PROCESS FOR PREPARING AZACYTIDINE INTERMEDIATE

• US 20130008238A1
• Filed: 09/14/2012
• Published: 01/10/2013
• Est. Priority Date: 08/06/2008
• Status: Abandoned Application

• Current Assignee: Andrea Giolito, Ettore Bigatti, Giovanna Lux, Maurizio Paiocchi, Simone Tosi
• Original Assignee: Andrea Giolito, Ettore Bigatti, Giovanna Lux, Maurizio Paiocchi, Simone Tosi
• Inventors: BIGATTI, Ettore, Lux, Giovanna, Paiocchi, Maurizio, Giolito, Andrea, Tosi, Simone
• Application Number:
  US13/616,913
• Publication Number:
  US 20130008238A1
• Time in Patent Office: Days
• US Class Current: 73/61.52
• CPC Class Codes: C07H 1/00 Processes for the preparation of sugar derivatives
• CPC Class Codes: C07H 19/12 Triazine radicals
• CPC Class Codes: Y10T 436/147777 Plural nitrogen in the same ring [e.g., barbituates, creatinine, etc.]

Abstract

The present invention provides a processes for preparing 5-Azacytidine, and intermediates thereof. The present invention further provides an analytical method for determining the purity of 5-Azacytidine in a sample.

Claims

1-19. -19. (canceled)

20. A method for determining the purity of 5-azacytidine comprising:

a) providing a sample of 5-Azacytidine,

b) dissolving a first part of the sample in DMSO, and a second part of the sample in DMPU, providing a first and second sample solution,

c) injecting each sample solution onto an HPLC column under the same conditions, providing a first and second chromatogram, and

d) determining the purity of Azacytidine and the amount of each impurity based on both chromatograms.