DEVICES, SYSTEMS, AND METHODS FOR MAGNETIC SEPARATION
First Claim
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1. A method comprising:
- providing a fluid containing a plurality of particles having magnetic labels in a microfluidic device, wherein the microfluidic device includes;
a first substrate at least partially defining a fluidic feature having an opening;
a second substrate reversibly coupled to the opening;
separating the plurality of particles having magnetic labels from the fluid at least in part by generating a magnetic field across the microfluidic device such that the plurality of particles having magnetic labels are driven toward the second substrate; and
removing the second substrate including the plurality of particles having magnetic labels from the opening.
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Abstract
Methods, microfluidic devices, and instruments for magnetic separation of particles from a fluid are described. Examples include microfluidic devices having a removable portion. Examples include microfluidic devices having one or more regions of reduced fluid velocity. Examples further including instruments having pneumatic interfaces. Examples further includes instruments having controllable magnets, imaging components, or combinations thereof.
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Citations
44 Claims
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1. A method comprising:
providing a fluid containing a plurality of particles having magnetic labels in a microfluidic device, wherein the microfluidic device includes; a first substrate at least partially defining a fluidic feature having an opening; a second substrate reversibly coupled to the opening; separating the plurality of particles having magnetic labels from the fluid at least in part by generating a magnetic field across the microfluidic device such that the plurality of particles having magnetic labels are driven toward the second substrate; and removing the second substrate including the plurality of particles having magnetic labels from the opening. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A microfluidic device comprising:
a substrate at least in part defining; an inlet port; a first channel in fluid communication with the inlet port, wherein the first channel has first cross-sectional dimensions; a fluidic feature in fluid communication with the first channel, wherein the fluidic feature has second cross-sectional dimensions, wherein at least one of the second cross-sectional dimensions is larger than the first cross-sectional dimensions; and a frame coupled to a surface of the substrate, wherein the frame at least in part supports a well structure in fluid communication with the inlet port. - View Dependent Claims (15, 16, 17, 18, 19, 20, 33, 34)
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21-32. -32. (canceled)
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35. An apparatus comprising:
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a platform configured to receive a microfluidic device, wherein the microfluidic device includes; a substrate at least in part defining; an inlet port; a first channel in fluid communication with the inlet port, wherein the first channel has first cross-sectional dimensions; and a fluidic feature in fluid communication with the first channel, wherein the fluidic feature has second cross-sectional dimensions, wherein at least one of the second cross-sectional dimensions is larger than the first cross-sectional dimensions; a well structure in fluid communication with the inlet port; a cover configured to seal the well structure, wherein the cover includes a pneumatic interface to the well structure; and a magnet positioned to generate a magnetic field across the fluidic feature. - View Dependent Claims (36, 37, 38, 39, 40, 41, 42, 43, 44)
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Specification