POTATO TRANSFORMATION COMPOSITIONS, SYSTEMS, METHODS, MICROORGANISMS, AND PLANTS

• US 20120311734A1
• Filed: 06/04/2012
• Published: 12/06/2012
• Est. Priority Date: 06/04/2011
• Status: Abandoned Application

• Current Assignee: Texas A&M University System
• Original Assignee: Texas A&M University System
• Inventors: Mirkov, T. Erik, Curtis, Ian S., Gonzalez-Ramos, Javier
• Application Number:
  US13/488,120
• Publication Number:
  US 20120311734A1
• Time in Patent Office: Days
• US Class Current: 800/265
• CPC Class Codes: C12N 15/8205 Agrobacterium mediated transformation
• CPC Class Codes: C12N 15/8281 for bacterial resistance

Abstract

The present disclosure relates, in some embodiments, to potato transformation compositions, systems, methods, microorganisms, and plants (e.g., one or more potato chipping varieties). In some embodiments, a method of transforming and/or transfecting a plant (e.g., ‘Atlantic’ potato) may comprise (a) growing an ‘Atlantic’ potato plant (e.g., from a tuber) for from about 3 weeks to about 4 weeks, (b) removing one or more leaf sections (e.g., each section from about 0.5 cm to about 1 cm in its longest dimension) from the plant, (c) cultivating the one or more sections on a callus induction medium comprising zeatin for about 2 days, and/or (d) contacting the one or more sections with Agrobacterium comprising the exogenous nucleic acids under conditions that permit transfer of the exogenous nucleic acid to the one or more sections to produce at least one transformed and/or transfected plant cell.

Claims

1. A method for transforming and/or transfecting at least one cell of ‘

Atlantic’

potato with an exogenous nucleic acid, the method comprising;

growing a ‘

Atlantic’

potato;

removing one or more leaf sections;

cultivating the one or more sections on a callus induction medium; and

contacting the one or more leaf sections with Agrobacterium comprising the exogenous nucleic acids under conditions that permit transfer of the exogenous nucleic acid to the one or more sections to produce at least one transformed and/or transfected plant cell.

2. A method according to claim 1 further comprising cultivating the one or more sections comprising the at least one plant cell on a selection medium.

3. A method according to claim 1 further comprising cultivating the one or more sections comprising the at least one plant cell on a root induction medium.

4. A method according to claim 1 further comprising regenerating a potato plant from the at least one potato plant cell.

5. A method according to claim 1, wherein the exogenous nucleic acid comprises in a 5′

to 3′

direction at least one expression control sequence, at least one coding sequence, and at least one termination sequence.

6. A method according to claim 5, wherein the at least one coding sequence encodes at least one gene product with antimicrobial activity, antiviral activity, and/or insecticidal activity.

7. A method according to claim 5, wherein the at least one coding sequence comprises SoD2, SoD7, gna, variants thereof, and/or combinations thereof.

8. A plant prepared according to the method of claim 4.

9. A plant prepared according to the method of claim 7.

10. A method according to claim 4 further comprising breeding progeny of the plant.

11. A method according to claim 1, wherein the potato is grown from a shoot for about 3 weeks to about 4 weeks.

12. A method according to claim 1, wherein each section is from about 0.5 cm to about 1 cm in its longest dimension from the plant.

13. A method according to claim 1, wherein the callus induction medium comprises a compound selected from the group consisting of zeatin, from about 0.5 mg/L to about 4 mg/L 1-naphthaleneacetic acid, gibberillic acid, and a combination thereof.

14. A method according to claim 1, wherein the callus induction medium comprises from about 0.5 mg/L to about 4 mg/L zeatin, from about 0.1 mg/L to about 4 mg/L 1-naphthaleneacetic acid, and from about 0.01 mg/L to about 2 mg/L gibberillic acid.

15. A method according to claim 1, wherein the cultivating one or more sections comprises cultivating the one or more sections up to about 4 days.

16. A method according to claim 1, wherein the cultivating one or more sections comprises cultivating the one or more sections up to about 2 days.

17. A method for constitutively expressing an exogenous nucleic acid in a plant cell, the method comprising:

cultivating a section of a plant comprising a plant cell on a callus induction medium comprising a cytokinin for up to about 4 days;

contacting an expression cassette or expression vector with the cytosol of the plant cell,

wherein the expression cassette or expression vector comprises (i) the exogenous nucleic acid, (ii) an expression control sequence operable in the plant to drive constitutive expression of the exogenous nucleic acid, and (iii) a 3′

termination sequence operably linked to the exogenous nucleic acid,

wherein the plant is the Atlantic cultivar of potato, and

wherein the exogenous nucleic acid is expressed.

18. A method according to claim 17, wherein the cytokinin comprises zeatin.

19. A method according to claim 17, wherein the section of the plant is cultivated for about 2 days.

20. A method according to claim 17, wherein the section of the plant is from about 3 weeks to about 4 weeks old at the time of cultivating on the callus induction medium.

21. A method according to claim 17, wherein the exogenous nucleic acid encodes at least one gene product with antimicrobial activity, antiviral activity, and/or insecticidal activity.

22. A method according to claim 21, wherein the at least one gene product comprises SoD2, SoD7, gna, variants thereof, and/or combinations thereof.

23. A method according to claim 17, wherein the contacting further comprises biolistically bombarding the plant with a particle comprising the expression cassette or expression vector.

24. A method according to claim 17, wherein the contacting further comprises co-cultivating the plant with an Agrobacterium cell comprising the expression cassette or expression vector.

25. A method according to claim 17, wherein the contacting further comprises contacting an embryonic callus of the plant with the expression cassette or expression vector.

26. A method according to claim 17 further comprising regenerating a transgenic plant from the plant cell.

27. A method according to claim 26, wherein the regenerating further comprises cultivating the section of the plant on a selection medium and/or a rooting medium.

28. A transgenic plant regenerated according to claim 26.

29. A method according to claim 26 further comprising breeding progeny of the transgenic plant.

30. A method according to claim 22 further comprising regenerating a transgenic plant from the plant cell.

31. A method according to claim 30, wherein the regenerating further comprises cultivating the section of the plant on a selection medium and/or a rooting medium.

32. A transgenic plant regenerated according to claim 30.

33. A method according to claim 30 further comprising breeding progeny of the transgenic plant.

34. An ‘

Atlantic’

potato food product comprising at least one cell of ‘

Atlantic’

potato with an exogenous nucleic acid.

35. An ‘

Atlantic’

potato food product according to claim 34, wherein the food product is a potato chip.

36. An ‘

Atlantic’

potato food product according to claim 34, wherein the food product is a powder, a flour, a flake, a string. a baked potato, a French fry, a hash brown, a pancake, a dumpling, a sauce, or a scone.

37. An ‘

Atlantic’

potato food product prepared from an ‘

Atlantic’

potato comprising at least one exogenous nucleic acid

38. An ‘

Atlantic’

potato food product according to claim 37, wherein the food product is a potato chip.

39. An ‘

Atlantic’

potato food product according to claim 37, wherein the food product is a powder, a flour, a flake, a string. a baked potato, a French fry, a hash brown, a pancake, a dumpling, a sauce, or a scone.