Methods for High Fidelity Production of Long Nucleic Acid Molecules with Error Control
First Claim
Patent Images
1. A method for generating a nucleic acid molecule with precise user control, the method comprising:
- a) providing a nucleic acid immobilized on a surface;
b) providing a nucleic acid molecule attached to a protecting group;
c) hybridizing said immobilized nucleic acid with said nucleic acid molecule attached to a protecting group, thereby elongating said immobilized nucleic acid;
d) dissociating said elongated immobilized nucleic acid from said protecting group; and
e) repeating steps (b) to (d) until the elongated immobilized nucleic acid has a desired sequence and length.
1 Assignment
0 Petitions
Accused Products
Abstract
A method for synthesizing a nucleic acid having a desired sequence and length comprises providing a solid support having an immobilized nucleic acid, performing a nucleic acid addition reaction to elongate the immobilized nucleic acid by adding a nucleotide or an oligonucleotide attached to a protecting group to the nucleic acid, determining whether the nucleotide or the oligonucleotide is added to the nucleic acid, removing the protecting group, and continuing until the immobilized nucleic acid has a desired sequence and length.
54 Citations
6 Claims
-
1. A method for generating a nucleic acid molecule with precise user control, the method comprising:
-
a) providing a nucleic acid immobilized on a surface; b) providing a nucleic acid molecule attached to a protecting group; c) hybridizing said immobilized nucleic acid with said nucleic acid molecule attached to a protecting group, thereby elongating said immobilized nucleic acid; d) dissociating said elongated immobilized nucleic acid from said protecting group; and e) repeating steps (b) to (d) until the elongated immobilized nucleic acid has a desired sequence and length. - View Dependent Claims (2, 3, 4, 5, 6)
-
Specification