METHOD FOR DEPOLYMERISING POLYSACCHARIDES BY MEANS OF MECHANICAL MILLING

• US 20130032650A1
• Filed: 11/29/2010
• Published: 02/07/2013
• Est. Priority Date: 11/27/2009
• Status: Abandoned Application

• Current Assignee: Centre National De La Recherche Scientifique, Institut francais de recherche pour l'exploitation de la mer, University Pierre et Marie Curie
• Original Assignee: Centre National De La Recherche Scientifique, Institut Francais De Recherche Pour L'Exploitation De La Mer-Ifremer, University Pierre et Marie Curie
• Inventors: Boisset, Claire, Cozien, Joelle, Sinquin, Corinne, Ratiskol, Jacqueline, Helbert, William, Le Costaouec, Tinaig, Correc, Gaelle, Larreta Garde, Veronique
• Application Number:
  US13/509,029
• Publication Number:
  US 20130032650A1
• Time in Patent Office: Days
• US Class Current: 241/25
• CPC Class Codes: C08B 15/00 Preparation of other cellulose derivatives or modified cellulose , e.g. complexes
• CPC Class Codes: C08B 30/12 Degraded, destructured or non-chemically modified starch , e.g. mechanically, enzymatically or by irradiation; Bleaching of starch preparation of chemical derivatives of starch C08B31/00
• CPC Class Codes: C08B 35/00 Preparation of derivatives of amylopectin
• CPC Class Codes: C08B 37/0021 Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran
• CPC Class Codes: C08B 37/0024 beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
• CPC Class Codes: C08B 37/003 Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
• CPC Class Codes: C08B 37/0039 Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
• CPC Class Codes: C08B 37/0042 Carragenan or carragen, i.e. D-galactose and 3,6-anhydro-D-galactose, both partially sulfated, e.g. from red algae Chondrus crispus or Gigantia stellata; kappa-Carragenan; iota-Carragenan; lambda-Carragenan; Derivatives thereof
• CPC Class Codes: C08B 37/0045 alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
• CPC Class Codes: C08B 37/0054 Inulin, i.e. beta-2,1-D-fructofuranan; Derivatives thereof
• CPC Class Codes: C08B 37/006 Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
• CPC Class Codes: C08B 37/0069 Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof
• CPC Class Codes: C08B 37/0084 Guluromannuronans, e.g. alginic acid, i.e. D-mannuronic acid and D-guluronic acid units linked with alternating alpha- and beta-1,4-glycosidic bonds; Derivatives thereof, e.g. alginates
• CPC Class Codes: C08B 37/0096 Guar, guar gum, guar flour, guaran, i.e. (beta-1,4) linked D-mannose units in the main chain branched with D-galactose units in (alpha-1,6), e.g. from Cyamopsis Tetragonolobus; Derivatives thereof
• CPC Class Codes: C08H 8/00 Macromolecular compounds derived from lignocellulosic materials pretreatment thereof B27N

Abstract

A method for controlled reduction of the weight average molar mass of a polysaccharide down to a lower and determined weight average molar mass, which method includes a step for mechanical milling of the polysaccharide by a ball mill, until the polysaccharide having the desired weight average molar mass is obtained.

Claims

1. A method for controlled reduction in the weight average molar mass of a polysaccharide down to a lower and determined weight average molar mass, comprising a step for mechanical milling of said polysaccharide by means of a ball mill, and characterized in that the weight average molar mass of the polysaccharide before depolymerization (A₀), the weight average molar mass of the polysaccharide after depolymerization (A_t) and the milling time (t) are selected so that by solving the following equation:

A_t=A₀e<sup>−

kt</sup>−

200 t+X wherein

k is a constant comprised between 0.1 and 5, preferably 0.5 and 3, and

X=A₀/k′

with k′

comprised between 1 and 200, preferably between 10 and 100,

it is possible to obtain after depolymerization a polysaccharide having the desired weight average molar mass.

2. The method according to claim 1, characterized in that the polysaccharide is a polymer consisting of oses selected from the group comprising starch, polydextrose, lignocellulose, mannan, chitin, chitosan, cellulose, xylanes, amylopectin, pectins, galactomannans like guar gum, inulin, glucomannans, xanthans, gellans, alginates, chondroitins, hyaluronans, curdlans, pullulans, succinoglycans, laminarans, alternans, scleroglucans, dextrans, levans, fucans, carrageenans, agars and ulvans and derivatives thereof.

3. The method according to claim 1, characterized in that said method is applied to a polysaccharide in the solid phase.

4. The method according to claim 1, characterized in that the weight average molar mass of the polysaccharide before depolymerization is typically comprised between 3,000,000 and 30,000 g·

mol<sup>−

1</sup>.

5. The method according to claim 1, characterized in that the weight average molar mass of the polysaccharide after depolymerization is comprised between 500,000 g·

mol<sup>−

1</sup> and 8,000 g·

mol<sup>−

1</sup>, preferably between 1,000,000 g·

mol<sup>−

1</sup> and 1,000 g·

mol<sup>−

1</sup>.

6. The method according to claim 1, characterized in that said method does not comprise any additional purification step.

7. The method according to claim 1, characterized in that said method does not comprise any additional freeze-drying step.

8. The method according to claim 1, characterized in that said method does not comprise any additional step for stabilization of the obtained polysaccharide.

9. The method according to claim 1, characterized in that said polysaccharide has a hydration degree comprised between 0% and 100%, preferably between 10% and 50% and more preferably between 2% and 15%.

10. The method according to claim 1, characterized in that the obtained polysaccharide after depolymerization has a polydispersity index comprised between 1.1 and 5.