SYSTEM AND METHOD FOR SUB-SURFACE FLUORESCENCE IMAGING
First Claim
1. A system for sub-surface fluorescence topographical imaging comprising:
- an excitation source for selectably emitting light at at least one of at least two excitation wavelengths or wavelength ranges at a target surface; and
a light detector for detecting fluorescence emission wavelengths or wavelength ranges from the target surface;
wherein at least one of the at least two excitation wavelengths or wavelength ranges causes fluorescing of at least one marker at a sub-surface depth, the emitted light at each of the at least two excitation wavelengths or wavelength ranges having different depths of optical penetration and causing fluorescing at respective different depths.
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Accused Products
Abstract
A system for sub-surface fluorescence imaging is provided, the system comprising: an excitation source for selectably emitting light at at least one of at least two excitation wavelengths or wavelength ranges at a target surface; and a light detector for detecting fluorescence emission wavelengths or wavelength ranges from the target surface; wherein at least one of the at least two excitation wavelengths or wavelength ranges causes fluorescing of at least one marker at a sub-surface depth, the emitted light at each of the at least two excitation wavelengths or wavelength ranges having different depths of optical penetration and causing fluorescing at respective different depths. A method for sub-surface fluorescence imaging is also provided, in some cases exemplified by a reconstruction of the sub-surface fluorescence topography.
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Citations
59 Claims
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1. A system for sub-surface fluorescence topographical imaging comprising:
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an excitation source for selectably emitting light at at least one of at least two excitation wavelengths or wavelength ranges at a target surface; and a light detector for detecting fluorescence emission wavelengths or wavelength ranges from the target surface; wherein at least one of the at least two excitation wavelengths or wavelength ranges causes fluorescing of at least one marker at a sub-surface depth, the emitted light at each of the at least two excitation wavelengths or wavelength ranges having different depths of optical penetration and causing fluorescing at respective different depths. - View Dependent Claims (2, 3, 5, 9, 15, 20, 23, 24, 25, 27)
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30. A method for sub-surface fluorescence imaging comprising:
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illuminating a target surface with light at at least two excitation wavelengths or wavelength ranges; wherein at least one of the at least two excitation wavelengths or wavelength ranges causes fluorescing of at least one marker at a sub-surface depth, the light at each of the at least two excitation wavelengths or wavelength ranges having different depths of optical penetration and causing fluorescing at respective different depths; detecting fluorescence emission wavelengths or wavelength ranges from the target surface; determining a fluorescence calculation for each of the at least two excitation wavelengths or wavelength ranges using the detected fluorescence emission wavelengths or wavelength ranges; and constructing a sub-surface fluorescence topographical image using the determined fluorescence calculations. - View Dependent Claims (31, 33, 34, 35, 36, 37, 38, 39, 40, 53)
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Specification