SAMPLE OBSERVATION APPARATUS
First Claim
1. A sample observation apparatus comprising:
- an excitation light irradiation unit to irradiate a sample with an excitation light;
an excitation light modulation unit to modulate a spatial intensity distribution of the excitation light on the sample;
an excitation light modulation control unit to control the excitation light modulation unit according to a modulation control signal;
a photo detection unit to detect light emission from the sample caused by irradiation with the excitation light to generate a detection signal;
an image generation unit to generate image data of the sample according to the modulation control signal and the detection signal;
a modulation control signal generation unit to generate the modulation control signal such that a Nyquist frequency of the image data will be larger than a cut-off frequency in the spatial intensity distribution of the excitation light on the sample; and
an image processing unit to emphasize a high-frequency component that exceeds the cut-off frequency included in the image data.
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Accused Products
Abstract
A sample observation apparatus includes excitation light irradiation unit to irradiate sample with excitation light; excitation light modulator to modulate spatial intensity distribution of the excitation light on the sample; excitation light modulation control unit to control the excitation light modulator according to modulation control signal; photo detection unit to detect light emission from the sample and to generate a detection signal; image generation unit to generate image data of the sample according to the modulation control signal and the detection signal; modulation control signal generation unit to generate the modulation control signal such that Nyquist frequency of the image data will be larger than cut-off frequency in the spatial intensity distribution of the excitation light on the sample; and image processing unit to emphasize high-frequency component that exceeds the cut-off frequency included in the image data.
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Citations
20 Claims
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1. A sample observation apparatus comprising:
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an excitation light irradiation unit to irradiate a sample with an excitation light; an excitation light modulation unit to modulate a spatial intensity distribution of the excitation light on the sample; an excitation light modulation control unit to control the excitation light modulation unit according to a modulation control signal; a photo detection unit to detect light emission from the sample caused by irradiation with the excitation light to generate a detection signal; an image generation unit to generate image data of the sample according to the modulation control signal and the detection signal; a modulation control signal generation unit to generate the modulation control signal such that a Nyquist frequency of the image data will be larger than a cut-off frequency in the spatial intensity distribution of the excitation light on the sample; and an image processing unit to emphasize a high-frequency component that exceeds the cut-off frequency included in the image data. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification