SACCHARIFIED-SOLUTION MANUFACTURING METHOD AND SACCHARIFIED-SOLUTION MANUFACTURING DEVICE USED IN SAID METHOD

• US 20130059347A1
• Filed: 05/12/2011
• Published: 03/07/2013
• Est. Priority Date: 05/12/2010
• Status: Abandoned Application

• Current Assignee: Honda Motor Co., Ltd. (Honda Motor Company)
• Original Assignee: Honda Motor Co., Ltd. (Honda Motor Company)
• Inventors: Baba, Tsuyoshi, Uda, Makoto, Isobe, Shoji, Imai, Tomohiro
• Application Number:
  US13/697,334
• Publication Number:
  US 20130059347A1
• Time in Patent Office: Days
• US Class Current: 435/99
• CPC Class Codes: C12M 21/18 Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
• CPC Class Codes: C12M 23/02 Form or structure of the vessel large containers B65D88/00
• CPC Class Codes: C12M 45/06 by chemical means or hydrolysis
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C13K 1/02 obtained by saccharification of cellulosic materials manufacture of fodder A23K10/32

Abstract

[Problems to be Solved]

Provide is a saccharified-solution manufacturing method and a saccharified-solution manufacturing device, which increases the amount of sugar yielded as a saccharified-solution, when lignocellulose-based biomass is subjected to saccharifying enzyme treatment.

[Solution]

A substrate and ammonia water are mixed at a predetermined mass ratio to yield a substrate mixture, the mixture is retained at a predetermined temperature for a predetermined time period for dissociating lignin from the substrate or swelling the substrate to yield a pretreated ammonia-containing product for saccharification. To a pretreated product for saccharification, which is yielded by separating ammonia from the pretreated ammonia-containing product for saccharification, an acid is added for pH adjustment, and also a saccharifying enzyme is added to yield a substrate/saccharifying enzyme mixture liquid containing a substrate of 15 to 30% by mass. The substrate/saccharifying enzyme mixture liquid is saccharified enzymatically to yield a saccharified-solution.

Claims

1. A saccharified-solution manufacturing method, in which lignocellulose-based biomass as a substrate is pretreated prior to saccharification to yield a pretreated product for saccharification in which lignin is dissociated from the substrate or the substrate is swollen, and thereafter the pretreated product for saccharification is saccharified enzymatically to yield a saccharified-solution, wherein the saccharified-solution manufacturing method comprises:

a step of mixing the substrate and ammonia water having a concentration in a range of 20 to 30% by mass at a mass ratio in a range of substrate;

ammonia water=1;

0.7 to 1;

1.3 to yield a substrate mixture;

a step of heating and retaining the substrate mixture at a temperature in a range of 25 to 100°

C. for a time period in a range of 1 to 100 hours to dissociate lignin from the substrate or to swell the substrate to yield a pretreated ammonia-containing product for saccharification;

a step of separating ammonia from the pretreated ammonia-containing product for saccharification through natural emission of ammonia gas to yield a substantially non-flowable pretreated product for saccharification;

a step of adding at least one acid of phosphoric acid, nitric acid and sulfuric acid to the pretreated product for saccharification after the ammonia separation to adjust a pH of the pretreated product for saccharification in a range of 3 to 7 and adding a saccharifying enzyme to yield a substrate/saccharifying enzyme mixture liquid containing the substrate in a range of 15 to 30% by mass with respect to the total mass;

a step of saccharifying enzymatically the substrate/saccharifying enzyme mixture liquid into a flowable state to yield a first saccharification treatment product;

a step of transferring the first saccharification treatment product to a next step under non-air-contact condition; and

a step of saccharifying enzymatically the first saccharification treatment product to yield a saccharified-solution as a second saccharification treatment product.

3. The saccharified-solution manufacturing method according to claim 1, wherein the substrate/saccharifying enzyme mixture liquid is saccharified enzymatically into a flowable state using an enzyme for degrading cellulose and hemicellulose.

4. The saccharified-solution manufacturing method according to claim 1, wherein the first saccharification treatment product saccharified into a flowable state is saccharified enzymatically using an enzyme for degrading cellulose and hemicellulose.

5. The saccharified-solution manufacturing method according to claim 1, wherein the substrate/saccharifying enzyme mixture liquid is saccharified into a flowable state, which is transferred, when a viscosity of a first saccharification treatment product reaches a range of 30 to 1000 Pa·

s, to the step of yielding a saccharified-solution.

2. (canceled)

6. A saccharified-solution manufacturing device, in which lignocellulose-based biomass as a substrate is pretreated prior to saccharification to yield to yield a pretreated product for saccharification in which lignin is dissociated from the substrate or the substrate is swollen, and thereafter the pretreated product for saccharification is saccharified enzymatically to yield a saccharified-solution, wherein the saccharified-solution manufacturing device comprises:

a first saccharification treatment unit configured;

to mix the substrate and ammonia water having a concentration in a range of 20 to 30% by mass at a mass ratio in a range of substrate;

ammonia water=1;

0.7 to 1;

1.3 to yield a substrate mixture;

to heat and retain the yielded substrate mixture at a temperature in a range of 25 to 100°

C. for a time period in a range of 1 to 100 hours to dissociate lignin from the substrate or to swell the substrate to yield a pretreated ammonia-containing product for saccharification;

to separate ammonia from the yielded pretreated ammonia-containing product for saccharification through natural emission of ammonia gas to yield a substantially non-flowable pretreated product for saccharification;

to adjust a pH of the pretreated product for saccharification after the ammonia separation in a range of 3 to 7 and add a saccharifying enzyme to yield a substrate/saccharifying enzyme mixture liquid containing the substrate in a range of 15 to 30% by mass with respect to the total mass; and

to saccharify enzymatically the substrate/saccharifying enzyme mixture liquid into a flowable state to yield a first saccharification treatment product;

an ammonia water supply unit configured to supply the ammonia water to the first saccharification treatment unit;

a pH adjustment unit configured to adjust the pH of the pretreated product for saccharification in the above range by adding at least one acid of phosphoric acid, nitric acid and sulfuric acid to the first saccharification treatment unit;

a saccharifying enzyme addition unit configured to add a saccharifying enzyme to the first saccharification treatment unit;

a transfer unit configured to transfer the first saccharification treatment product without contacting the outside air; and

a second saccharification treatment unit configured to perform an enzymatic saccharification treatment of the first saccharification treatment product transferred by the transfer unit to yield a saccharified-solution as a second saccharification treatment product.