REMOVAL OF VIRULENCE FACTORS THROUGH EXTRACORPOREAL THERAPY
First Claim
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1. A method for removal from mammalians blood of a pathogen or a toxin from said pathogen wherein said pathogen is a member selected from the group consisting of Bacillus anthracis, Pseudomonas aureginosa, and Staphylococcus aureus, said method comprising:
- a. bringing a sample of blood in contact with a carbohydrate immobilized on a solid substrate, said carbohydrate having binding affinity for said pathogen or toxin, under conditions allowing the binding of said substrate to said pathogen or toxin in said sample of blood;
b. separating the sample from said substrate whereby said pathogen or said toxin is at least partially retained or said substrate and the removed sample has a reduced amount of said pathogen or toxin.
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Abstract
A method to remove virulence factors from infected blood by passing the blood through a surface cartridge with immobilized carbohydrates, such as heparin, wherein the virulence factors are toxins released from pathogens such as B. anthracis, S. aureus, and P. aeruginosa.
20 Citations
24 Claims
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1. A method for removal from mammalians blood of a pathogen or a toxin from said pathogen wherein said pathogen is a member selected from the group consisting of Bacillus anthracis, Pseudomonas aureginosa, and Staphylococcus aureus, said method comprising:
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a. bringing a sample of blood in contact with a carbohydrate immobilized on a solid substrate, said carbohydrate having binding affinity for said pathogen or toxin, under conditions allowing the binding of said substrate to said pathogen or toxin in said sample of blood; b. separating the sample from said substrate whereby said pathogen or said toxin is at least partially retained or said substrate and the removed sample has a reduced amount of said pathogen or toxin. - View Dependent Claims (2, 3, 4, 5, 6, 13, 14, 15, 16, 17, 18, 19, 20, 23, 24)
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7. A method for removing from blood at least one pathogen or toxin from said pathogen, wherein said pathogen is a member selected from the group consisting of Bacillus anthracis, Pseudomonas aureginosa, and Staphylococcus aureus, said method comprising:
causing a sample of blood or serum to flow over and past a high-surface-area solid substrate at a flow rate of ≧
50 ml/min wherein the surface of said solid substrate comprises heparin with a binding affinity for the pathogen or toxin, wherein said substrate is sufficiently nonporous such that adsorbents in the blood do not need to pass through pores in said substrate prior to adsorption, and wherein the size of the interstitial channel spaces between individual portions of said substrate and the amount of interstitial substrate surface area is such that when said flowing blood or serum in contact with said substrate at a flow rate of ≧
50 ml/min, said pathogen or toxin binds to said heparin to separate from said blood or serum and the transport of said blood or serum and adsorbents contained in it past said substrate is by means of convection transport more than Brownian diffusion transport.- View Dependent Claims (8, 9, 10, 12)
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11. A method for removing from blood at least one pathogen or toxin from said pathogen, wherein said pathogen is a member selected from the group consisting of Bacillus anthracis, Pseudomonas aureginosa, and Staphylococcus aureus, said method comprising:
causing a sample of blood to flow in contact with rigid polyethylene beads in a container at a flow rate of ≧
50 ml/min, wherein the surface of said beads comprises heparin with a binding affinity for the pathogen or toxin, wherein said beads are sufficiently rigid such that blood does not pass through pores in said substrate, and wherein the size of the interstitial channel spaces between individual ones of said beads and the amount of interstitial surface area of said beads is such that when said blood is in flow contact with said substrate at a flow rate of ≧
50 ml/min, said pathogen or toxin binds to said heparin to separate from said blood and the flow transport of said blood past said substrate is by means of convection transport more than Brownian diffusion transport method.
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21. A method of treating anthrax in a mammal in need of such treatment, comprising:
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a) bringing a sample of blood from a mammal into flow contact with a solid substrate comprising with rigid polyethylene beads in a container at a flow rate of at least about 150 ml/min, wherein the surface of said beads comprises heparin with a binding affinity for Bacillus anthracis or a toxin therefrom, wherein said beads are sufficiently rigid such that blood does not pass through pores in said substrate, and wherein the size of the interstitial channel spaces between individual ones of said beads and the amount of interstitial surface area of said beads is such that when said blood is in flow contact with said substrate at a flow rate of at least about 150 ml/min, said Bacillus anthracis or said toxin binds to said heparin to separate from said blood and the flow transport of said blood past said substrate is by means of convection transport more than Brownian diffusion transport method; b) separating the blood from the solid substrate. - View Dependent Claims (22)
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Specification