METHOD AND SYSTEM FOR TRANSFERRING AND/OR CONCENTRATING A SAMPLE

• US 20130000738A1
• Filed: 02/28/2012
• Published: 01/03/2013
• Est. Priority Date: 02/28/2011
• Status: Abandoned Application

• Current Assignee: PathoGenetix, Inc. (Toxic Reports LLC)
• Original Assignee: PathoGenetix, Inc. (Toxic Reports LLC)
• Inventors: Krogmeier, Jeffrey R., Larson, Jonathan W., Meltzer, Robert H.
• Application Number:
  US13/406,783
• Publication Number:
  US 20130000738A1
• Time in Patent Office: Days
• US Class Current: 137/3
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/0883 Serpentine channels
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2300/1894 Cooling means; Cryo cooling
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows
• CPC Class Codes: F17D 3/00 Arrangements for supervising or controlling working operations
• CPC Class Codes: Y10T 137/0329 Mixing of plural fluids of diverse characteristics or conditions

Abstract

A method of controlling the concentration of a sample flowing through a microchannel is provided. The method includes flowing a sample carrier fluid from a sample carrier channel and into an upstream portion of a microchannel such that the sample carrier fluid flows from the upstream portion and toward a downstream portion of the microchannel, and flowing a sheathing fluid from a sheathing fluid channel and into the upstream portion of the microchannel such that the sheathing fluid flows from the upstream portion and toward the downstream portion of the microchannel, and generally parallel to the sample carrier fluid. The method further includes detecting a concentration of a sample in the sample carrier fluid in the downstream portion of the microchannel, and adjusting a flow rate of at least one of the sample carrier fluid and the sheathing fluid based upon the detected concentration of the sample.

Claims

1. A method of controlling the concentration of a sample flowing through a microchannel, the method comprising:

flowing a sample carrier fluid from a sample carrier channel and into an upstream portion of a microchannel such that the sample carrier fluid flows from the upstream portion and toward a downstream portion of the microchannel;

flowing a sheathing fluid from a sheathing fluid channel and into the upstream portion of the microchannel such that the sheathing fluid flows from the upstream portion and toward the downstream portion of the microchannel, and generally parallel to the sample carrier fluid;

detecting a concentration of a sample in the sample carrier fluid in the downstream portion of the microchannel;

adjusting a flow rate of at least one of the sample carrier fluid and the sheathing fluid based upon the detected concentration of the sample.

2. The method of claim 1, wherein flowing a sheathing fluid includes flowing sheathing fluid from both a first sheathing fluid channel and a second sheathing fluid channel, wherein the first sheathing fluid channel is positioned on a first side of the sample fluid channel and the second sheathing fluid channel is positioned on a second side of the sample fluid channel.

3. The method of claim 1, wherein adjusting a flow rate includes increasing the flow rate of the sample carrier fluid to increase the concentration of the sample.

4. The method of claim 1, wherein adjusting a flow rate includes decreasing the flow rate of the sheathing fluid to increase the concentration of the sample.

5. The method of claim 1, wherein adjusting a flow rate includes decreasing the flow rate of the sample carrier fluid to decrease the concentration of the sample.

6. The method of claim 1, wherein adjusting a flow rate includes increasing the flow rate of the sheathing fluid to decrease the concentration of the sample.

7. The method of claim 1, wherein adjusting a flow rate of at least one of the sample carrier fluid and the sheathing fluid does not affect the combined flow rate of both the sample carrier fluid and the sheathing fluid.