METHOD AND APPARATUS FOR PREPARING NOVEL LIPOSOME
First Claim
1. A method for preparing liposome formulation, the method comprising the steps of:
- (a) dissolving a lipid fraction comprising at least one kind selected from phospholipid and sterol, and a bioactive substance in an organic solvent;
(b) putting an organic solvent solution of the step (a) together with a saccharide carrier into a reaction vessel, and introducing a supercritical fluid thereto so as to prepare proliposome particles coated with a lipid-bioactive substance mixture;
(c) discharging the supercritical fluid in step (b) by compression so as to obtain the proliposome particles; and
(d) hydrating the proliposome particles in step (c) by an aqueous solution comprising water so as to form a liposome solution.
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Abstract
Disclosed is a method for preparing a liposome formulation. In the disclosed method, a lipid fraction is dissolved in an organic solvent. The solution including a bioactive component and the lipid fraction, together with a carrier, is put in a reaction vessel, and a supercritical fluid is introduced thereto, so as to prepare particles coated with the bioactive component-lipid. The supercritical fluid is discharged by compression to obtain proliposome particles, and then the proliposome particles are hydrated by an aqueous solution including water so as to form a liposome solution. Preferably, the formulation may include one or more bioactive components. As required, the liposome formulation may be further processed by methods such as particle size reduction, removal of organic solvent, and freeze-drying. The preparation method can be easily carried out at a laboratory scale. Furthermore, the same method can be employed in liposome formulation preparation in mass production, or at a commercial scale.
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Citations
13 Claims
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1. A method for preparing liposome formulation, the method comprising the steps of:
- (a) dissolving a lipid fraction comprising at least one kind selected from phospholipid and sterol, and a bioactive substance in an organic solvent;
(b) putting an organic solvent solution of the step (a) together with a saccharide carrier into a reaction vessel, and introducing a supercritical fluid thereto so as to prepare proliposome particles coated with a lipid-bioactive substance mixture;
(c) discharging the supercritical fluid in step (b) by compression so as to obtain the proliposome particles; and
(d) hydrating the proliposome particles in step (c) by an aqueous solution comprising water so as to form a liposome solution. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
- (a) dissolving a lipid fraction comprising at least one kind selected from phospholipid and sterol, and a bioactive substance in an organic solvent;
Specification