nucleotide position 16638 or the complement thereof, or hybridizing to the nucleotide sequence of SEQ ID No. 20 or the complement thereof, and comprising in their genome a nucleic acid molecule hybridizing to the nucleotide sequence of EE-GM1 or EE-GM2 or the complement thereof, reference seed comprising EE-GM1 having been deposited under deposit number NCIMB 41658, and reference seed comprising EE-GM2 having been deposited under deposit number NCIMB 41660, such as wherein said nucleotide sequence of EE-GM1 or EE-GM2 comprises or is the foreign DNA sequence in SEQ ID No 12 or 13, or the foreign DNA sequence in SEQ ID No 14 or 15, or the foreign DNA sequence in the plant genome between the sequence of SEQ ID No 12 and that of SEQ ID No 13, or the foreign DNA sequence in the plant genome between the sequence of SEQ ID No 14 and that of SEQ ID No 15.

69. The transgenic soybean plant, seed, cells, parts or progeny of claim 68, the genomic DNA of which, when analyzed using the Elite event identification protocol for EE-GM3 with two primers comprising the nucleotide sequence of SEQ ID 4 and SEQ ID 5 respectively, yields a DNA fragment of about 263 bp and the genomic DNA of which, when analyzed using the Elite event identification protocol for EE-GM2 with two primers comprising the nucleotide sequence of SEQ ID 18 and SEQ ID 19 respectively, yields a DNA fragment of about 151 bp.

73. A method for producing a soybean plant or seed comprising elite event EE-GM3 and EE-GM2 comprising crossing a plant according to claim 68 with another soybean plant, and planting the seed obtained from said cross.

70. (canceled)

71. (canceled)

72. (canceled)

74. (canceled)

75. A pair of isolated nucleic acid molecules, the first comprising a nucleotide sequence essentially similar to SEQ ID No. 2 from nucleotide 1441 to nucleotide 1452 or SEQ ID No. 3 from nucleotide 230 to 251, or the complement of said sequences, and the second comprising a nucleotide sequence essentially similar to SEQ ID No. 14 from nucleotide 301 to nucleotide 322 or SEQ ID No. 15 from nucleotide 497 to 518, or the complement of said sequences.

76. The pair of isolated nucleic acid molecules of claim 75 the first comprising a nucleotide sequence essentially similar to SEQ ID No. 2 from nucleotide 1431 to nucleotide 1462 or SEQ ID No. 3 from nucleotide 220 to 261, or the complement of said sequences, and the second comprising a nucleotide sequence essentially similar to SEQ ID No. 14 from nucleotide 301 to nucleotide 322 or SEQ ID No. 15 from nucleotide 487 to 518, or the complement of said sequences.

78. A DNA molecule comprising

(i) in order the following nucleotide sequences:

a) the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to 1451;

b) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID 1 from nucleotide 6760 to nucleotide 6958;

c) the nucleotide sequence of SEQ ID 1 from nucleotide 6874 to nucleotide 7298;

d) the nucleotide sequence of SEQ ID 1 from nucleotide 7 to nucleotide 7291;

e) the nucleotide sequence of SEQ ID 1 from nucleotide 12 to nucleotide 7265;

f) the nucleotide sequence of SEQ ID 3 from nucleotide 217 to nucleotide 240; and

g) the nucleotide sequence of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and further comprising in order the following sequences;

h) the nucleotide sequence of SEQ ID No 14 from nucleotide 1 to 311;

i) the nucleotide sequence of SEQ ID 11 from nucleotide 3458 to nucleotide 3848;

j) the nucleotide sequence of SEQ ID 11 from nucleotide 413 to nucleotide 3457;

k) the nucleotide sequence of SEQ ID No 15 from nucleotide 508 to nucleotide 1880;

or

(ii) soybean genomic DNA comprising elite event EE-GM3 and EE-GM2.

77. A soybean plant, comprising in its genome the DNA molecule of claim 78 (i) or (ii).

79. (canceled)

80. (canceled)

81. (canceled)

82. (canceled)