POPULATIONS OF PANCREATIC PROGENITOR CELLS AND METHODS OF ISOLATING AND USING SAME
First Claim
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1. A method of generating pancreatic cells the method comprising:
- (a) differentiating stem cells under conditions such that at least a portion of said cells express glucose transporter 2 (GLUT2) so as to generate GLUT2-expressing cells; and
(b) enriching for said GLUT2-expressing cells so as to generate a population of GLUT2 enriched cells, wherein at least 80% of said population of GLUT2 enriched cells express GLUT2, thereby generating pancreatic cells.
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Abstract
A method of generating pancreatic progenitor cells is disclosed. The method comprises:
- (a) differentiating stem cells under conditions such that at least a portion of the cells express glucose transporter 2 (GLUT2) so as to generate GLUT2-expressing cells; and
- (b) enriching for the GLUT2-expressing cells so as to generate a population of GLUT2 enriched cells, wherein at least 80% of the population of GLUT2 enriched cells express GLUT2, thereby generating pancreatic progenitor cells.
Isolated populations of cells generated according to the method, pharmaceutical compositions comprising same and uses thereof are also disclosed.
18 Citations
21 Claims
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1. A method of generating pancreatic cells the method comprising:
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(a) differentiating stem cells under conditions such that at least a portion of said cells express glucose transporter 2 (GLUT2) so as to generate GLUT2-expressing cells; and (b) enriching for said GLUT2-expressing cells so as to generate a population of GLUT2 enriched cells, wherein at least 80% of said population of GLUT2 enriched cells express GLUT2, thereby generating pancreatic cells. - View Dependent Claims (2, 3, 4, 5, 6, 10, 21)
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7. An isolated population of pancreatic cells generated by:
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(a) differentiating stem cells under conditions such that at least a portion of said cells express glucose transporter 2 (GLUT2) so as to generate GLUT2-expressing cells; and (b) enriching for said GLUT2-expressing cells so as to generate a population of GLUT2 enriched cells, wherein at least 80% of said population of GLUT2 enriched cells express GLUT2. - View Dependent Claims (8, 16)
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9. A pharmaceutical composition comprising an isolated population of pancreatic cells generated by:
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(a) differentiating stem cells under conditions such that at least a portion of said cells express glucose transporter 2 (GLUT2) so as to generate GLUT2-expressing cells; and (b) enriching for said GLUT2-expressing cells so as to generate a population of GLUT2 enriched cells, wherein at least 80% of said population of GLUT2 enriched cells express GLUT2. - View Dependent Claims (18)
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11-15. -15. (canceled)
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17. (canceled)
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19. A method of treating Diabetes in a subject in need thereof, the method comprising transplanting a therapeutically effective amount of an isolated population of pancreatic cells into the subject, wherein said cells are generated by:
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(a) differentiating stem cells under conditions such that at least a portion of said cells express glucose transporter 2 (GLUT2) so as to generate GLUT2-expressing cells; and (b) enriching for said GLUT2-expressing cells so as to generate a population of GLUT2 enriched cells, wherein at least 80% of said population of GLUT2 enriched cells express GLUT2, thereby treating the Diabetes. - View Dependent Claims (20)
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Specification