SYSTEMS AND METHODS OF REPLACING INTESTINAL FLORA

• US 20130022575A1
• Filed: 07/19/2011
• Published: 01/24/2013
• Est. Priority Date: 07/19/2011
• Status: Abandoned Application

• Current Assignee: Microbial Rx
• Original Assignee: Microbial Rx
• Inventors: Cassity, Timothy
• Application Number:
  US13/186,274
• Publication Number:
  US 20130022575A1
• Time in Patent Office: Days
• US Class Current: 424/93.3
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/741 Probiotics probiotic yeast, e.g. saccharomyces A61K36/06
• CPC Class Codes: A61K 35/745 Bifidobacteria
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor

Abstract

The invention relates to one or more systems and methods for creating a mixture of cultures used to replace intestinal flora. Specifically, the invention relates to methods and systems of treating diseases including Clostridium difficile and Crohn'"'"'s disease by introducing a mixture of pure cultures of viable bacteria into the gastrointestinal tract.

Claims

1. A system of replacing intestinal flora comprising growing a mixture of cultures which includes one or more species of Bacteroides and administering said mixture into a gastrointestinal system.

2. The system of claim 1 including Bacteroides thetaiotaomicron.

3. The system of claim 1 including Bacteroides fragilis.

4. The system of claim 1 including Bacteroides vulgatis.

5. The system of claim 1 including Bacteroides distatonis.

6. The system of claim 1 wherein the mixture of cultures further comprises Eubacterium cylindroides.

7. The system of claim 1 wherein the mixture of cultures further comprises Bifidobacterium bifidum.

8. The system of claim 1 wherein the mixture of cultures comprises a 300 ml infusion containing 1×

10̂

10 Bacteroides thetaiotaomicron, 1×

10̂

9 Eubacterium cylindroides, and 1×

10̂

9 Bifidobacterium bifidum.

9. The system of claim 1 wherein the mixture of cultures is administered via a nasoduodenal tube into a gastrointestinal system.

10. The system of claim 1 wherein the mixture of cultures is administered via a nasogastric tube into a gastrointestinal system.

11. The system of claim 1 wherein the mixture of cultures is administered via an enema into a gastrointestinal system.

12. A method of producing cultures used to restore intestinal flora comprising:

a. creating a mixture of cultures including Bacteroides thetaiotaomicron, Eubacterium cylindroides, and Bifidobacterium bifidum wherein each organism is cultured separately;

b. growing the cultures under anaerobic conditions;

c. After incubating the cultures, removing the liquid portion of the medium and diluting the culture with a medium suitable for growing cultures to create a final preparation.

13. The method of claim 12 wherein the mixture of cultures are grown for 48-72 hours.

14. The method of claim 12 wherein the final preparation is freeze-dried.

15. The method of claim 14 wherein the final preparation is rehydrated.

16. The method of claim 15 wherein the final preparation is rehydrated using a sodium chloride solution.