METHODS FOR ENGINEERING T-CELL RECEPTORS
First Claim
1. A method for engineering a T-cell receptor domain polypeptide comprising at least one modification in a structural loop region of said T-cell receptor domain polypeptide and determining the binding of said T-cell receptor domain polypeptide to an epitope of an antigen, wherein the unmodified T-cell receptor domain polypeptide does not significantly bind to said epitope, comprising the steps of:
- (a) providing a nucleic acid encoding a T-cell receptor domain polypeptide comprising at least one structural loop region,(b) modifying at least one nucleotide residue of at least one of said structural loop regions,(c) transferring said modified nucleic acid in an expression system,(d) expressing said modified T-cell receptor domain polypeptide,(e) contacting the expressed modified T-cell receptor domain polypeptide with said epitope and(f) determining whether said modified T-cell receptor domain polypeptide binds to said epitope.
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Abstract
The present invention provides a method for engineering a T-cell receptor domain polypeptide comprising at least one modification in a structural loop region of the T-cell receptor domain polypeptide and determining the binding of the T-cell receptor domain polypeptide to an epitope of an antigen, wherein the unmodified T-cell receptor domain polypeptide does not significantly bind to said epitope. The present invention also covers modified T cell receptor domain polypeptides, their use and libraries containing the modified T cell receptor domain polypeptides.
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Citations
27 Claims
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1. A method for engineering a T-cell receptor domain polypeptide comprising at least one modification in a structural loop region of said T-cell receptor domain polypeptide and determining the binding of said T-cell receptor domain polypeptide to an epitope of an antigen, wherein the unmodified T-cell receptor domain polypeptide does not significantly bind to said epitope, comprising the steps of:
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(a) providing a nucleic acid encoding a T-cell receptor domain polypeptide comprising at least one structural loop region, (b) modifying at least one nucleotide residue of at least one of said structural loop regions, (c) transferring said modified nucleic acid in an expression system, (d) expressing said modified T-cell receptor domain polypeptide, (e) contacting the expressed modified T-cell receptor domain polypeptide with said epitope and (f) determining whether said modified T-cell receptor domain polypeptide binds to said epitope. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 27)
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- 19. A T-cell receptor domain polypeptide comprising at least one structural loop region, said at least one loop region comprising at least one modification enabling binding of said at least one modified loop region to an epitope of an antigen wherein the unmodified T-cell receptor domain polypeptide does not bind to said epitope.
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26. A nucleic acid encoding a T-cell receptor according to claim 26, or part thereof.
Specification