METHOD FOR OPERATING A MEASUREMENT FOR A SAMPLE ON AN ELECTROCHEMICAL TEST STRIP

• US 20130008802A1
• Filed: 10/04/2011
• Published: 01/10/2013
• Est. Priority Date: 07/06/2011
• Status: Active Grant

• Current Assignee: Bionime Corporation
• Original Assignee: Bionime Corporation
• Inventors: Chen, Chieh-Hsing, Hsu, Cheng-Teng, Su, Chun-Wei
• Granted Patent: US 8,702,960 B2
• Time in Patent Office: Days
• US Class Current: 205/777.5
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771

Abstract

A method for operating a measurement for a sample on an electrochemical test strip including at least two electrodes is provided. The method includes steps of applying a first voltage between the two electrodes during an interference-removal period after an incubation period succeeding a moment when the sample is detected, and applying a second voltage between the two electrodes during a test period, wherein the first voltage is larger than the second voltage, the first voltage includes one of a first fixed voltage and a first set of plural pulse voltages, and the second voltage includes a second fixed voltage.

Claims

1. A method for operating a measurement for a sample on an electrochemical test strip including at least two electrodes, the method comprising steps of:

applying a first voltage between the two electrodes during an interference-removal period after an incubation period succeeding a moment when the sample is detected; and

applying a second voltage between the two electrodes during a test period, wherein the first voltage is larger than the second voltage, the first voltage includes one of a first fixed voltage and a first set of plural pulse voltages, and the second voltage includes a second fixed voltage.

2. A method of claim 1, further comprising a step of applying a detection voltage between the two electrodes during a sample presence detection period before the step of applying the first voltage, wherein:

the step of applying the detection voltage continues till the sample is detected;

when a detected electrical current between the two electrodes is higher than a threshold, the sample is deemed as been detected, and the sample presence detection period is ended;

the detection voltage includes a detection set of plural pulse voltages, each of which is in a range of 0.1-0.5 volt;

each pulse of the detection set of plural pulse voltages has a detection pulse width in a range of 100-600 μ

s; and

the detection set of plural pulse voltages has a detection pulse interval between two adjacent pulses therein, wherein the detection pulse interval is in a range of 10-50 ms.

3. A method of claim 2, wherein:

the sample includes a blood sample;

the each voltage of the detection set of plural pulse voltages is in a range of 0.15-0.25 volt;

the detection pulse width is in a range of 300-400 μ

s; and

the detection pulse interval is in a range of 15-25 ms.

4. A method of claim 1, wherein the electrochemical test strip includes a hydrogen peroxide and a reagent containing a mediator having a reduction state and an oxidation state, and the method further comprises a step of:

during the incubation period, applying an incubation voltage, which is high enough to oxidize the mediator from the reduction state to the oxidation state and is low enough to avoid oxidizing the hydrogen peroxide, wherein;

the incubation voltage includes an incubation set of plural pulse voltages, each of which is in a range of 0.1-0.6 volt;

each pulse of the incubation set of plural pulse voltages has an incubation pulse width in a range of 100-500 μ

s; and

the incubation set of plural pulse voltages has an incubation pulse interval between two adjacent pulses therein, wherein the incubation pulse interval is in a range of 100-500 ms.

5. A method of claim 4, wherein:

the each voltage of the incubation set of plural pulse voltages is in a range of 0.1.5-0.25 volt;

the incubation pulse width is in a range of 150-250 μ

s;

the incubation pulse interval is in a range of 150-250 ms;

the reagent further includes a glucose oxidase enzyme; and

the mediator includes a potassium ferrocyanide.

6. A method of claim 1, wherein one of following operations is performed during the incubation period:

causing a power-off to result in an open-circuit state in a measuring circuit; and

applying a zero voltage between the two electrodes.

7. A method of claim 1, wherein:

the first voltage is in a range of 0.15-1 volt;

the second voltage is in a range of 0.1-0.9 volt;

the step of applying the first voltage is performed for a first applying period in a range of 0.1-1 s; and

the step of applying the second voltage is performed for a second applying period in a range of 0.2-10 s.

8. A method of claim 7, wherein:

the first voltage is in a range of 0.3-0.4 volt;

the second voltage is in a range of 0.15-0.25 volt;

the first applying period is in a range of 0.4-0.6 s;

the second applying period is in a range of 1-2 s; and

the step of applying the second voltage is performed to measure a blood sugar concentration of the sample.

9. A method of claim 1, further comprising a step of:

during a transition interval between the interference-removal period and the test period, wherein the transition interval lasts for 0.01-0.5 s, performing one of following operations;

causing a power-off to result in an open-circuit state in a measuring circuit; and

applying a zero voltage between the two electrodes.

10. A method for operating a measurement for a sample on an electrochemical test strip, wherein the sample includes an interference material and an analyte concentration, the method comprising steps of:

providing the electrochemical test strip;

oxidizing the interference material after a first time period succeeding a moment when the electrochemical test strip is detected to have the sample thereon; and

measuring the analyte concentration.

11. A method of claim 10, wherein the electrochemical test strip includes at least two electrodes, and the method further comprises a step of:

applying a detection voltage between the two electrodes during a sample presence detection period before the step of oxidizing the interference material, wherein;

the step of applying the detection voltage continues till the sample is detected;

when a detected electrical current between the two electrodes is higher than a threshold, the electrochemical test strip is deemed to have the sample thereon, and the sample presence detection period is ended;

the detection voltage includes a detection set of plural pulse voltages, each of which is in a range of 0.1-0.5 volt;

each pulse of the detection set of plural pulse voltages has a detection pulse width in a range of 100-600 μ

s; and

the detection set of plural pulse voltages has a detection pulse interval between two adjacent pulses therein, wherein the detection pulse interval is in a range of 10-50 ms.

13. A method of claim 11, wherein the electrochemical test strip includes a hydrogen peroxide and a reagent containing a mediator having a reduction state and an oxidation state, and the method further comprises a step of:

applying an incubation voltage after the sample presence detection period, wherein the incubation voltage is high enough to oxidize the mediator from the reduction state to the oxidation state and is low enough to avoid oxidizing the hydrogen peroxide, wherein;

the incubation voltage includes an incubation set of plural pulse voltages, each of which is in a range of 0.1-0.6 volt;

each pulse of the incubation set of plural pulse voltages has an incubation pulse width in a range of 100-500 μ

s; and

the incubation set of plural pulse voltages has an incubation pulse interval between two adjacent pulses therein, wherein the incubation pulse interval is in a range of 100-500 ms.

15. A method of claim 10, wherein one of following operations is performed during the incubation period:

causing a power-off to result in an open-circuit state in a measuring circuit; and

applying a zero voltage between the two electrodes.

16. A method of claim 10, wherein:

the sample includes a blood sample;

the electrochemical test strip includes at least two electrodes;

the step of oxidizing the interference material includes applying a first voltage between the two electrodes;

the step of measuring the analyte concentration includes applying a second voltage between the two electrodes to measure an electrical parameter;

the first voltage is higher than the second voltage;

the first voltage includes one of a first fixed voltage and a first set of plural pulse voltages; and

the second voltage includes a second fixed voltage.

17. A method of claim 16, wherein:

the first voltage is in a range of 0.15-1 volt;

the second voltage is in a range of 0.1-0.9 volt;

the step of applying the first voltage is performed for 0.1-1 s; and

the step of applying the second voltage is performed for 0.2-10 s.

18. A method of claim 16, wherein:

the electrical parameter includes a detected electrical current between the two electrodes;

the detected electrical current and the analyte concentration have a mathematical linear relationship in a measuring domain;

the analyte concentration is calculated based on the detected electrical current; and

the analyte concentration includes a blood sugar concentration.

19. A method of claim 10, further comprising a step of:

during a transition interval between the steps of oxidizing the interference material and measuring the analyte concentration, wherein the transition interval lasts 0.01-0.5 s, performing one of following operations;

causing a power-off to result in an open-circuit state in a measuring circuit; and

applying a zero voltage between the two electrodes.

12. A method of claim 12, wherein:

the sample includes a blood sample;

the each voltage of the detection set of plural pulse voltages is in a range of 0.15-0.25 volt;

the detection pulse width is in a range of 300-400 μ

s; and

the detection pulse interval is in a range of 15-25 ms.

14. A method of claim 14, wherein:

the each voltage of the incubation set of plural pulse voltages is in a range of 0.15-0.25 volt;

the incubation pulse width is in a range of 150-250 μ

s;

the incubation pulse interval is in a range of 150-250 ms;

the reagent further includes a glucose oxidase enzyme; and

the mediator includes a potassium ferrocyanide.

20. A method for operating a measurement for a sample on an electrochemical test strip including at least two electrodes, comprising steps of:

treating the sample by a first voltage; and

measuring an electrical parameter of the sample by a second voltage lower than the first voltage.